TY - JOUR
T1 - Rapid, simple and simultaneous measurement of kynurenine and tryptophan in plasma by column switching-HPLC method
AU - Kawai, K.
AU - Ishikawa, H.
AU - Ohashi, K.
AU - Itoh, Y.
AU - Teradaira, R.
PY - 2007/11/1
Y1 - 2007/11/1
N2 - We established a highly reproducible, simple and rapid simultaneous measurement method in which blood plasma can be directly injected into high-performance liquid chromatography (HPLC) without conducting conventional extraction of kynurenine (Kyn) and tryptophan (Trp) from blood plasma. In this method, Kyn and Trp were first separated from proteins present in blood plasma using a pre-column. Then, by column-switching, the separated Kyn and Trp were automatically injected into a separation column. The recoveries of Kyn and Trp determined in blood plasma by this method were 100.0 ± 0.9 and 100.0 ± 1.4%, respectively.
AB - We established a highly reproducible, simple and rapid simultaneous measurement method in which blood plasma can be directly injected into high-performance liquid chromatography (HPLC) without conducting conventional extraction of kynurenine (Kyn) and tryptophan (Trp) from blood plasma. In this method, Kyn and Trp were first separated from proteins present in blood plasma using a pre-column. Then, by column-switching, the separated Kyn and Trp were automatically injected into a separation column. The recoveries of Kyn and Trp determined in blood plasma by this method were 100.0 ± 0.9 and 100.0 ± 1.4%, respectively.
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U2 - 10.1016/j.ics.2007.07.005
DO - 10.1016/j.ics.2007.07.005
M3 - Article
AN - SCOPUS:35948946832
SN - 0531-5131
VL - 1304
SP - 415
EP - 419
JO - International Congress Series
JF - International Congress Series
ER -