Rapid internalization of exogenous ganglioside GM3 and its metabolism to ceramide in human myelogenous leukemia HL-60 cells compared with control ganglioside GM1

Mitsuru Nakamura; Atsuko Tsunoda; Yusuke Furukawa; Takao Sakai; Masaki Saito

doi:10.1016/S0014-5793(96)01415-9

Rapid internalization of exogenous ganglioside GM3 and its metabolism to ceramide in human myelogenous leukemia HL-60 cells compared with control ganglioside GM1

Mitsuru Nakamura, Atsuko Tsunoda, Yusuke Furukawa, Takao Sakai, Masaki Saito

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Incorporation and metabolism of exogenous GM3 in human myelogenous leukemia HL-60 cells were analyzed using ³H-labeled GM3 ([³H]GM3). [³H]GM3 was rapidly internalized into the cells (trypsin-resistant fraction) 8 times more than the control, ³H-labeled GM1 ([³H]GM1). In addition, not only incorporation but also metabolism of [³H]GM3 was more rapid than [³H]GM1 in HL-60 cells. Moreover, one of the metabolites was found to co-migrate with ceramide in thin-layer chromatography analysis and ceramide formation from exogenous GM3 is more rapid than that from exogenous GM1. These results suggested that there would be some preferential mechanism to produce ceramide from differentiation-inducible GM3 in HL-60 cells rather than from non-inducing GM1.

Original language	English
Pages (from-to)	350-354
Number of pages	5
Journal	FEBS Letters
Volume	400
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(96)01415-9
Publication status	Published - 06-01-1997
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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@article{7363ab2cfa9a45ce9a8cf09346351b98,

title = "Rapid internalization of exogenous ganglioside GM3 and its metabolism to ceramide in human myelogenous leukemia HL-60 cells compared with control ganglioside GM1",

abstract = "Incorporation and metabolism of exogenous GM3 in human myelogenous leukemia HL-60 cells were analyzed using 3H-labeled GM3 ([3H]GM3). [3H]GM3 was rapidly internalized into the cells (trypsin-resistant fraction) 8 times more than the control, 3H-labeled GM1 ([3H]GM1). In addition, not only incorporation but also metabolism of [3H]GM3 was more rapid than [3H]GM1 in HL-60 cells. Moreover, one of the metabolites was found to co-migrate with ceramide in thin-layer chromatography analysis and ceramide formation from exogenous GM3 is more rapid than that from exogenous GM1. These results suggested that there would be some preferential mechanism to produce ceramide from differentiation-inducible GM3 in HL-60 cells rather than from non-inducing GM1.",

author = "Mitsuru Nakamura and Atsuko Tsunoda and Yusuke Furukawa and Takao Sakai and Masaki Saito",

note = "Funding Information: This work was supported in part by Grant-in-Aid for Scientific Research on Priority Areas No. 05274105 and 05274106 from the Ministry of Education, Science and Culture, Japan. We thank Dr. Handa (Tokyo Medical and Dental University) and Dr. Schwarzmann (Bonn University) for providing us with a part of tritiated GM1 ([ 3 H]GM1). We wish to thank Drs. Shinsei Gasa, Masao Iwamori for their valuable comments and support, and Ms. Yukiko Fukuda and Taeko Inageda for their technical and secretarial assistance.",

year = "1997",

month = jan,

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doi = "10.1016/S0014-5793(96)01415-9",

language = "English",

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journal = "FEBS Letters",

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T1 - Rapid internalization of exogenous ganglioside GM3 and its metabolism to ceramide in human myelogenous leukemia HL-60 cells compared with control ganglioside GM1

AU - Nakamura, Mitsuru

AU - Tsunoda, Atsuko

AU - Furukawa, Yusuke

AU - Sakai, Takao

AU - Saito, Masaki

N1 - Funding Information: This work was supported in part by Grant-in-Aid for Scientific Research on Priority Areas No. 05274105 and 05274106 from the Ministry of Education, Science and Culture, Japan. We thank Dr. Handa (Tokyo Medical and Dental University) and Dr. Schwarzmann (Bonn University) for providing us with a part of tritiated GM1 ([ 3 H]GM1). We wish to thank Drs. Shinsei Gasa, Masao Iwamori for their valuable comments and support, and Ms. Yukiko Fukuda and Taeko Inageda for their technical and secretarial assistance.

PY - 1997/1/6

Y1 - 1997/1/6

N2 - Incorporation and metabolism of exogenous GM3 in human myelogenous leukemia HL-60 cells were analyzed using 3H-labeled GM3 ([3H]GM3). [3H]GM3 was rapidly internalized into the cells (trypsin-resistant fraction) 8 times more than the control, 3H-labeled GM1 ([3H]GM1). In addition, not only incorporation but also metabolism of [3H]GM3 was more rapid than [3H]GM1 in HL-60 cells. Moreover, one of the metabolites was found to co-migrate with ceramide in thin-layer chromatography analysis and ceramide formation from exogenous GM3 is more rapid than that from exogenous GM1. These results suggested that there would be some preferential mechanism to produce ceramide from differentiation-inducible GM3 in HL-60 cells rather than from non-inducing GM1.

AB - Incorporation and metabolism of exogenous GM3 in human myelogenous leukemia HL-60 cells were analyzed using 3H-labeled GM3 ([3H]GM3). [3H]GM3 was rapidly internalized into the cells (trypsin-resistant fraction) 8 times more than the control, 3H-labeled GM1 ([3H]GM1). In addition, not only incorporation but also metabolism of [3H]GM3 was more rapid than [3H]GM1 in HL-60 cells. Moreover, one of the metabolites was found to co-migrate with ceramide in thin-layer chromatography analysis and ceramide formation from exogenous GM3 is more rapid than that from exogenous GM1. These results suggested that there would be some preferential mechanism to produce ceramide from differentiation-inducible GM3 in HL-60 cells rather than from non-inducing GM1.

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Rapid internalization of exogenous ganglioside GM3 and its metabolism to ceramide in human myelogenous leukemia HL-60 cells compared with control ganglioside GM1

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