Rapid detection of herpes simplex virus DNA in cerebrospinal fluid

Comparison between loop-mediated isothermal amplification and real-time PCR

Hiroshi Kimura, Masaru Ihira, Yoshihiro Enomoto, Jun Ichi Kawada, Yoshinori Ito, Tsuneo Morishima, Tetsushi Yoshikawa, Yoshizo Asano

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for diagnosing central nervous system infection with herpes simplex virus (HSV), we compared the LAMP method with real-time PCR, using samples that were previously tested by nested PCR. We examined 69 cerebrospinal fluid (CSF) samples from patients suspected of having HSV infection of the central nervous system. The results of the real-time PCR analysis and nested PCR assay were in complete accord. When nested PCR was regarded as standard, the sensitivity of LAMP was 81%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 90%. Although further improvement is necessary for the wide spread use, the LAMP method might be applicable to diagnosis of HSV infection of the central nervous system.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalMedical Microbiology and Immunology
Volume194
Issue number4
DOIs
Publication statusPublished - 01-08-2005

Fingerprint

Simplexvirus
Cerebrospinal Fluid
Real-Time Polymerase Chain Reaction
Virus Diseases
Polymerase Chain Reaction
DNA
Central Nervous System
Central Nervous System Infections
Nucleic Acids

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

Cite this

Kimura, Hiroshi ; Ihira, Masaru ; Enomoto, Yoshihiro ; Kawada, Jun Ichi ; Ito, Yoshinori ; Morishima, Tsuneo ; Yoshikawa, Tetsushi ; Asano, Yoshizo. / Rapid detection of herpes simplex virus DNA in cerebrospinal fluid : Comparison between loop-mediated isothermal amplification and real-time PCR. In: Medical Microbiology and Immunology. 2005 ; Vol. 194, No. 4. pp. 181-185.
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Rapid detection of herpes simplex virus DNA in cerebrospinal fluid : Comparison between loop-mediated isothermal amplification and real-time PCR. / Kimura, Hiroshi; Ihira, Masaru; Enomoto, Yoshihiro; Kawada, Jun Ichi; Ito, Yoshinori; Morishima, Tsuneo; Yoshikawa, Tetsushi; Asano, Yoshizo.

In: Medical Microbiology and Immunology, Vol. 194, No. 4, 01.08.2005, p. 181-185.

Research output: Contribution to journalArticle

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