Rapid flow cytometry of gastrointestinal stromal tumours closely matches the modified fletcher classification

KIYOAKI TANIGUCHI, AKANE SUZUKI, AKIKO SERIZAWA, SHO KOTAKE, SHUNICHI ITO, KAZUOMI SUZUKI, TAKUJI YAMADA, TAKEHARU NOGUCHI, KUNIHIKO AMANO, MASAHO OTA, YOSHIHIRO MURAGAKI, MASAKAZU YAMAMOTO

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Aim: We aimed to develop a rapid, simple procedure and an algorithm for quantitative analysis and classification of the metastatic risk of gastrointestinal stromal tumours (GIST) for clinical use. Materials and Methods: Eighteen specimens from laparoscopic local gastrectomy were assessed by flow cytometry. We devised a new risk classification for GIST by combining flow cytometry parameters with tumour size and evaluated whether the combined parameters correlated with the modified Fletcher risk classification. Results: We found a significant correlation between clinical prognostic factors (mitotic count and Ki-67 labelling index) and the flow cytometry parameters DNA ploidy, DNA index and S-phase fraction. The combined parameters established from tumour size and the flow cytometry parameters showed a high correlation with the modified Fletcher risk classification (p=0.0064). Flow cytometry had to be performed for approximately 10 minutes to determine the metastatic risk. Conclusion: Rapid flow cytometry parameters can classify risk without the need for histological analysis.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalAnticancer research
Volume41
Issue number1
DOIs
Publication statusPublished - 01-2021
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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