Proteomic analysis of the effect of inorganic and organic chemicals on silver nanoparticles in wheat

Hafiz Muhammad Jhanzab, Abdul Razzaq, Yamin Bibi, Farhat Yasmeen, Hisateru Yamaguchi, Keisuke Hitachi, Kunihiro Tsuchida, Setsuko Komatsu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Production and utilization of nanoparticles (NPs) are increasing due to their positive and stimulating effects on biological systems. Silver (Ag) NPs improve seed germination, photosynthetic efficiency, plant growth, and antimicrobial activities. In this study, the effects of chemo-blended Ag NPs on wheat were investigated using the gel-free/label-free proteomic technique. Morphological analysis revealed that chemo-blended Ag NPs resulted in the increase of shoot length, shoot fresh weight, root length, and root fresh weight. Proteomic analysis indicated that proteins related to photosynthesis and protein synthesis were increased, while glycolysis, signaling, and cell wall related proteins were decreased. Proteins related to redox and mitochondrial electron transport chain were also decreased. Glycolysis associated proteins such as glyceraldehyde-3-phosphate dehydrogenase increased as well as decreased, while phosphoenol pyruvate carboxylase was decreased. Antioxidant enzyme activities such as superoxide dismutase, catalase, and peroxidase were promoted in response to the chemo-blended Ag NPs. These results suggested that chemo-blended Ag NPs promoted plant growth and development through regulation of energy metabolism by suppression of glycolysis. Number of grains/spike, 100-grains weight, and yield of wheat were stimulated with chemo-blended Ag NPs. Morphological study of next generational wheat plants depicted normal growth, and no toxic effects were observed. Therefore, morphological, proteomic, yield, and next generation results revealed that chemo-blended Ag NPs may promote plant growth and development through alteration in plant metabolism.

Original languageEnglish
Article number825
JournalInternational journal of molecular sciences
Volume20
Issue number4
DOIs
Publication statusPublished - 02-02-2019

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Inorganic Chemicals
Inorganic chemicals
Organic Chemicals
wheat
Organic chemicals
Silver
Proteomics
Nanoparticles
Triticum
silver
nanoparticles
glycolysis
Proteins
Glycolysis
proteins
Plant Development
metabolism
Growth and Development
Weights and Measures
Pyruvate Carboxylase

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

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abstract = "Production and utilization of nanoparticles (NPs) are increasing due to their positive and stimulating effects on biological systems. Silver (Ag) NPs improve seed germination, photosynthetic efficiency, plant growth, and antimicrobial activities. In this study, the effects of chemo-blended Ag NPs on wheat were investigated using the gel-free/label-free proteomic technique. Morphological analysis revealed that chemo-blended Ag NPs resulted in the increase of shoot length, shoot fresh weight, root length, and root fresh weight. Proteomic analysis indicated that proteins related to photosynthesis and protein synthesis were increased, while glycolysis, signaling, and cell wall related proteins were decreased. Proteins related to redox and mitochondrial electron transport chain were also decreased. Glycolysis associated proteins such as glyceraldehyde-3-phosphate dehydrogenase increased as well as decreased, while phosphoenol pyruvate carboxylase was decreased. Antioxidant enzyme activities such as superoxide dismutase, catalase, and peroxidase were promoted in response to the chemo-blended Ag NPs. These results suggested that chemo-blended Ag NPs promoted plant growth and development through regulation of energy metabolism by suppression of glycolysis. Number of grains/spike, 100-grains weight, and yield of wheat were stimulated with chemo-blended Ag NPs. Morphological study of next generational wheat plants depicted normal growth, and no toxic effects were observed. Therefore, morphological, proteomic, yield, and next generation results revealed that chemo-blended Ag NPs may promote plant growth and development through alteration in plant metabolism.",
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Proteomic analysis of the effect of inorganic and organic chemicals on silver nanoparticles in wheat. / Jhanzab, Hafiz Muhammad; Razzaq, Abdul; Bibi, Yamin; Yasmeen, Farhat; Yamaguchi, Hisateru; Hitachi, Keisuke; Tsuchida, Kunihiro; Komatsu, Setsuko.

In: International journal of molecular sciences, Vol. 20, No. 4, 825, 02.02.2019.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Jhanzab, Hafiz Muhammad

AU - Razzaq, Abdul

AU - Bibi, Yamin

AU - Yasmeen, Farhat

AU - Yamaguchi, Hisateru

AU - Hitachi, Keisuke

AU - Tsuchida, Kunihiro

AU - Komatsu, Setsuko

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