Purification, crystallization and preliminary X-ray analysis of the PCNA2-PCNA3 complex from Sulfolobus tokodaii strain 7

Akito Kawai; Shigesada Higuchi; Masaru Tsunoda; Kazuo T. Nakamura; Shuichi Miyamoto

doi:10.1107/S1744309109044479

Purification, crystallization and preliminary X-ray analysis of the PCNA2-PCNA3 complex from Sulfolobus tokodaii strain 7

Akito Kawai, Shigesada Higuchi, Masaru Tsunoda, Kazuo T. Nakamura, Shuichi Miyamoto

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Crenarchaeal PCNA is known to consist of three subunits (PCNA1, PCNA2 and PCNA3) that form a heterotrimer (PCNA123). Recently, another heterotrimeric PCNA composed of only PCNA2 and PCNA3 was identified in Sulfolobus tokodaii strain 7 (stoPCNAs). In this study, the purified stoPCNA2-stoPCNA3 complex was crystallized by hanging-drop vapour diffusion. The crystals obtained belonged to the orthorhombic space groups I222 and P2₁2₁2, with unit-cell parameters a = 91.1, b = 111.8, c = 170.9 Å and a = 91.1, b = 160.6, c = 116.6 Å, respectively. X-ray diffraction data sets were collected to 2.90 Å resolution for the I222 crystals and to 2.80 Å resolution for the P2₁2₁2 crystals.

Original language	English
Pages (from-to)	1282-1284
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	65
Issue number	12
DOIs	https://doi.org/10.1107/S1744309109044479
Publication status	Published - 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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title = "Purification, crystallization and preliminary X-ray analysis of the PCNA2-PCNA3 complex from Sulfolobus tokodaii strain 7",

abstract = "Crenarchaeal PCNA is known to consist of three subunits (PCNA1, PCNA2 and PCNA3) that form a heterotrimer (PCNA123). Recently, another heterotrimeric PCNA composed of only PCNA2 and PCNA3 was identified in Sulfolobus tokodaii strain 7 (stoPCNAs). In this study, the purified stoPCNA2-stoPCNA3 complex was crystallized by hanging-drop vapour diffusion. The crystals obtained belonged to the orthorhombic space groups I222 and P21212, with unit-cell parameters a = 91.1, b = 111.8, c = 170.9 {\AA} and a = 91.1, b = 160.6, c = 116.6 {\AA}, respectively. X-ray diffraction data sets were collected to 2.90 {\AA} resolution for the I222 crystals and to 2.80 {\AA} resolution for the P21212 crystals.",

author = "Akito Kawai and Shigesada Higuchi and Masaru Tsunoda and Nakamura, {Kazuo T.} and Shuichi Miyamoto",

year = "2009",

doi = "10.1107/S1744309109044479",

language = "English",

volume = "65",

pages = "1282--1284",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "John Wiley and Sons Ltd",

number = "12",

}

Purification, crystallization and preliminary X-ray analysis of the PCNA2-PCNA3 complex from Sulfolobus tokodaii strain 7. / Kawai, Akito; Higuchi, Shigesada; Tsunoda, Masaru et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 65, No. 12, 2009, p. 1282-1284.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Purification, crystallization and preliminary X-ray analysis of the PCNA2-PCNA3 complex from Sulfolobus tokodaii strain 7

AU - Kawai, Akito

AU - Higuchi, Shigesada

AU - Tsunoda, Masaru

AU - Nakamura, Kazuo T.

AU - Miyamoto, Shuichi

PY - 2009

Y1 - 2009

N2 - Crenarchaeal PCNA is known to consist of three subunits (PCNA1, PCNA2 and PCNA3) that form a heterotrimer (PCNA123). Recently, another heterotrimeric PCNA composed of only PCNA2 and PCNA3 was identified in Sulfolobus tokodaii strain 7 (stoPCNAs). In this study, the purified stoPCNA2-stoPCNA3 complex was crystallized by hanging-drop vapour diffusion. The crystals obtained belonged to the orthorhombic space groups I222 and P21212, with unit-cell parameters a = 91.1, b = 111.8, c = 170.9 Å and a = 91.1, b = 160.6, c = 116.6 Å, respectively. X-ray diffraction data sets were collected to 2.90 Å resolution for the I222 crystals and to 2.80 Å resolution for the P21212 crystals.

AB - Crenarchaeal PCNA is known to consist of three subunits (PCNA1, PCNA2 and PCNA3) that form a heterotrimer (PCNA123). Recently, another heterotrimeric PCNA composed of only PCNA2 and PCNA3 was identified in Sulfolobus tokodaii strain 7 (stoPCNAs). In this study, the purified stoPCNA2-stoPCNA3 complex was crystallized by hanging-drop vapour diffusion. The crystals obtained belonged to the orthorhombic space groups I222 and P21212, with unit-cell parameters a = 91.1, b = 111.8, c = 170.9 Å and a = 91.1, b = 160.6, c = 116.6 Å, respectively. X-ray diffraction data sets were collected to 2.90 Å resolution for the I222 crystals and to 2.80 Å resolution for the P21212 crystals.

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Purification, crystallization and preliminary X-ray analysis of the PCNA2-PCNA3 complex from Sulfolobus tokodaii strain 7

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