Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green

Yoshiaki Shinden; Hiroki Ueo; Taro Tobo; Ayako Gamachi; Mitsuaki Utou; Hisateru Komatsu; Sho Nambara; Tomoko Saito; Masami Ueda; Hidenari Hirata; Shotaro Sakimura; Yuki Takano; Ryutaro Uchi; Junji Kurashige; Sayuri Akiyoshi; Tomohiro Iguchi; Hidetoshi Eguchi; Keishi Sugimachi; Yoko Kubota; Yuichiro Kai; Kenji Shibuta; Yuko Kijima; Heiji Yoshinaka; Shoji Natsugoe; Masaki Mori; Yoshihiko Maehara; Masayo Sakabe; Mako Kamiya; John W. Kakareka; Thomas J. Pohida; Peter L. Choyke; Hisataka Kobayashi; Hiroaki Ueo; Yasuteru Urano; Koshi Mimori

doi:10.1038/srep27525

Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green

Yoshiaki Shinden, Hiroki Ueo, Taro Tobo, Ayako Gamachi, Mitsuaki Utou, Hisateru Komatsu, Sho Nambara, Tomoko Saito, Masami Ueda, Hidenari Hirata, Shotaro Sakimura, Yuki Takano, Ryutaro Uchi, Junji Kurashige, Sayuri Akiyoshi, Tomohiro Iguchi, Hidetoshi Eguchi, Keishi Sugimachi, Yoko Kubota, Yuichiro KaiKenji Shibuta, Yuko Kijima, Heiji Yoshinaka, Shoji Natsugoe, Masaki Mori, Yoshihiko Maehara, Masayo Sakabe, Mako Kamiya, John W. Kakareka, Thomas J. Pohida, Peter L. Choyke, Hisataka Kobayashi, Hiroaki Ueo, Yasuteru Urano, Koshi Mimori

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.

Original language	English
Article number	27525
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep27525
Publication status	Published - 09-06-2016
Externally published	Yes

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10.1038/srep27525

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Shinden, Y., Ueo, H., Tobo, T., Gamachi, A., Utou, M., Komatsu, H., Nambara, S., Saito, T., Ueda, M., Hirata, H., Sakimura, S., Takano, Y., Uchi, R., Kurashige, J., Akiyoshi, S., Iguchi, T., Eguchi, H., Sugimachi, K., Kubota, Y., ... Mimori, K. (2016). Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green. Scientific reports, 6, Article 27525. https://doi.org/10.1038/srep27525

@article{01ad7695bbee4d1092ca497f1e625166,

title = "Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green",

abstract = "Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.",

author = "Yoshiaki Shinden and Hiroki Ueo and Taro Tobo and Ayako Gamachi and Mitsuaki Utou and Hisateru Komatsu and Sho Nambara and Tomoko Saito and Masami Ueda and Hidenari Hirata and Shotaro Sakimura and Yuki Takano and Ryutaro Uchi and Junji Kurashige and Sayuri Akiyoshi and Tomohiro Iguchi and Hidetoshi Eguchi and Keishi Sugimachi and Yoko Kubota and Yuichiro Kai and Kenji Shibuta and Yuko Kijima and Heiji Yoshinaka and Shoji Natsugoe and Masaki Mori and Yoshihiko Maehara and Masayo Sakabe and Mako Kamiya and Kakareka, {John W.} and Pohida, {Thomas J.} and Choyke, {Peter L.} and Hisataka Kobayashi and Hiroaki Ueo and Yasuteru Urano and Koshi Mimori",

year = "2016",

month = jun,

day = "9",

doi = "10.1038/srep27525",

language = "English",

volume = "6",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

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Shinden, Y, Ueo, H, Tobo, T, Gamachi, A, Utou, M, Komatsu, H, Nambara, S, Saito, T, Ueda, M, Hirata, H, Sakimura, S, Takano, Y, Uchi, R, Kurashige, J, Akiyoshi, S, Iguchi, T, Eguchi, H, Sugimachi, K, Kubota, Y, Kai, Y, Shibuta, K, Kijima, Y, Yoshinaka, H, Natsugoe, S, Mori, M, Maehara, Y, Sakabe, M, Kamiya, M, Kakareka, JW, Pohida, TJ, Choyke, PL, Kobayashi, H, Ueo, H, Urano, Y & Mimori, K 2016, 'Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green', Scientific reports, vol. 6, 27525. https://doi.org/10.1038/srep27525

TY - JOUR

T1 - Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green

AU - Shinden, Yoshiaki

AU - Ueo, Hiroki

AU - Tobo, Taro

AU - Gamachi, Ayako

AU - Utou, Mitsuaki

AU - Komatsu, Hisateru

AU - Nambara, Sho

AU - Saito, Tomoko

AU - Ueda, Masami

AU - Hirata, Hidenari

AU - Sakimura, Shotaro

AU - Takano, Yuki

AU - Uchi, Ryutaro

AU - Kurashige, Junji

AU - Akiyoshi, Sayuri

AU - Iguchi, Tomohiro

AU - Eguchi, Hidetoshi

AU - Sugimachi, Keishi

AU - Kubota, Yoko

AU - Kai, Yuichiro

AU - Shibuta, Kenji

AU - Kijima, Yuko

AU - Yoshinaka, Heiji

AU - Natsugoe, Shoji

AU - Mori, Masaki

AU - Maehara, Yoshihiko

AU - Sakabe, Masayo

AU - Kamiya, Mako

AU - Kakareka, John W.

AU - Pohida, Thomas J.

AU - Choyke, Peter L.

AU - Kobayashi, Hisataka

AU - Ueo, Hiroaki

AU - Urano, Yasuteru

AU - Mimori, Koshi

PY - 2016/6/9

Y1 - 2016/6/9

N2 - Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.

AB - Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.

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U2 - 10.1038/srep27525

DO - 10.1038/srep27525

M3 - Article

C2 - 27277343

AN - SCOPUS:84974589050

SN - 2045-2322

VL - 6

JO - Scientific reports

JF - Scientific reports

M1 - 27525

ER -

Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green

Abstract

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