Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green

Hiroki Ueo; Yoshiaki Shinden; Taro Tobo; Ayako Gamachi; Mitsuaki Udo; Hisateru Komatsu; Sho Nambara; Tomoko Saito; Masami Ueda; Hidenari Hirata; Shotaro Sakimura; Yuki Takano; Ryutaro Uchi; Junji Kurashige; Sayuri Akiyoshi; Tomohiro Iguchi; Hidetoshi Eguchi; Keishi Sugimachi; Yoko Kubota; Yuichiro Kai; Kenji Shibuta; Yuko Kijima; Heiji Yoshinaka; Shoji Natsugoe; Masaki Mori; Yoshihiko Maehara; Masayo Sakabe; Mako Kamiya; John W. Kakareka; Thomas J. Pohida; Peter L. Choyke; Hisataka Kobayashi; Hiroaki Ueo; Yasuteru Urano; Koshi Mimori

doi:10.1038/SREP12080

Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green

Hiroki Ueo, Yoshiaki Shinden, Taro Tobo, Ayako Gamachi, Mitsuaki Udo, Hisateru Komatsu, Sho Nambara, Tomoko Saito, Masami Ueda, Hidenari Hirata, Shotaro Sakimura, Yuki Takano, Ryutaro Uchi, Junji Kurashige, Sayuri Akiyoshi, Tomohiro Iguchi, Hidetoshi Eguchi, Keishi Sugimachi, Yoko Kubota, Yuichiro KaiKenji Shibuta, Yuko Kijima, Heiji Yoshinaka, Shoji Natsugoe, Masaki Mori, Yoshihiko Maehara, Masayo Sakabe, Mako Kamiya, John W. Kakareka, Thomas J. Pohida, Peter L. Choyke, Hisataka Kobayashi, Hiroaki Ueo, Yasuteru Urano, Koshi Mimori

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

Abstract

We previously developed γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) as a tool to detect viable cancer cells, based on the fact that the enzyme γ-glutamyltranspeptidase (GGT) is overexpressed on membranes of various cancer cells, but is not expressed in normal tissue. Cleavage of the probe by GGT generates green fluorescence. Here, we examined the feasibility of clinical application of gGlu-HMRG during breast-conserving surgery. We found that fluorescence derived from cleavage of gGlu-HMRG allowed easy discrimination of breast tumors, even those smaller than 1 mm in size, from normal mammary gland tissues, with 92% sensitivity and 94% specificity, within only 5 min after application. We believe this rapid, low-cost method represents a breakthrough in intraoperative margin assessment during breast-conserving surgery.

Original language	English
Article number	12080
Journal	Scientific reports
Volume	5
Issue number	1
DOIs	https://doi.org/10.1038/SREP12080
Publication status	Published - 2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/SREP12080

Cite this

Ueo, H., Shinden, Y., Tobo, T., Gamachi, A., Udo, M., Komatsu, H., Nambara, S., Saito, T., Ueda, M., Hirata, H., Sakimura, S., Takano, Y., Uchi, R., Kurashige, J., Akiyoshi, S., Iguchi, T., Eguchi, H., Sugimachi, K., Kubota, Y., ... Mimori, K. (2015). Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green. Scientific reports, 5(1), Article 12080. https://doi.org/10.1038/SREP12080

@article{0953907b4fe445ad918009af5476c35a,

title = "Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green",

abstract = "We previously developed γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) as a tool to detect viable cancer cells, based on the fact that the enzyme γ-glutamyltranspeptidase (GGT) is overexpressed on membranes of various cancer cells, but is not expressed in normal tissue. Cleavage of the probe by GGT generates green fluorescence. Here, we examined the feasibility of clinical application of gGlu-HMRG during breast-conserving surgery. We found that fluorescence derived from cleavage of gGlu-HMRG allowed easy discrimination of breast tumors, even those smaller than 1 mm in size, from normal mammary gland tissues, with 92% sensitivity and 94% specificity, within only 5 min after application. We believe this rapid, low-cost method represents a breakthrough in intraoperative margin assessment during breast-conserving surgery.",

author = "Hiroki Ueo and Yoshiaki Shinden and Taro Tobo and Ayako Gamachi and Mitsuaki Udo and Hisateru Komatsu and Sho Nambara and Tomoko Saito and Masami Ueda and Hidenari Hirata and Shotaro Sakimura and Yuki Takano and Ryutaro Uchi and Junji Kurashige and Sayuri Akiyoshi and Tomohiro Iguchi and Hidetoshi Eguchi and Keishi Sugimachi and Yoko Kubota and Yuichiro Kai and Kenji Shibuta and Yuko Kijima and Heiji Yoshinaka and Shoji Natsugoe and Masaki Mori and Yoshihiko Maehara and Masayo Sakabe and Mako Kamiya and Kakareka, {John W.} and Pohida, {Thomas J.} and Choyke, {Peter L.} and Hisataka Kobayashi and Hiroaki Ueo and Yasuteru Urano and Koshi Mimori",

note = "Publisher Copyright: {\textcopyright} 2015, The Author(s).",

year = "2015",

doi = "10.1038/SREP12080",

language = "English",

volume = "5",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

Ueo, H, Shinden, Y, Tobo, T, Gamachi, A, Udo, M, Komatsu, H, Nambara, S, Saito, T, Ueda, M, Hirata, H, Sakimura, S, Takano, Y, Uchi, R, Kurashige, J, Akiyoshi, S, Iguchi, T, Eguchi, H, Sugimachi, K, Kubota, Y, Kai, Y, Shibuta, K, Kijima, Y, Yoshinaka, H, Natsugoe, S, Mori, M, Maehara, Y, Sakabe, M, Kamiya, M, Kakareka, JW, Pohida, TJ, Choyke, PL, Kobayashi, H, Ueo, H, Urano, Y & Mimori, K 2015, 'Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green', Scientific reports, vol. 5, no. 1, 12080. https://doi.org/10.1038/SREP12080

TY - JOUR

T1 - Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green

AU - Ueo, Hiroki

AU - Shinden, Yoshiaki

AU - Tobo, Taro

AU - Gamachi, Ayako

AU - Udo, Mitsuaki

AU - Komatsu, Hisateru

AU - Nambara, Sho

AU - Saito, Tomoko

AU - Ueda, Masami

AU - Hirata, Hidenari

AU - Sakimura, Shotaro

AU - Takano, Yuki

AU - Uchi, Ryutaro

AU - Kurashige, Junji

AU - Akiyoshi, Sayuri

AU - Iguchi, Tomohiro

AU - Eguchi, Hidetoshi

AU - Sugimachi, Keishi

AU - Kubota, Yoko

AU - Kai, Yuichiro

AU - Shibuta, Kenji

AU - Kijima, Yuko

AU - Yoshinaka, Heiji

AU - Natsugoe, Shoji

AU - Mori, Masaki

AU - Maehara, Yoshihiko

AU - Sakabe, Masayo

AU - Kamiya, Mako

AU - Kakareka, John W.

AU - Pohida, Thomas J.

AU - Choyke, Peter L.

AU - Kobayashi, Hisataka

AU - Ueo, Hiroaki

AU - Urano, Yasuteru

AU - Mimori, Koshi

PY - 2015

Y1 - 2015

N2 - We previously developed γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) as a tool to detect viable cancer cells, based on the fact that the enzyme γ-glutamyltranspeptidase (GGT) is overexpressed on membranes of various cancer cells, but is not expressed in normal tissue. Cleavage of the probe by GGT generates green fluorescence. Here, we examined the feasibility of clinical application of gGlu-HMRG during breast-conserving surgery. We found that fluorescence derived from cleavage of gGlu-HMRG allowed easy discrimination of breast tumors, even those smaller than 1 mm in size, from normal mammary gland tissues, with 92% sensitivity and 94% specificity, within only 5 min after application. We believe this rapid, low-cost method represents a breakthrough in intraoperative margin assessment during breast-conserving surgery.

AB - We previously developed γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) as a tool to detect viable cancer cells, based on the fact that the enzyme γ-glutamyltranspeptidase (GGT) is overexpressed on membranes of various cancer cells, but is not expressed in normal tissue. Cleavage of the probe by GGT generates green fluorescence. Here, we examined the feasibility of clinical application of gGlu-HMRG during breast-conserving surgery. We found that fluorescence derived from cleavage of gGlu-HMRG allowed easy discrimination of breast tumors, even those smaller than 1 mm in size, from normal mammary gland tissues, with 92% sensitivity and 94% specificity, within only 5 min after application. We believe this rapid, low-cost method represents a breakthrough in intraoperative margin assessment during breast-conserving surgery.

UR - http://www.scopus.com/inward/record.url?scp=85018204843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018204843&partnerID=8YFLogxK

U2 - 10.1038/SREP12080

DO - 10.1038/SREP12080

M3 - Article

C2 - 26165706

AN - SCOPUS:85018204843

SN - 2045-2322

VL - 5

JO - Scientific reports

JF - Scientific reports

IS - 1

M1 - 12080

ER -

Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this