Quasispecies nature of hepatitis C virus (HCV) in patients with chronic hepatitis C with mixed HCV subtypes

Hidenori Toyoda, Yoshihide Fukuda, Isao Nakano, Yoshiaki Katano, Tetsuo Takayama, Takashi Kumada, Satoshi Nakano, Junki Takamatsu, Hidehiko Saito, Tetsuo Hayakawa

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The quasispecies nature of hepatitis C virus (HCV) in patients with mixed HCV subtype infection was compared with that in patients with single HCV subtype infection. The number of HCV quasispecies was compared between 35 patients with mixed HCV subtype infection and 83 patients with single subtype infection. Subtype was determined by primers deduced from the core region and by line probe assay respectively. The number of quasispecies was evaluated by polymerase chain reaction amplification of hypervariable region 1 and by fluorescence single-strand conformation polymorphism analysis. There was no difference in clinical background between patients with mixed subtype infection and patients with single subtype infection. The number of quasispecies in patients with multiple subtype HCV infection was larger than in patients with single subtype HCV infection. The immunologic environments which allow the coexistence of more HCV quasispecies in patients with multiple HCV subtype infection differs from that in patients with single HCV subtype infection.

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalJournal of Medical Virology
Volume54
Issue number2
DOIs
Publication statusPublished - 02-1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

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