TY - JOUR
T1 - Quercetin protects against pulmonary oxidant stress via heme oxygenase-1 induction in lung epithelial cells
AU - Hayashi, Yuta
AU - Matsushima, Miyoko
AU - Nakamura, Toshinobu
AU - Shibasaki, Masataka
AU - Hashimoto, Naozumi
AU - Imaizumi, Kazuyoshi
AU - Shimokata, Kaoru
AU - Hasegawa, Yoshinori
AU - Kawabe, Tsutomu
PY - 2012/1/6
Y1 - 2012/1/6
N2 - The lung is a primary target for oxygen toxicity because of its constant exposure to high oxygen levels and environmental oxidants. Quercetin is one of the most commonly found dietary flavonoids, and it provides cytoprotective actions via activation of specific transcriptional factors and upregulation of endogenous defensive pathways. In the present study, we showed that quercetin increased the levels of heme oxygenase (HO)-1 expression and protected against hydrogen peroxide (H 2O 2)-induced cytotoxicity in lung epithelial cell lines. Quercetin suppressed H 2O 2-induced apoptotic events, including hypodiploid cells, activation of caspase 3 enzyme activity and lactate dehydrogenase release. This cytoprotective effect was attenuated by the addition of the HO inhibitor, tin protoporphyrin IX. In addition, the end products of heme metabolites catalyzed by HO-1, carbon monoxide and bilirubin, protect against H 2O 2-induced cytotoxicity in LA-4 cells. Quercetin may well be one of the promising substances to attenuate oxidative epithelial cell injury in lung inflammation.
AB - The lung is a primary target for oxygen toxicity because of its constant exposure to high oxygen levels and environmental oxidants. Quercetin is one of the most commonly found dietary flavonoids, and it provides cytoprotective actions via activation of specific transcriptional factors and upregulation of endogenous defensive pathways. In the present study, we showed that quercetin increased the levels of heme oxygenase (HO)-1 expression and protected against hydrogen peroxide (H 2O 2)-induced cytotoxicity in lung epithelial cell lines. Quercetin suppressed H 2O 2-induced apoptotic events, including hypodiploid cells, activation of caspase 3 enzyme activity and lactate dehydrogenase release. This cytoprotective effect was attenuated by the addition of the HO inhibitor, tin protoporphyrin IX. In addition, the end products of heme metabolites catalyzed by HO-1, carbon monoxide and bilirubin, protect against H 2O 2-induced cytotoxicity in LA-4 cells. Quercetin may well be one of the promising substances to attenuate oxidative epithelial cell injury in lung inflammation.
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U2 - 10.1016/j.bbrc.2011.11.078
DO - 10.1016/j.bbrc.2011.11.078
M3 - Article
C2 - 22138401
AN - SCOPUS:84855794519
VL - 417
SP - 169
EP - 174
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -