Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay

Makito Tanaka; Yoshiyuki Takahashi; Yin Yan Xu; Nao Yoshida; Ayami Yoshimi; Itzel Bustos Villalobos; Asahito Hama; Nobuhiro Nishio; Hirokazu Hidaka; Yue Wang; Hiroshi Yagasaki; Seiji Kojima

doi:10.1016/j.leukres.2007.11.016

Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay

Makito Tanaka, Yoshiyuki Takahashi, Yin Yan Xu, Nao Yoshida, Ayami Yoshimi, Itzel Bustos Villalobos, Asahito Hama, Nobuhiro Nishio, Hirokazu Hidaka, Yue Wang, Hiroshi Yagasaki, Seiji Kojima

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a ³H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

Original language	English
Pages (from-to)	1036-1042
Number of pages	7
Journal	Leukemia Research
Volume	32
Issue number	7
DOIs	https://doi.org/10.1016/j.leukres.2007.11.016
Publication status	Published - 07-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Hematology
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.leukres.2007.11.016

Cite this

Tanaka, M., Takahashi, Y., Xu, Y. Y., Yoshida, N., Yoshimi, A., Villalobos, I. B., Hama, A., Nishio, N., Hidaka, H., Wang, Y., Yagasaki, H., & Kojima, S. (2008). Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay. Leukemia Research, 32(7), 1036-1042. https://doi.org/10.1016/j.leukres.2007.11.016

@article{a6e42956767544e49800dc37b35219ea,

title = "Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay",

abstract = "Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.",

keywords = "Colony-forming unit granulocyte-macrophage (CFU-GM), Granulocyte-macrophage colony-stimulating factor (GM-CSF), H-thymidine assay, Hypersensitivity, Juvenile myelomonocytic leukemia (JMML), PTPN11 mutation, RAS mutation",

author = "Makito Tanaka and Yoshiyuki Takahashi and Xu, {Yin Yan} and Nao Yoshida and Ayami Yoshimi and Villalobos, {Itzel Bustos} and Asahito Hama and Nobuhiro Nishio and Hirokazu Hidaka and Yue Wang and Hiroshi Yagasaki and Seiji Kojima",

year = "2008",

month = jul,

doi = "10.1016/j.leukres.2007.11.016",

language = "English",

volume = "32",

pages = "1036--1042",

journal = "Leukemia Research",

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Tanaka, M, Takahashi, Y, Xu, YY, Yoshida, N, Yoshimi, A, Villalobos, IB, Hama, A, Nishio, N, Hidaka, H, Wang, Y, Yagasaki, H & Kojima, S 2008, 'Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by ³H-thymidine assay', Leukemia Research, vol. 32, no. 7, pp. 1036-1042. https://doi.org/10.1016/j.leukres.2007.11.016

TY - JOUR

T1 - Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay

AU - Tanaka, Makito

AU - Takahashi, Yoshiyuki

AU - Xu, Yin Yan

AU - Yoshida, Nao

AU - Yoshimi, Ayami

AU - Villalobos, Itzel Bustos

AU - Hama, Asahito

AU - Nishio, Nobuhiro

AU - Hidaka, Hirokazu

AU - Wang, Yue

AU - Yagasaki, Hiroshi

AU - Kojima, Seiji

PY - 2008/7

Y1 - 2008/7

N2 - Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

AB - Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

KW - Colony-forming unit granulocyte-macrophage (CFU-GM)

KW - Granulocyte-macrophage colony-stimulating factor (GM-CSF)

KW - H-thymidine assay

KW - Hypersensitivity

KW - Juvenile myelomonocytic leukemia (JMML)

KW - PTPN11 mutation

KW - RAS mutation

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