Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay

Makito Tanaka; Yoshiyuki Takahashi; Yin Yan Xu; Nao Yoshida; Ayami Yoshimi; Itzel Bustos Villalobos; Asahito Hama; Nobuhiro Nishio; Hirokazu Hidaka; Yue Wang; Hiroshi Yagasaki; Seiji Kojima

doi:10.1016/j.leukres.2007.11.016

Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay

Makito Tanaka, Yoshiyuki Takahashi, Yin Yan Xu, Nao Yoshida, Ayami Yoshimi, Itzel Bustos Villalobos, Asahito Hama, Nobuhiro Nishio, Hirokazu Hidaka, Yue Wang, Hiroshi Yagasaki, Seiji Kojima

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a ³H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

Original language	English
Pages (from-to)	1036-1042
Number of pages	7
Journal	Leukemia Research
Volume	32
Issue number	7
DOIs	https://doi.org/10.1016/j.leukres.2007.11.016
Publication status	Published - 01-07-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Hematology
Oncology
Cancer Research

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10.1016/j.leukres.2007.11.016

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Tanaka, M., Takahashi, Y., Xu, Y. Y., Yoshida, N., Yoshimi, A., Villalobos, I. B., Hama, A., Nishio, N., Hidaka, H., Wang, Y., Yagasaki, H., & Kojima, S. (2008). Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay. Leukemia Research, 32(7), 1036-1042. https://doi.org/10.1016/j.leukres.2007.11.016

Tanaka, Makito ; Takahashi, Yoshiyuki ; Xu, Yin Yan ; Yoshida, Nao ; Yoshimi, Ayami ; Villalobos, Itzel Bustos ; Hama, Asahito ; Nishio, Nobuhiro ; Hidaka, Hirokazu ; Wang, Yue ; Yagasaki, Hiroshi ; Kojima, Seiji. / Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay. In: Leukemia Research. 2008 ; Vol. 32, No. 7. pp. 1036-1042.

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title = "Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay",

abstract = "Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.",

author = "Makito Tanaka and Yoshiyuki Takahashi and Xu, {Yin Yan} and Nao Yoshida and Ayami Yoshimi and Villalobos, {Itzel Bustos} and Asahito Hama and Nobuhiro Nishio and Hirokazu Hidaka and Yue Wang and Hiroshi Yagasaki and Seiji Kojima",

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doi = "10.1016/j.leukres.2007.11.016",

language = "English",

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Tanaka, M, Takahashi, Y, Xu, YY, Yoshida, N, Yoshimi, A, Villalobos, IB, Hama, A, Nishio, N, Hidaka, H, Wang, Y, Yagasaki, H & Kojima, S 2008, 'Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay', Leukemia Research, vol. 32, no. 7, pp. 1036-1042. https://doi.org/10.1016/j.leukres.2007.11.016

Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay. / Tanaka, Makito; Takahashi, Yoshiyuki; Xu, Yin Yan; Yoshida, Nao; Yoshimi, Ayami; Villalobos, Itzel Bustos; Hama, Asahito; Nishio, Nobuhiro; Hidaka, Hirokazu; Wang, Yue; Yagasaki, Hiroshi; Kojima, Seiji.

In: Leukemia Research, Vol. 32, No. 7, 01.07.2008, p. 1036-1042.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay

AU - Tanaka, Makito

AU - Takahashi, Yoshiyuki

AU - Xu, Yin Yan

AU - Yoshida, Nao

AU - Yoshimi, Ayami

AU - Villalobos, Itzel Bustos

AU - Hama, Asahito

AU - Nishio, Nobuhiro

AU - Hidaka, Hirokazu

AU - Wang, Yue

AU - Yagasaki, Hiroshi

AU - Kojima, Seiji

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AB - Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

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Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay

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