Abstract
We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to Someya et al. (2021).
Original language | English |
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Article number | 101341 |
Journal | STAR Protocols |
Volume | 3 |
Issue number | 2 |
DOIs | |
Publication status | Published - 17-06-2022 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- General Neuroscience
- General Biochemistry,Genetics and Molecular Biology
- General Immunology and Microbiology