Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR

Kazuoki Osumi, Takafumi Fukui, Hitoshi Kiyoi, Masanobu Kasai, Yoshihisa Kodera, Kazuko Kudo, Koji Kato, Takaharu Matsuyama, Kazuyuki Naito, Mitsune Tanimoto, Hisamaru Hirai, Hidehiko Saito, Ryuzo Ohno, Tomoki Naoe

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We established a real-time PCR method that can simultaneously detect 10 different fusion transcripts (major, minor and micro BCR/ABL, AML1/MTG8, PML/RARα, CBFβ/MYH11, TEL/AML1, E2A/PBX1, MLL/AF4, and MLL/AF9) together with Wilms' tumor gene (WT1) transcripts. This screening method allowed the processing of six specimens concomitantly and required only one working day from RNA extraction to final results. Fifty-seven bone marrow (BM) samples from patients with acute leukemia were retrospectively screened for the presence of fusion and WT1 transcripts without knowledge of the cytogenetic data, and the fusion transcripts were detected in 20 of 57 samples (35.1%). The concordance between the present method and cytogenetic analysis was examined in 38 samples in which the cytogenetic data were available. In 12 of 38 samples, the PCR results agreed with the cytogenetic data, whereas in 4 of the remaining 26 samples, the translocations were detected by real-time PCR alone because of the insufficient number of metaphases obtained and presumably the submicroscopic or masked translocations. The WT1 levels ranged from 400 to 690,000 copies/μg RNA in BM from leukemia patients, whereas 0-470 copies/μg RNA were found in BM cells from BMT donors. This real-time PCR method enables rapid and efficient characterization of acute leukemia in addition to subsequent evaluation of minimal residual diseases.

Original languageEnglish
Pages (from-to)2291-2299
Number of pages9
JournalLeukemia and Lymphoma
Volume43
Issue number12
DOIs
Publication statusPublished - 01-12-2002

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR'. Together they form a unique fingerprint.

  • Cite this

    Osumi, K., Fukui, T., Kiyoi, H., Kasai, M., Kodera, Y., Kudo, K., Kato, K., Matsuyama, T., Naito, K., Tanimoto, M., Hirai, H., Saito, H., Ohno, R., & Naoe, T. (2002). Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR. Leukemia and Lymphoma, 43(12), 2291-2299. https://doi.org/10.1080/1042819021000040206