Rapid, simple and simultaneous measurement of kynurenine and tryptophan in plasma by column switching-HPLC method

K. Kawai, H. Ishikawa, K. Ohashi, Y. Itoh, R. Teradaira

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We established a highly reproducible, simple and rapid simultaneous measurement method in which blood plasma can be directly injected into high-performance liquid chromatography (HPLC) without conducting conventional extraction of kynurenine (Kyn) and tryptophan (Trp) from blood plasma. In this method, Kyn and Trp were first separated from proteins present in blood plasma using a pre-column. Then, by column-switching, the separated Kyn and Trp were automatically injected into a separation column. The recoveries of Kyn and Trp determined in blood plasma by this method were 100.0 ± 0.9 and 100.0 ± 1.4%, respectively.

Original languageEnglish
Pages (from-to)415-419
Number of pages5
JournalInternational Congress Series
Volume1304
DOIs
Publication statusPublished - 01-11-2007

Fingerprint

Kynurenine
Tryptophan
High Pressure Liquid Chromatography
Proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{44ea125107a64af28a3c5f7c1434bb42,
title = "Rapid, simple and simultaneous measurement of kynurenine and tryptophan in plasma by column switching-HPLC method",
abstract = "We established a highly reproducible, simple and rapid simultaneous measurement method in which blood plasma can be directly injected into high-performance liquid chromatography (HPLC) without conducting conventional extraction of kynurenine (Kyn) and tryptophan (Trp) from blood plasma. In this method, Kyn and Trp were first separated from proteins present in blood plasma using a pre-column. Then, by column-switching, the separated Kyn and Trp were automatically injected into a separation column. The recoveries of Kyn and Trp determined in blood plasma by this method were 100.0 ± 0.9 and 100.0 ± 1.4{\%}, respectively.",
author = "K. Kawai and H. Ishikawa and K. Ohashi and Y. Itoh and R. Teradaira",
year = "2007",
month = "11",
day = "1",
doi = "10.1016/j.ics.2007.07.005",
language = "English",
volume = "1304",
pages = "415--419",
journal = "International Congress Series",
issn = "0531-5131",
publisher = "Elsevier BV",

}

Rapid, simple and simultaneous measurement of kynurenine and tryptophan in plasma by column switching-HPLC method. / Kawai, K.; Ishikawa, H.; Ohashi, K.; Itoh, Y.; Teradaira, R.

In: International Congress Series, Vol. 1304, 01.11.2007, p. 415-419.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rapid, simple and simultaneous measurement of kynurenine and tryptophan in plasma by column switching-HPLC method

AU - Kawai, K.

AU - Ishikawa, H.

AU - Ohashi, K.

AU - Itoh, Y.

AU - Teradaira, R.

PY - 2007/11/1

Y1 - 2007/11/1

N2 - We established a highly reproducible, simple and rapid simultaneous measurement method in which blood plasma can be directly injected into high-performance liquid chromatography (HPLC) without conducting conventional extraction of kynurenine (Kyn) and tryptophan (Trp) from blood plasma. In this method, Kyn and Trp were first separated from proteins present in blood plasma using a pre-column. Then, by column-switching, the separated Kyn and Trp were automatically injected into a separation column. The recoveries of Kyn and Trp determined in blood plasma by this method were 100.0 ± 0.9 and 100.0 ± 1.4%, respectively.

AB - We established a highly reproducible, simple and rapid simultaneous measurement method in which blood plasma can be directly injected into high-performance liquid chromatography (HPLC) without conducting conventional extraction of kynurenine (Kyn) and tryptophan (Trp) from blood plasma. In this method, Kyn and Trp were first separated from proteins present in blood plasma using a pre-column. Then, by column-switching, the separated Kyn and Trp were automatically injected into a separation column. The recoveries of Kyn and Trp determined in blood plasma by this method were 100.0 ± 0.9 and 100.0 ± 1.4%, respectively.

UR - http://www.scopus.com/inward/record.url?scp=35948946832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35948946832&partnerID=8YFLogxK

U2 - 10.1016/j.ics.2007.07.005

DO - 10.1016/j.ics.2007.07.005

M3 - Article

AN - SCOPUS:35948946832

VL - 1304

SP - 415

EP - 419

JO - International Congress Series

JF - International Congress Series

SN - 0531-5131

ER -