Abstract
Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a 3H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.
Original language | English |
---|---|
Pages (from-to) | 1036-1042 |
Number of pages | 7 |
Journal | Leukemia Research |
Volume | 32 |
Issue number | 7 |
DOIs | |
Publication status | Published - 07-2008 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Hematology
- Oncology
- Cancer Research