Purification and characterization of human RNPS1: A general activator of pre-mRNA splicing

Akila Mayeda, Joseph Badolato, Ryuji Kobayashi, Michael Q. Zhang, Edith M. Gardiner, Adrian R. Krainer

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116 Citations (Scopus)

Abstract

Biochemical purification of a pre-mRNA splicing activity from HeLa cells that stimulates distal alternative 3' splice sites in a concentration-dependent manner resulted in the identification of RNPS1, a novel general activator of pre-mRNA splicing. RNPS1 cDNAs, encoding a putative nucleic-acid-binding protein of unknown function, were previously identified in mouse and human. RNPS1 is conserved in metazoans and has an RNA-recognition motif preceded by an extensive serine-rich domain. Recombinant human RNPS1 expressed in baculovirus functionally synergizes with SR proteins and strongly activates splicing of both constitutively and alternatively spliced pre-mRNAs. We conclude that RNPS1 is not only a potential regulator of alternative splicing but may also play a more fundamental role as a general activator of pre-mRNA splicing.

Original languageEnglish
Pages (from-to)4560-4570
Number of pages11
JournalEMBO Journal
Volume18
Issue number16
DOIs
Publication statusPublished - 16-08-1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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    Mayeda, A., Badolato, J., Kobayashi, R., Zhang, M. Q., Gardiner, E. M., & Krainer, A. R. (1999). Purification and characterization of human RNPS1: A general activator of pre-mRNA splicing. EMBO Journal, 18(16), 4560-4570. https://doi.org/10.1093/emboj/18.16.4560