Rapid Microbial Identification and Antibiotic Resistance Detection by Mass Spectrometric Analysis of Membrane Lipids

Tao Liang, Lisa M. Leung, Belita Opene, William E. Fondrie, Young In Lee, Courtney E. Chandler, Sung Hwan Yoon, Yohei Doi, Robert K. Ernst, David R. Goodlett

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Infectious diseases have a substantial global health impact. Clinicians need rapid and accurate diagnoses of infections to direct patient treatment and improve antibiotic stewardship. Current technologies employed in routine diagnostics are based on bacterial culture followed by morphological trait differentiation and biochemical testing, which can be time-consuming and labor-intensive. With advances in mass spectrometry (MS) for clinical diagnostics, the U.S. Food and Drug Administration has approved two microbial identification platforms based on matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS analysis of microbial proteins. We recently reported a novel and complementary approach by comparing MALDI-TOF mass spectra of microbial membrane lipid fingerprints to identify ESKAPE pathogens. However, this lipid-based approach used a sample preparation method that required more than a working day from sample collection to identification. Here, we report a new method that extracts lipids efficiently and rapidly from microbial membranes using an aqueous sodium acetate (SA) buffer that can be used to identify clinically relevant Gram-positive and -negative pathogens and fungal species in less than an hour. The SA method also has the ability to differentiate antibiotic-susceptible and antibiotic-resistant strains, directly identify microbes from biological specimens, and detect multiple pathogens in a mixed sample. These results should have positive implications for the manner in which bacteria and fungi are identified in general hospital settings and intensive care units.

Original languageEnglish
Pages (from-to)1286-1294
Number of pages9
JournalAnalytical Chemistry
Volume91
Issue number2
DOIs
Publication statusPublished - 15-01-2019
Externally publishedYes

Fingerprint

Pathogens
Membrane Lipids
Sodium Acetate
Anti-Bacterial Agents
Ionization
Mass spectrometry
Desorption
Patient treatment
Lipids
Intensive care units
Lasers
Fungi
Bacteria
Buffers
Health
Personnel
Membranes
Testing
Proteins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Liang, T., Leung, L. M., Opene, B., Fondrie, W. E., Lee, Y. I., Chandler, C. E., ... Goodlett, D. R. (2019). Rapid Microbial Identification and Antibiotic Resistance Detection by Mass Spectrometric Analysis of Membrane Lipids. Analytical Chemistry, 91(2), 1286-1294. https://doi.org/10.1021/acs.analchem.8b02611
Liang, Tao ; Leung, Lisa M. ; Opene, Belita ; Fondrie, William E. ; Lee, Young In ; Chandler, Courtney E. ; Yoon, Sung Hwan ; Doi, Yohei ; Ernst, Robert K. ; Goodlett, David R. / Rapid Microbial Identification and Antibiotic Resistance Detection by Mass Spectrometric Analysis of Membrane Lipids. In: Analytical Chemistry. 2019 ; Vol. 91, No. 2. pp. 1286-1294.
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Liang, T, Leung, LM, Opene, B, Fondrie, WE, Lee, YI, Chandler, CE, Yoon, SH, Doi, Y, Ernst, RK & Goodlett, DR 2019, 'Rapid Microbial Identification and Antibiotic Resistance Detection by Mass Spectrometric Analysis of Membrane Lipids', Analytical Chemistry, vol. 91, no. 2, pp. 1286-1294. https://doi.org/10.1021/acs.analchem.8b02611

Rapid Microbial Identification and Antibiotic Resistance Detection by Mass Spectrometric Analysis of Membrane Lipids. / Liang, Tao; Leung, Lisa M.; Opene, Belita; Fondrie, William E.; Lee, Young In; Chandler, Courtney E.; Yoon, Sung Hwan; Doi, Yohei; Ernst, Robert K.; Goodlett, David R.

In: Analytical Chemistry, Vol. 91, No. 2, 15.01.2019, p. 1286-1294.

Research output: Contribution to journalArticle

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AU - Goodlett, David R.

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