Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method

Seiko Iwata, Yukiko Shibata, Jun ichi Kawada, Shinya Hara, Yukihiro Nishiyama, Tsuneo Morishima, Masaru Ihira, Tetsushi Yoshikawa, Yoshizo Asano, Hiroshi Kimura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: The loop-mediated isothermal amplification (LAMP) method is a novel technique for the amplification of specific DNA sequences. Objectives: To establish the LAMP method for amplifying Epstein-Barr virus (EBV) DNA and to examine its reliability for the detection of EBV DNA in clinical specimens. Study design: Sera from 108 patients, who were initially suspected of primary EBV infection, were tested by the EBV LAMP method, and the results were compared with those of the real-time PCR assay. Serological examination was regarded as the standard diagnostic method. Results: To diagnose primary EBV infection, the sensitivity of LAMP was 86.4% and the specificity was 100%. The sensitivity of the real-time PCR assay was 84.1% and the specificity was 98.4%. Longitudinal analysis showed that the detection rate of EBV DNA in serum by the LAMP method decreased with time in accordance with the decrease of the EBV load. EBV DNA could not be detected in serum 40 days after onset of symptoms. Conclusions: These results indicate that the sensitivity and specificity of the LAMP method are comparable to those of real-time PCR and that detecting EBV DNA in serum by this method is potentially useful for diagnosing primary EBV infection.

Original languageEnglish
Pages (from-to)128-133
Number of pages6
JournalJournal of Clinical Virology
Volume37
Issue number2
DOIs
Publication statusPublished - 01-10-2006

Fingerprint

Human Herpesvirus 4
DNA
Epstein-Barr Virus Infections
Real-Time Polymerase Chain Reaction
Serum
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Iwata, S., Shibata, Y., Kawada, J. I., Hara, S., Nishiyama, Y., Morishima, T., ... Kimura, H. (2006). Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method. Journal of Clinical Virology, 37(2), 128-133. https://doi.org/10.1016/j.jcv.2006.07.011
Iwata, Seiko ; Shibata, Yukiko ; Kawada, Jun ichi ; Hara, Shinya ; Nishiyama, Yukihiro ; Morishima, Tsuneo ; Ihira, Masaru ; Yoshikawa, Tetsushi ; Asano, Yoshizo ; Kimura, Hiroshi. / Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method. In: Journal of Clinical Virology. 2006 ; Vol. 37, No. 2. pp. 128-133.
@article{10796370163f4bc9a0ad0cb721ff8860,
title = "Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method",
abstract = "Background: The loop-mediated isothermal amplification (LAMP) method is a novel technique for the amplification of specific DNA sequences. Objectives: To establish the LAMP method for amplifying Epstein-Barr virus (EBV) DNA and to examine its reliability for the detection of EBV DNA in clinical specimens. Study design: Sera from 108 patients, who were initially suspected of primary EBV infection, were tested by the EBV LAMP method, and the results were compared with those of the real-time PCR assay. Serological examination was regarded as the standard diagnostic method. Results: To diagnose primary EBV infection, the sensitivity of LAMP was 86.4{\%} and the specificity was 100{\%}. The sensitivity of the real-time PCR assay was 84.1{\%} and the specificity was 98.4{\%}. Longitudinal analysis showed that the detection rate of EBV DNA in serum by the LAMP method decreased with time in accordance with the decrease of the EBV load. EBV DNA could not be detected in serum 40 days after onset of symptoms. Conclusions: These results indicate that the sensitivity and specificity of the LAMP method are comparable to those of real-time PCR and that detecting EBV DNA in serum by this method is potentially useful for diagnosing primary EBV infection.",
author = "Seiko Iwata and Yukiko Shibata and Kawada, {Jun ichi} and Shinya Hara and Yukihiro Nishiyama and Tsuneo Morishima and Masaru Ihira and Tetsushi Yoshikawa and Yoshizo Asano and Hiroshi Kimura",
year = "2006",
month = "10",
day = "1",
doi = "10.1016/j.jcv.2006.07.011",
language = "English",
volume = "37",
pages = "128--133",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",
number = "2",

}

Iwata, S, Shibata, Y, Kawada, JI, Hara, S, Nishiyama, Y, Morishima, T, Ihira, M, Yoshikawa, T, Asano, Y & Kimura, H 2006, 'Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method', Journal of Clinical Virology, vol. 37, no. 2, pp. 128-133. https://doi.org/10.1016/j.jcv.2006.07.011

Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method. / Iwata, Seiko; Shibata, Yukiko; Kawada, Jun ichi; Hara, Shinya; Nishiyama, Yukihiro; Morishima, Tsuneo; Ihira, Masaru; Yoshikawa, Tetsushi; Asano, Yoshizo; Kimura, Hiroshi.

In: Journal of Clinical Virology, Vol. 37, No. 2, 01.10.2006, p. 128-133.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method

AU - Iwata, Seiko

AU - Shibata, Yukiko

AU - Kawada, Jun ichi

AU - Hara, Shinya

AU - Nishiyama, Yukihiro

AU - Morishima, Tsuneo

AU - Ihira, Masaru

AU - Yoshikawa, Tetsushi

AU - Asano, Yoshizo

AU - Kimura, Hiroshi

PY - 2006/10/1

Y1 - 2006/10/1

N2 - Background: The loop-mediated isothermal amplification (LAMP) method is a novel technique for the amplification of specific DNA sequences. Objectives: To establish the LAMP method for amplifying Epstein-Barr virus (EBV) DNA and to examine its reliability for the detection of EBV DNA in clinical specimens. Study design: Sera from 108 patients, who were initially suspected of primary EBV infection, were tested by the EBV LAMP method, and the results were compared with those of the real-time PCR assay. Serological examination was regarded as the standard diagnostic method. Results: To diagnose primary EBV infection, the sensitivity of LAMP was 86.4% and the specificity was 100%. The sensitivity of the real-time PCR assay was 84.1% and the specificity was 98.4%. Longitudinal analysis showed that the detection rate of EBV DNA in serum by the LAMP method decreased with time in accordance with the decrease of the EBV load. EBV DNA could not be detected in serum 40 days after onset of symptoms. Conclusions: These results indicate that the sensitivity and specificity of the LAMP method are comparable to those of real-time PCR and that detecting EBV DNA in serum by this method is potentially useful for diagnosing primary EBV infection.

AB - Background: The loop-mediated isothermal amplification (LAMP) method is a novel technique for the amplification of specific DNA sequences. Objectives: To establish the LAMP method for amplifying Epstein-Barr virus (EBV) DNA and to examine its reliability for the detection of EBV DNA in clinical specimens. Study design: Sera from 108 patients, who were initially suspected of primary EBV infection, were tested by the EBV LAMP method, and the results were compared with those of the real-time PCR assay. Serological examination was regarded as the standard diagnostic method. Results: To diagnose primary EBV infection, the sensitivity of LAMP was 86.4% and the specificity was 100%. The sensitivity of the real-time PCR assay was 84.1% and the specificity was 98.4%. Longitudinal analysis showed that the detection rate of EBV DNA in serum by the LAMP method decreased with time in accordance with the decrease of the EBV load. EBV DNA could not be detected in serum 40 days after onset of symptoms. Conclusions: These results indicate that the sensitivity and specificity of the LAMP method are comparable to those of real-time PCR and that detecting EBV DNA in serum by this method is potentially useful for diagnosing primary EBV infection.

UR - http://www.scopus.com/inward/record.url?scp=33748954455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748954455&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2006.07.011

DO - 10.1016/j.jcv.2006.07.011

M3 - Article

C2 - 16973412

AN - SCOPUS:33748954455

VL - 37

SP - 128

EP - 133

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 2

ER -