Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy

Hiromi Nakai-Shimoda; Tatsuhito Himeno; Mikio Motegi; Norio Ozeki; Rieko Inoue; Tomohide Hayami; Emiri Miura-Yura; Yuichiro Yamada; Yoshiaki Morishita; Shin Tsunekawa; Yoshiro Kato; Yusuke Seino; Masaki Kondo; Keiko Naruse; Koichi Kato; Hiroki Mizukami; Jiro Nakamura; Hideki Kamiya

doi:10.1016/j.xpro.2022.101591

Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy

Hiromi Nakai-Shimoda
, Tatsuhito Himeno
, Mikio Motegi
, Norio Ozeki
, Rieko Inoue
, Tomohide Hayami
, Emiri Miura-Yura
, Yuichiro Yamada
, Yoshiaki Morishita
, Shin Tsunekawa
, Yoshiro Kato
, Yusuke Seino
, Masaki Kondo
, Keiko Naruse
, Koichi Kato
, Hiroki Mizukami
, Jiro Nakamura
, Hideki Kamiya

Research output: Contribution to journal › Article › peer-review

Abstract

Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detail the procedures of sampling, fixation, and embedding of peripheral nerves. We then outline the steps for ultrathin sectioning and transmission electron microscopy imaging. Finally, we describe morphological evaluation of nerve fibers in these images using ImageJ and AxonSeg. For complete details on the use and execution of this protocol, please refer to Nakai-Shimoda et al. (2021).

Original language	English
Article number	101591
Journal	STAR Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2022.101591
Publication status	Published - 16-09-2022
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2022.101591

Cite this

Nakai-Shimoda, H., Himeno, T., Motegi, M., Ozeki, N., Inoue, R., Hayami, T., Miura-Yura, E., Yamada, Y., Morishita, Y., Tsunekawa, S., Kato, Y., Seino, Y., Kondo, M., Naruse, K., Kato, K., Mizukami, H., Nakamura, J., & Kamiya, H. (2022). Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy. STAR Protocols, 3(3), Article 101591. https://doi.org/10.1016/j.xpro.2022.101591

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title = "Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy",

abstract = "Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detail the procedures of sampling, fixation, and embedding of peripheral nerves. We then outline the steps for ultrathin sectioning and transmission electron microscopy imaging. Finally, we describe morphological evaluation of nerve fibers in these images using ImageJ and AxonSeg. For complete details on the use and execution of this protocol, please refer to Nakai-Shimoda et al. (2021).",

keywords = "Metabolism, Microscopy, Neuroscience",

author = "Hiromi Nakai-Shimoda and Tatsuhito Himeno and Mikio Motegi and Norio Ozeki and Rieko Inoue and Tomohide Hayami and Emiri Miura-Yura and Yuichiro Yamada and Yoshiaki Morishita and Shin Tsunekawa and Yoshiro Kato and Yusuke Seino and Masaki Kondo and Keiko Naruse and Koichi Kato and Hiroki Mizukami and Jiro Nakamura and Hideki Kamiya",

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year = "2022",

month = sep,

day = "16",

doi = "10.1016/j.xpro.2022.101591",

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Nakai-Shimoda, H, Himeno, T, Motegi, M, Ozeki, N, Inoue, R, Hayami, T, Miura-Yura, E, Yamada, Y, Morishita, Y, Tsunekawa, S, Kato, Y, Seino, Y, Kondo, M, Naruse, K, Kato, K, Mizukami, H, Nakamura, J & Kamiya, H 2022, 'Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy', STAR Protocols, vol. 3, no. 3, 101591. https://doi.org/10.1016/j.xpro.2022.101591

TY - JOUR

T1 - Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy

AU - Nakai-Shimoda, Hiromi

AU - Himeno, Tatsuhito

AU - Motegi, Mikio

AU - Ozeki, Norio

AU - Inoue, Rieko

AU - Hayami, Tomohide

AU - Miura-Yura, Emiri

AU - Yamada, Yuichiro

AU - Morishita, Yoshiaki

AU - Tsunekawa, Shin

AU - Kato, Yoshiro

AU - Seino, Yusuke

AU - Kondo, Masaki

AU - Naruse, Keiko

AU - Kato, Koichi

AU - Mizukami, Hiroki

AU - Nakamura, Jiro

AU - Kamiya, Hideki

PY - 2022/9/16

Y1 - 2022/9/16

N2 - Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detail the procedures of sampling, fixation, and embedding of peripheral nerves. We then outline the steps for ultrathin sectioning and transmission electron microscopy imaging. Finally, we describe morphological evaluation of nerve fibers in these images using ImageJ and AxonSeg. For complete details on the use and execution of this protocol, please refer to Nakai-Shimoda et al. (2021).

AB - Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detail the procedures of sampling, fixation, and embedding of peripheral nerves. We then outline the steps for ultrathin sectioning and transmission electron microscopy imaging. Finally, we describe morphological evaluation of nerve fibers in these images using ImageJ and AxonSeg. For complete details on the use and execution of this protocol, please refer to Nakai-Shimoda et al. (2021).

KW - Metabolism

KW - Microscopy

KW - Neuroscience

UR - https://www.scopus.com/pages/publications/85135548625

UR - https://www.scopus.com/pages/publications/85135548625#tab=citedBy

U2 - 10.1016/j.xpro.2022.101591

DO - 10.1016/j.xpro.2022.101591

M3 - Article

C2 - 35942346

AN - SCOPUS:85135548625

SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 3

M1 - 101591

ER -

Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy

Abstract

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