Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7

Akito Kawai; Shigesada Higuchi; Masaru Tsunoda; Kazuo T. Nakamura; Shuichi Miyamoto

doi:10.1107/S1744309112030278

Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7

Akito Kawai, Shigesada Higuchi, Masaru Tsunoda, Kazuo T. Nakamura, Shuichi Miyamoto

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDG) and stoUDG complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDG and stoUDG complexed with uracil, respectively.

Original language	English
Pages (from-to)	1102-1105
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	68
Issue number	9
DOIs	https://doi.org/10.1107/S1744309112030278
Publication status	Published - 09-2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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title = "Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7",

abstract = "Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDG) and stoUDG complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P212121, with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 {\AA} and a = 52.1, b = 52.2, c = 74.1 {\AA} for apo stoUDG and stoUDG complexed with uracil, respectively.",

keywords = "Sulfolobus tokodaii strain 7, family 4 uracil-DNA glycosylases",

author = "Akito Kawai and Shigesada Higuchi and Masaru Tsunoda and Nakamura, {Kazuo T.} and Shuichi Miyamoto",

year = "2012",

month = sep,

doi = "10.1107/S1744309112030278",

language = "English",

volume = "68",

pages = "1102--1105",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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}

Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7. / Kawai, Akito; Higuchi, Shigesada; Tsunoda, Masaru et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 68, No. 9, 09.2012, p. 1102-1105.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7

AU - Kawai, Akito

AU - Higuchi, Shigesada

AU - Tsunoda, Masaru

AU - Nakamura, Kazuo T.

AU - Miyamoto, Shuichi

PY - 2012/9

Y1 - 2012/9

N2 - Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDG) and stoUDG complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P212121, with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDG and stoUDG complexed with uracil, respectively.

AB - Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDG) and stoUDG complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P212121, with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDG and stoUDG complexed with uracil, respectively.

KW - Sulfolobus tokodaii strain 7

KW - family 4 uracil-DNA glycosylases

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Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7

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