Quality assurance of quantitative PCR with real-time reverse transcription polymerase chain reaction method

Tadao Funato, Mayu Takeda

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The real-time reverse transcription polymerase chain reaction (RT-PCR) method is the most sensitive method for the detection of mRNA and DNA virus. The use of this real-time quantitative PCR(RQ-PCR) has been utilized increasingly to monitor gene expression of many types on clinical diagnosis, and has become standard for the detection and quantification of RNA targets. The employment of the techniques for RQ-PCR offers the advantages of high sensitivity and reproducibility. A number of RQ-PCR have been described that commercial PCR kits are available for quantitative analysis of a limited number of clinically important virus only. Quality assurance is necessary to be assessment the overall process and procedures of quantitative PCR as well as the other clinical testing. Therefore, it should be examined to be used with based on appropriately quality control.

Original languageEnglish
Pages (from-to)910-917
Number of pages8
JournalRinsho byori. The Japanese journal of clinical pathology
Volume54
Issue number9
Publication statusPublished - 09-2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Fingerprint Dive into the research topics of 'Quality assurance of quantitative PCR with real-time reverse transcription polymerase chain reaction method'. Together they form a unique fingerprint.

Cite this