Rac1 is required for the formation of three germ layers during gastrulation

Kazuhiro Sugihara; Norio Nakatsuji; Kenji Nakamura; Kazuki Nakao; Ryuju Hashimoto; Hiroki Otani; Hiroyuki Sakagami; Hisatake Kondo; Shiro Nozawa; Atsu Aiba; Motoya Katsuki

doi:10.1038/sj.onc.1202595

Rac1 is required for the formation of three germ layers during gastrulation

Kazuhiro Sugihara, Norio Nakatsuji, Kenji Nakamura, Kazuki Nakao, Ryuju Hashimoto, Hiroki Otani, Hiroyuki Sakagami, Hisatake Kondo, Shiro Nozawa, Atsu Aiba, Motoya Katsuki

Research output: Contribution to journal › Article › peer-review

285 Citations (Scopus)

Abstract

The Rac1, a member of the Rho family proteins, regulates actin organization of cytoskeleton and cell adhesion. We used genetic analysis to elucidate the role of Rac1 in mouse embryonic development. The rac1 deficient embryos showed numerous cell deaths in the space between the embryonic ectoderm and endoderm at the primitive streak stage. Investigation of the primary epiblast culture isolated from rac1 deficient embryos indicated that Rac1 is involved in lamellipodia formation, cell adhesion and cell migration in vivo. These results suggest that Rac1-mediated cell adhesion is essential for the formation of three germ layers during gastrulation.

Original language	English
Pages (from-to)	3427-3433
Number of pages	7
Journal	Oncogene
Volume	17
Issue number	26
DOIs	https://doi.org/10.1038/sj.onc.1202595
Publication status	Published - 31-12-1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/sj.onc.1202595

Cite this

@article{8af2a3523f9b4eb1aeae18a2b7766b57,

title = "Rac1 is required for the formation of three germ layers during gastrulation",

abstract = "The Rac1, a member of the Rho family proteins, regulates actin organization of cytoskeleton and cell adhesion. We used genetic analysis to elucidate the role of Rac1 in mouse embryonic development. The rac1 deficient embryos showed numerous cell deaths in the space between the embryonic ectoderm and endoderm at the primitive streak stage. Investigation of the primary epiblast culture isolated from rac1 deficient embryos indicated that Rac1 is involved in lamellipodia formation, cell adhesion and cell migration in vivo. These results suggest that Rac1-mediated cell adhesion is essential for the formation of three germ layers during gastrulation.",

author = "Kazuhiro Sugihara and Norio Nakatsuji and Kenji Nakamura and Kazuki Nakao and Ryuju Hashimoto and Hiroki Otani and Hiroyuki Sakagami and Hisatake Kondo and Shiro Nozawa and Atsu Aiba and Motoya Katsuki",

note = "Funding Information: We thank Yoshimi Takai for the human rac1 cDNA; Elizabeth J Robertson for the CCE ES cells; Kuniko Katsuki for her excellent technical assistance; Naomi Nakagata for help with the morphological analyses; Toshihisa Hatta, and Yumiko Takeda for help with the histological analyses; Kenji Takaishi for helpful discussion. This work was supported in part by Grants-in-Aid for Scientific Research on Priority Area, for Cancer Research and Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan, and Grants-in-Aid from the Ministry of Health and Welfare, Japan.",

year = "1998",

month = dec,

day = "31",

doi = "10.1038/sj.onc.1202595",

language = "English",

volume = "17",

pages = "3427--3433",

journal = "Oncogene",

issn = "0950-9232",

publisher = "Springer Nature",

number = "26",

}

TY - JOUR

T1 - Rac1 is required for the formation of three germ layers during gastrulation

AU - Sugihara, Kazuhiro

AU - Nakatsuji, Norio

AU - Nakamura, Kenji

AU - Nakao, Kazuki

AU - Hashimoto, Ryuju

AU - Otani, Hiroki

AU - Sakagami, Hiroyuki

AU - Kondo, Hisatake

AU - Nozawa, Shiro

AU - Aiba, Atsu

AU - Katsuki, Motoya

N1 - Funding Information: We thank Yoshimi Takai for the human rac1 cDNA; Elizabeth J Robertson for the CCE ES cells; Kuniko Katsuki for her excellent technical assistance; Naomi Nakagata for help with the morphological analyses; Toshihisa Hatta, and Yumiko Takeda for help with the histological analyses; Kenji Takaishi for helpful discussion. This work was supported in part by Grants-in-Aid for Scientific Research on Priority Area, for Cancer Research and Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan, and Grants-in-Aid from the Ministry of Health and Welfare, Japan.

PY - 1998/12/31

Y1 - 1998/12/31

N2 - The Rac1, a member of the Rho family proteins, regulates actin organization of cytoskeleton and cell adhesion. We used genetic analysis to elucidate the role of Rac1 in mouse embryonic development. The rac1 deficient embryos showed numerous cell deaths in the space between the embryonic ectoderm and endoderm at the primitive streak stage. Investigation of the primary epiblast culture isolated from rac1 deficient embryos indicated that Rac1 is involved in lamellipodia formation, cell adhesion and cell migration in vivo. These results suggest that Rac1-mediated cell adhesion is essential for the formation of three germ layers during gastrulation.

AB - The Rac1, a member of the Rho family proteins, regulates actin organization of cytoskeleton and cell adhesion. We used genetic analysis to elucidate the role of Rac1 in mouse embryonic development. The rac1 deficient embryos showed numerous cell deaths in the space between the embryonic ectoderm and endoderm at the primitive streak stage. Investigation of the primary epiblast culture isolated from rac1 deficient embryos indicated that Rac1 is involved in lamellipodia formation, cell adhesion and cell migration in vivo. These results suggest that Rac1-mediated cell adhesion is essential for the formation of three germ layers during gastrulation.

UR - http://www.scopus.com/inward/record.url?scp=0032585635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032585635&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1202595

DO - 10.1038/sj.onc.1202595

M3 - Article

C2 - 10030666

AN - SCOPUS:0032585635

SN - 0950-9232

VL - 17

SP - 3427

EP - 3433

JO - Oncogene

JF - Oncogene

IS - 26

ER -

Rac1 is required for the formation of three germ layers during gastrulation

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this