Quinolone resistance mutations in Klebsiella pneumoniae

Shinichi Ando; Tomomi Katayama; Satoshi Hayakawa; Kiyohito Ishikawa; Masaki Horiba; Masanori Yanaoka; Kiyotaka Hoshinaga; Keizo Suzuki; Mayumi Shonai; Hiroaki Yoshida; Shinichi Nakamura; Yorio Naide

Quinolone resistance mutations in Klebsiella pneumoniae

Shinichi Ando, Tomomi Katayama, Satoshi Hayakawa, Kiyohito Ishikawa, Masaki Horiba, Masanori Yanaoka, Kiyotaka Hoshinaga, Keizo Suzuki, Mayumi Shonai, Hiroaki Yoshida, Shinichi Nakamura, Yorio Naide

Quality and Safety in Healthcare

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Abstract

Single-strand conformational polymorphism (SSCP) analysis of 20 quinolone-resistant clinical isolates of Klebsiella pneumoniae showed five mobility patterns. DNA sequence analysis of these fragments revealed that DNA fragments with a mutation (s) in the gyrA gene showed different patterns from the pattern of the wild type gyrA fragment and that one mutation group yielded the specific mobility pattern, which enabled us to anticipate the mutation (s) in the gyrA gene by SSCP analysis. When transformed with the wild type Escherichia coli gyrA gene, the mutants tested became quinolone susceptible, suggesting that the gyrA mutations are responsible for quinolone resistance.

Original language	English
Pages (from-to)	673-675
Number of pages	3
Journal	Japanese Journal of Chemotherapy
Volume	45
Issue number	8
Publication status	Published - 1997

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmacology (medical)

Cite this

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title = "Quinolone resistance mutations in Klebsiella pneumoniae",

abstract = "Single-strand conformational polymorphism (SSCP) analysis of 20 quinolone-resistant clinical isolates of Klebsiella pneumoniae showed five mobility patterns. DNA sequence analysis of these fragments revealed that DNA fragments with a mutation (s) in the gyrA gene showed different patterns from the pattern of the wild type gyrA fragment and that one mutation group yielded the specific mobility pattern, which enabled us to anticipate the mutation (s) in the gyrA gene by SSCP analysis. When transformed with the wild type Escherichia coli gyrA gene, the mutants tested became quinolone susceptible, suggesting that the gyrA mutations are responsible for quinolone resistance.",

author = "Shinichi Ando and Tomomi Katayama and Satoshi Hayakawa and Kiyohito Ishikawa and Masaki Horiba and Masanori Yanaoka and Kiyotaka Hoshinaga and Keizo Suzuki and Mayumi Shonai and Hiroaki Yoshida and Shinichi Nakamura and Yorio Naide",

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T1 - Quinolone resistance mutations in Klebsiella pneumoniae

AU - Ando, Shinichi

AU - Katayama, Tomomi

AU - Hayakawa, Satoshi

AU - Ishikawa, Kiyohito

AU - Horiba, Masaki

AU - Yanaoka, Masanori

AU - Hoshinaga, Kiyotaka

AU - Suzuki, Keizo

AU - Shonai, Mayumi

AU - Yoshida, Hiroaki

AU - Nakamura, Shinichi

AU - Naide, Yorio

PY - 1997

Y1 - 1997

N2 - Single-strand conformational polymorphism (SSCP) analysis of 20 quinolone-resistant clinical isolates of Klebsiella pneumoniae showed five mobility patterns. DNA sequence analysis of these fragments revealed that DNA fragments with a mutation (s) in the gyrA gene showed different patterns from the pattern of the wild type gyrA fragment and that one mutation group yielded the specific mobility pattern, which enabled us to anticipate the mutation (s) in the gyrA gene by SSCP analysis. When transformed with the wild type Escherichia coli gyrA gene, the mutants tested became quinolone susceptible, suggesting that the gyrA mutations are responsible for quinolone resistance.

AB - Single-strand conformational polymorphism (SSCP) analysis of 20 quinolone-resistant clinical isolates of Klebsiella pneumoniae showed five mobility patterns. DNA sequence analysis of these fragments revealed that DNA fragments with a mutation (s) in the gyrA gene showed different patterns from the pattern of the wild type gyrA fragment and that one mutation group yielded the specific mobility pattern, which enabled us to anticipate the mutation (s) in the gyrA gene by SSCP analysis. When transformed with the wild type Escherichia coli gyrA gene, the mutants tested became quinolone susceptible, suggesting that the gyrA mutations are responsible for quinolone resistance.

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M3 - Article

AN - SCOPUS:0030884674

SN - 1340-7007

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JO - Japanese Journal of Chemotherapy

JF - Japanese Journal of Chemotherapy

IS - 8

ER -

Quinolone resistance mutations in Klebsiella pneumoniae

Abstract

All Science Journal Classification (ASJC) codes

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