Abstract
Aim: We aimed to develop a rapid, simple procedure and an algorithm for quantitative analysis and classification of the metastatic risk of gastrointestinal stromal tumours (GIST) for clinical use. Materials and Methods: Eighteen specimens from laparoscopic local gastrectomy were assessed by flow cytometry. We devised a new risk classification for GIST by combining flow cytometry parameters with tumour size and evaluated whether the combined parameters correlated with the modified Fletcher risk classification. Results: We found a significant correlation between clinical prognostic factors (mitotic count and Ki-67 labelling index) and the flow cytometry parameters DNA ploidy, DNA index and S-phase fraction. The combined parameters established from tumour size and the flow cytometry parameters showed a high correlation with the modified Fletcher risk classification (p=0.0064). Flow cytometry had to be performed for approximately 10 minutes to determine the metastatic risk. Conclusion: Rapid flow cytometry parameters can classify risk without the need for histological analysis.
Original language | English |
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Pages (from-to) | 131-136 |
Number of pages | 6 |
Journal | Anticancer research |
Volume | 41 |
Issue number | 1 |
DOIs | |
Publication status | Published - 01-2021 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research