Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices

Miaomiao Sun, Toshihiro Kasama, Noritada Kaji, Shin Ichi Akiyama, Yukio Yuzawa, Seiichi Matsuo, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We have developed immuno-pillar devices for rapid and easy-to-use immunoassay, but to improve the speed of assay (5-10 min) and the detection sensitivity (nM-pM level) has still remained as major problems toward the clinical applications of immuno-pillar devices. We report here the second-generation immuno-pillar devices with faster assay within 2 min and pM-fM detection sensitivity. New devices enable us to apply them to the clinical trials for the detection of multiple biomarkers (monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), liver-type fatty acid binding protein (L-FABP)) of diabetic nephropathy in human urine without any pretreatments.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages812-814
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 01-01-2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28-10-201201-11-2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period28-10-1201-11-12

Fingerprint

Assays
Fatty Acid-Binding Proteins
Angiotensinogen
Chemokine CCL2
Biomarkers
Fatty acids
Liver
Proteins
Carrier Proteins

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Sun, M., Kasama, T., Kaji, N., Akiyama, S. I., Yuzawa, Y., Matsuo, S., ... Baba, Y. (2012). Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 812-814). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.
Sun, Miaomiao ; Kasama, Toshihiro ; Kaji, Noritada ; Akiyama, Shin Ichi ; Yuzawa, Yukio ; Matsuo, Seiichi ; Tokeshi, Manabu ; Baba, Yoshinobu. / Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 812-814 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
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author = "Miaomiao Sun and Toshihiro Kasama and Noritada Kaji and Akiyama, {Shin Ichi} and Yukio Yuzawa and Seiichi Matsuo and Manabu Tokeshi and Yoshinobu Baba",
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Sun, M, Kasama, T, Kaji, N, Akiyama, SI, Yuzawa, Y, Matsuo, S, Tokeshi, M & Baba, Y 2012, Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 812-814, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 28-10-12.

Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. / Sun, Miaomiao; Kasama, Toshihiro; Kaji, Noritada; Akiyama, Shin Ichi; Yuzawa, Yukio; Matsuo, Seiichi; Tokeshi, Manabu; Baba, Yoshinobu.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 812-814 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - We have developed immuno-pillar devices for rapid and easy-to-use immunoassay, but to improve the speed of assay (5-10 min) and the detection sensitivity (nM-pM level) has still remained as major problems toward the clinical applications of immuno-pillar devices. We report here the second-generation immuno-pillar devices with faster assay within 2 min and pM-fM detection sensitivity. New devices enable us to apply them to the clinical trials for the detection of multiple biomarkers (monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), liver-type fatty acid binding protein (L-FABP)) of diabetic nephropathy in human urine without any pretreatments.

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Sun M, Kasama T, Kaji N, Akiyama SI, Yuzawa Y, Matsuo S et al. Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 812-814. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).