Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay

Makito Tanaka, Yoshiyuki Takahashi, Yin Yan Xu, Nao Yoshida, Ayami Yoshimi, Itzel Bustos Villalobos, Asahito Hama, Nobuhiro Nishio, Hirokazu Hidaka, Yue Wang, Hiroshi Yagasaki, Seiji Kojima

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

Original languageEnglish
Pages (from-to)1036-1042
Number of pages7
JournalLeukemia Research
Volume32
Issue number7
DOIs
Publication statusPublished - 01-07-2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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