TY - JOUR
T1 - Rapid identification of Leishmania species from formalin-fixed biopsy samples by polymorphism-specific polymerase chain reaction
AU - Mimori, Tatsuyuki
AU - Sasaki, Ji ichiro
AU - Nakata, Motomi
AU - Gomez, Eduardo A.
AU - Uezato, Hiroshi
AU - Nonaka, Shigeo
AU - Hashiguchi, Yoshihisa
AU - Furuya, M. Masato
AU - Saya, Hideyuki
N1 - Funding Information:
This work received financial support from the Overseas Scientific Research Program of the Ministry of Education, Science and Culture, Japan. We thank Dr. G. Grimaldi Jr., Instituto Oswaldo Cruz, Rio de Janeiro, Brazil, for supplying us with the Leishmania reference strains, Dr. M. Nakao and Dr. N. Hayashi, Kumamoto University School of Medicine, Japan, for their technical suggestions, Dr. A.J. Tosi, Columbia University, NY, USA, for reading the manuscript and K. Uriuda for secretarial assistance.
PY - 1998/4/14
Y1 - 1998/4/14
N2 - The precise identification and classification of Leishmania species is important for public health surveillance since different species cause different clinical features of the disease. A highly specific polymerase chain reaction (PCR) panel was developed to enable the identification of the five major Leishmania species that cause New World cutaneous leishmaniases. The primers used for this panel were designed to distinguish the polymorphism in sequences of commonly amplified DNA bands of the parasites produced by arbitrarily primed PCR. These polymorphism-specific PCR diagnoses were performed with formalin-fixed biopsy specimens of the leishmanial lesions from four patients in Ecuador and one hamster skin lesion, and these lesions were determined to be caused by Leishmania (Viannia) panamensis, L. (Leishmania) mexicana, and L. (L.) amazonensis. The PCR panel may offer an important and practical approach to the standardized identification of Leishmania species in field examinations.
AB - The precise identification and classification of Leishmania species is important for public health surveillance since different species cause different clinical features of the disease. A highly specific polymerase chain reaction (PCR) panel was developed to enable the identification of the five major Leishmania species that cause New World cutaneous leishmaniases. The primers used for this panel were designed to distinguish the polymorphism in sequences of commonly amplified DNA bands of the parasites produced by arbitrarily primed PCR. These polymorphism-specific PCR diagnoses were performed with formalin-fixed biopsy specimens of the leishmanial lesions from four patients in Ecuador and one hamster skin lesion, and these lesions were determined to be caused by Leishmania (Viannia) panamensis, L. (Leishmania) mexicana, and L. (L.) amazonensis. The PCR panel may offer an important and practical approach to the standardized identification of Leishmania species in field examinations.
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U2 - 10.1016/S0378-1119(97)00663-X
DO - 10.1016/S0378-1119(97)00663-X
M3 - Article
C2 - 9573358
AN - SCOPUS:0032515944
SN - 0378-1119
VL - 210
SP - 179
EP - 186
JO - Gene
JF - Gene
IS - 2
ER -
