TY - JOUR
T1 - Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7
AU - Kawai, Akito
AU - Higuchi, Shigesada
AU - Tsunoda, Masaru
AU - Nakamura, Kazuo T.
AU - Miyamoto, Shuichi
PY - 2012/9
Y1 - 2012/9
N2 - Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDG) and stoUDG complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P212121, with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDG and stoUDG complexed with uracil, respectively.
AB - Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDG) and stoUDG complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P212121, with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDG and stoUDG complexed with uracil, respectively.
KW - Sulfolobus tokodaii strain 7
KW - family 4 uracil-DNA glycosylases
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U2 - 10.1107/S1744309112030278
DO - 10.1107/S1744309112030278
M3 - Article
C2 - 22949205
AN - SCOPUS:84865772829
SN - 1744-3091
VL - 68
SP - 1102
EP - 1105
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 9
ER -