Purification and characterization of human RNPS1: A general activator of pre-mRNA splicing

Akira Maeda, Joseph Badolato, Ryuji Kobayashi, Michael Q. Zhang, Edith M. Gardiner, Adrian R. Krainer

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Biochemical purification of a pre-mRNA splicing activity from HeLa cells that stimulates distal alternative 3' splice sites in a concentration-dependent manner resulted in the identification of RNPS1, a novel general activator of pre-mRNA splicing. RNPS1 cDNAs, encoding a putative nucleic-acid-binding protein of unknown function, were previously identified in mouse and human. RNPS1 is conserved in metazoans and has an RNA-recognition motif preceded by an extensive serine-rich domain. Recombinant human RNPS1 expressed in baculovirus functionally synergizes with SR proteins and strongly activates splicing of both constitutively and alternatively spliced pre-mRNAs. We conclude that RNPS1 is not only a potential regulator of alternative splicing but may also play a more fundamental role as a general activator of pre-mRNA splicing.

Original languageEnglish
Pages (from-to)4560-4570
Number of pages11
JournalEMBO Journal
Volume18
Issue number16
DOIs
Publication statusPublished - 16-08-1999

Fingerprint

RNA Precursors
Purification
RNA Splice Sites
Baculoviridae
Alternative Splicing
HeLa Cells
Serine
Nucleic Acids
Carrier Proteins
Complementary DNA
RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Maeda, A., Badolato, J., Kobayashi, R., Zhang, M. Q., Gardiner, E. M., & Krainer, A. R. (1999). Purification and characterization of human RNPS1: A general activator of pre-mRNA splicing. EMBO Journal, 18(16), 4560-4570. https://doi.org/10.1093/emboj/18.16.4560
Maeda, Akira ; Badolato, Joseph ; Kobayashi, Ryuji ; Zhang, Michael Q. ; Gardiner, Edith M. ; Krainer, Adrian R. / Purification and characterization of human RNPS1 : A general activator of pre-mRNA splicing. In: EMBO Journal. 1999 ; Vol. 18, No. 16. pp. 4560-4570.
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Maeda, A, Badolato, J, Kobayashi, R, Zhang, MQ, Gardiner, EM & Krainer, AR 1999, 'Purification and characterization of human RNPS1: A general activator of pre-mRNA splicing', EMBO Journal, vol. 18, no. 16, pp. 4560-4570. https://doi.org/10.1093/emboj/18.16.4560

Purification and characterization of human RNPS1 : A general activator of pre-mRNA splicing. / Maeda, Akira; Badolato, Joseph; Kobayashi, Ryuji; Zhang, Michael Q.; Gardiner, Edith M.; Krainer, Adrian R.

In: EMBO Journal, Vol. 18, No. 16, 16.08.1999, p. 4560-4570.

Research output: Contribution to journalArticle

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