Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions

Zhuoheng Zhong, Takashi Furuya, Kimitaka Ueno, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida, Masahiko Tani, Jingkui Tian, Setsuko Komatsu

Research output: Contribution to journalArticle

Abstract

Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1-10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways.

Original languageEnglish
JournalInternational journal of molecular sciences
Volume21
Issue number2
DOIs
Publication statusPublished - 12-01-2020

Fingerprint

soybeans
Soybeans
Millimeter waves
Proteomics
millimeter waves
Irradiation
irradiation
glycolysis
Growth
Seedlings
Oxidative stress
Glycolysis
Metabolism
metabolism
Trehalose
Oxidation-Reduction
Oxidative Stress
Sugars
Frequency bands
Glutathione

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Zhong, Zhuoheng ; Furuya, Takashi ; Ueno, Kimitaka ; Yamaguchi, Hisateru ; Hitachi, Keisuke ; Tsuchida, Kunihiro ; Tani, Masahiko ; Tian, Jingkui ; Komatsu, Setsuko. / Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions. In: International journal of molecular sciences. 2020 ; Vol. 21, No. 2.
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Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions. / Zhong, Zhuoheng; Furuya, Takashi; Ueno, Kimitaka; Yamaguchi, Hisateru; Hitachi, Keisuke; Tsuchida, Kunihiro; Tani, Masahiko; Tian, Jingkui; Komatsu, Setsuko.

In: International journal of molecular sciences, Vol. 21, No. 2, 12.01.2020.

Research output: Contribution to journalArticle

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