Rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method

Yoshihiko Enomoto, Tetsushi Yoshikawa, Masaru Ihira, Shiho Akimoto, Fumi Miyake, Chie Usui, Sadao Suga, Kayoko Suzuki, Takashi Kawana, Yukihiro Nishiyama, Yoshizo Asano

Research output: Contribution to journalArticle

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Abstract

Primers for herpes simplex virus type 1 (HSV 1)-specific loop-mediated isothermal amplification (LAMP) method amplified HSV-1 DNA, while HSV-2-specific primers amplified only HSV-2 DNA; no LAMP products were produced by reactions performed with other viral DNAs. The sensitivities of the HSV-1- and HSV-2-specific LAMP methods, determined by agarose gel electrophoresis, reached 500 and 1,000 copies/tube, respectively. The turbidity assay, however, determined the sensitivity of the HSV-1- and HSV-2-specific LAMP methods to be 1,000 and 10,000 copies/tube, respectively. After initial validation studies, 18 swab samples (in sterilized water) collected from patients with either gingivostomatitis or vesicular skin eruptions were examined. HSV-1 LAMP products were detected by agarose gel electrophoresis in the 10 samples that also demonstrated viral DNA detection by real-time PCR. Nine of these 10 samples exhibited HSV-1 LAMP products by turbidity assay. Furthermore, both the agarose gel electrophoresis and the turbidity assay directly detected HSV-1 LAMP products in 9 of the 10 swab samples collected in sterilized water. Next, we examined the reliability of HSV type-specific LAMP for the detection of viral DNA in clinical specimens (culture medium) collected from genital lesions. HSV-2 was isolated from all of the samples and visualized by either agarose gel electrophoresis or turbidity assay.

Original languageEnglish
Pages (from-to)951-955
Number of pages5
JournalJournal of clinical microbiology
Volume43
Issue number2
DOIs
Publication statusPublished - 01-02-2005

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Human Herpesvirus 1
Virus Diseases
Simplexvirus
Human Herpesvirus 2
Agar Gel Electrophoresis
Viral DNA
Water
Validation Studies
DNA
Culture Media
Real-Time Polymerase Chain Reaction
Skin

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Enomoto, Yoshihiko ; Yoshikawa, Tetsushi ; Ihira, Masaru ; Akimoto, Shiho ; Miyake, Fumi ; Usui, Chie ; Suga, Sadao ; Suzuki, Kayoko ; Kawana, Takashi ; Nishiyama, Yukihiro ; Asano, Yoshizo. / Rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method. In: Journal of clinical microbiology. 2005 ; Vol. 43, No. 2. pp. 951-955.
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Enomoto, Y, Yoshikawa, T, Ihira, M, Akimoto, S, Miyake, F, Usui, C, Suga, S, Suzuki, K, Kawana, T, Nishiyama, Y & Asano, Y 2005, 'Rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method', Journal of clinical microbiology, vol. 43, no. 2, pp. 951-955. https://doi.org/10.1128/JCM.43.2.951-955.2005

Rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method. / Enomoto, Yoshihiko; Yoshikawa, Tetsushi; Ihira, Masaru; Akimoto, Shiho; Miyake, Fumi; Usui, Chie; Suga, Sadao; Suzuki, Kayoko; Kawana, Takashi; Nishiyama, Yukihiro; Asano, Yoshizo.

In: Journal of clinical microbiology, Vol. 43, No. 2, 01.02.2005, p. 951-955.

Research output: Contribution to journalArticle

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AU - Enomoto, Yoshihiko

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AU - Ihira, Masaru

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AU - Miyake, Fumi

AU - Usui, Chie

AU - Suga, Sadao

AU - Suzuki, Kayoko

AU - Kawana, Takashi

AU - Nishiyama, Yukihiro

AU - Asano, Yoshizo

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