Quinolinic acid production is related to macrophage tropic isolates of HIV-1.

B. J. Brew, J. Corbeil, L. Pemberton, L. Evans, Kuniaki Saito, R. Penny, D. A. Cooper, M. P. Heyes

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We sought to determine whether the neurotoxin quinolinic acid (QUIN) was produced by macrophages or lymphocytes infected with isolates of HIV-1 with varying degrees of macrophage tropism derived from patients with varying stages of AIDS dementia complex (ADC). Highly macrophage tropic isolates and minimally macrophage tropic isolates were used to inoculate macrophages and QUIN production was measured. Similarly, QUIN production from macrophages was monitored using a purified cell free highly macrophage tropic isolate and laboratory isolates SF33 and SF2. Each of these experiments was also performed with lymphocytes. We found that macrophages infected with macrophage tropic isolates of HIV-1 led to QUIN production while lymphocytes did not produce QUIN. The ability of the HIV-1 infected macrophages to produce QUIN was related to the viral inoculum and the degree of macrophage tropism of the isolate. The severity of ADC in the patient from whom a particular isolate was derived was not per se a determining factor for QUIN production. Purified cell free ADC isolates also led to QUIN production by macrophages thereby suggesting that HIV-1 infection alone is capable of inducing QUIN production.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalJournal of NeuroVirology
Volume1
Issue number5-6
DOIs
Publication statusPublished - 01-01-1995
Externally publishedYes

Fingerprint

Quinolinic Acid
HIV-1
Macrophages
AIDS Dementia Complex
Tropism
Lymphocytes
Aptitude
Neurotoxins
HIV Infections

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Virology

Cite this

Brew, B. J., Corbeil, J., Pemberton, L., Evans, L., Saito, K., Penny, R., ... Heyes, M. P. (1995). Quinolinic acid production is related to macrophage tropic isolates of HIV-1. Journal of NeuroVirology, 1(5-6), 369-374. https://doi.org/10.3109/13550289509111026
Brew, B. J. ; Corbeil, J. ; Pemberton, L. ; Evans, L. ; Saito, Kuniaki ; Penny, R. ; Cooper, D. A. ; Heyes, M. P. / Quinolinic acid production is related to macrophage tropic isolates of HIV-1. In: Journal of NeuroVirology. 1995 ; Vol. 1, No. 5-6. pp. 369-374.
@article{b46dbad8d7c04ab68f145e499c3e6507,
title = "Quinolinic acid production is related to macrophage tropic isolates of HIV-1.",
abstract = "We sought to determine whether the neurotoxin quinolinic acid (QUIN) was produced by macrophages or lymphocytes infected with isolates of HIV-1 with varying degrees of macrophage tropism derived from patients with varying stages of AIDS dementia complex (ADC). Highly macrophage tropic isolates and minimally macrophage tropic isolates were used to inoculate macrophages and QUIN production was measured. Similarly, QUIN production from macrophages was monitored using a purified cell free highly macrophage tropic isolate and laboratory isolates SF33 and SF2. Each of these experiments was also performed with lymphocytes. We found that macrophages infected with macrophage tropic isolates of HIV-1 led to QUIN production while lymphocytes did not produce QUIN. The ability of the HIV-1 infected macrophages to produce QUIN was related to the viral inoculum and the degree of macrophage tropism of the isolate. The severity of ADC in the patient from whom a particular isolate was derived was not per se a determining factor for QUIN production. Purified cell free ADC isolates also led to QUIN production by macrophages thereby suggesting that HIV-1 infection alone is capable of inducing QUIN production.",
author = "Brew, {B. J.} and J. Corbeil and L. Pemberton and L. Evans and Kuniaki Saito and R. Penny and Cooper, {D. A.} and Heyes, {M. P.}",
year = "1995",
month = "1",
day = "1",
doi = "10.3109/13550289509111026",
language = "English",
volume = "1",
pages = "369--374",
journal = "Journal of NeuroVirology",
issn = "1355-0284",
publisher = "Springer New York",
number = "5-6",

}

Brew, BJ, Corbeil, J, Pemberton, L, Evans, L, Saito, K, Penny, R, Cooper, DA & Heyes, MP 1995, 'Quinolinic acid production is related to macrophage tropic isolates of HIV-1.', Journal of NeuroVirology, vol. 1, no. 5-6, pp. 369-374. https://doi.org/10.3109/13550289509111026

Quinolinic acid production is related to macrophage tropic isolates of HIV-1. / Brew, B. J.; Corbeil, J.; Pemberton, L.; Evans, L.; Saito, Kuniaki; Penny, R.; Cooper, D. A.; Heyes, M. P.

In: Journal of NeuroVirology, Vol. 1, No. 5-6, 01.01.1995, p. 369-374.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quinolinic acid production is related to macrophage tropic isolates of HIV-1.

AU - Brew, B. J.

AU - Corbeil, J.

AU - Pemberton, L.

AU - Evans, L.

AU - Saito, Kuniaki

AU - Penny, R.

AU - Cooper, D. A.

AU - Heyes, M. P.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - We sought to determine whether the neurotoxin quinolinic acid (QUIN) was produced by macrophages or lymphocytes infected with isolates of HIV-1 with varying degrees of macrophage tropism derived from patients with varying stages of AIDS dementia complex (ADC). Highly macrophage tropic isolates and minimally macrophage tropic isolates were used to inoculate macrophages and QUIN production was measured. Similarly, QUIN production from macrophages was monitored using a purified cell free highly macrophage tropic isolate and laboratory isolates SF33 and SF2. Each of these experiments was also performed with lymphocytes. We found that macrophages infected with macrophage tropic isolates of HIV-1 led to QUIN production while lymphocytes did not produce QUIN. The ability of the HIV-1 infected macrophages to produce QUIN was related to the viral inoculum and the degree of macrophage tropism of the isolate. The severity of ADC in the patient from whom a particular isolate was derived was not per se a determining factor for QUIN production. Purified cell free ADC isolates also led to QUIN production by macrophages thereby suggesting that HIV-1 infection alone is capable of inducing QUIN production.

AB - We sought to determine whether the neurotoxin quinolinic acid (QUIN) was produced by macrophages or lymphocytes infected with isolates of HIV-1 with varying degrees of macrophage tropism derived from patients with varying stages of AIDS dementia complex (ADC). Highly macrophage tropic isolates and minimally macrophage tropic isolates were used to inoculate macrophages and QUIN production was measured. Similarly, QUIN production from macrophages was monitored using a purified cell free highly macrophage tropic isolate and laboratory isolates SF33 and SF2. Each of these experiments was also performed with lymphocytes. We found that macrophages infected with macrophage tropic isolates of HIV-1 led to QUIN production while lymphocytes did not produce QUIN. The ability of the HIV-1 infected macrophages to produce QUIN was related to the viral inoculum and the degree of macrophage tropism of the isolate. The severity of ADC in the patient from whom a particular isolate was derived was not per se a determining factor for QUIN production. Purified cell free ADC isolates also led to QUIN production by macrophages thereby suggesting that HIV-1 infection alone is capable of inducing QUIN production.

UR - http://www.scopus.com/inward/record.url?scp=0029420019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029420019&partnerID=8YFLogxK

U2 - 10.3109/13550289509111026

DO - 10.3109/13550289509111026

M3 - Article

VL - 1

SP - 369

EP - 374

JO - Journal of NeuroVirology

JF - Journal of NeuroVirology

SN - 1355-0284

IS - 5-6

ER -