Quinolone resistance mutations in Klebsiella pneumoniae

Shinichi Ando, Tomomi Katayama, Satoshi Hayakawa, Kiyohito Ishikawa, Masaki Horiba, Masanori Yanaoka, Kiyotaka Hoshinaga, Keizo Suzuki, Mayumi Shonai, Hiroaki Yoshida, Shinichi Nakamura, Yorio Naide

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Single-strand conformational polymorphism (SSCP) analysis of 20 quinolone-resistant clinical isolates of Klebsiella pneumoniae showed five mobility patterns. DNA sequence analysis of these fragments revealed that DNA fragments with a mutation (s) in the gyrA gene showed different patterns from the pattern of the wild type gyrA fragment and that one mutation group yielded the specific mobility pattern, which enabled us to anticipate the mutation (s) in the gyrA gene by SSCP analysis. When transformed with the wild type Escherichia coli gyrA gene, the mutants tested became quinolone susceptible, suggesting that the gyrA mutations are responsible for quinolone resistance.

Original languageEnglish
Pages (from-to)673-675
Number of pages3
JournalJapanese Journal of Chemotherapy
Volume45
Issue number8
Publication statusPublished - 01-12-1997

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

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