Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices

Miaomiao Sun; Toshihiro Kasama; Noritada Kaji; Shin Ichi Akiyama; Yukio Yuzawa; Seiichi Matsuo; Manabu Tokeshi; Yoshinobu Baba

Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices

Miaomiao Sun
, Toshihiro Kasama
, Noritada Kaji
, Shin Ichi Akiyama
, Yukio Yuzawa
, Seiichi Matsuo
, Manabu Tokeshi
, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have developed immuno-pillar devices for rapid and easy-to-use immunoassay, but to improve the speed of assay (5-10 min) and the detection sensitivity (nM-pM level) has still remained as major problems toward the clinical applications of immuno-pillar devices. We report here the second-generation immuno-pillar devices with faster assay within 2 min and pM-fM detection sensitivity. New devices enable us to apply them to the clinical trials for the detection of multiple biomarkers (monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), liver-type fatty acid binding protein (L-FABP)) of diabetic nephropathy in human urine without any pretreatments.

Original language	English
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publisher	Chemical and Biological Microsystems Society
Pages	812-814
Number of pages	3
ISBN (Print)	9780979806452
Publication status	Published - 2012
Externally published	Yes
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 28-10-2012 → 01-11-2012

Publication series

Name	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/Territory	Japan
City	Okinawa
Period	28-10-12 → 01-11-12

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Sun, M., Kasama, T., Kaji, N., Akiyama, S. I., Yuzawa, Y., Matsuo, S., Tokeshi, M., & Baba, Y. (2012). Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 812-814). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.

Sun, Miaomiao ; Kasama, Toshihiro ; Kaji, Noritada et al. / Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 812-814 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

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title = "Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices",

abstract = "We have developed immuno-pillar devices for rapid and easy-to-use immunoassay, but to improve the speed of assay (5-10 min) and the detection sensitivity (nM-pM level) has still remained as major problems toward the clinical applications of immuno-pillar devices. We report here the second-generation immuno-pillar devices with faster assay within 2 min and pM-fM detection sensitivity. New devices enable us to apply them to the clinical trials for the detection of multiple biomarkers (monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), liver-type fatty acid binding protein (L-FABP)) of diabetic nephropathy in human urine without any pretreatments.",

author = "Miaomiao Sun and Toshihiro Kasama and Noritada Kaji and Akiyama, \{Shin Ichi\} and Yukio Yuzawa and Seiichi Matsuo and Manabu Tokeshi and Yoshinobu Baba",

year = "2012",

language = "English",

isbn = "9780979806452",

series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",

publisher = "Chemical and Biological Microsystems Society",

pages = "812--814",

booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",

note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",

}

Sun, M, Kasama, T, Kaji, N, Akiyama, SI, Yuzawa, Y, Matsuo, S, Tokeshi, M & Baba, Y 2012, Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 812-814, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 28-10-12.

Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. / Sun, Miaomiao; Kasama, Toshihiro; Kaji, Noritada et al.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 812-814 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices

AU - Sun, Miaomiao

AU - Kasama, Toshihiro

AU - Kaji, Noritada

AU - Akiyama, Shin Ichi

AU - Yuzawa, Yukio

AU - Matsuo, Seiichi

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

PY - 2012

Y1 - 2012

N2 - We have developed immuno-pillar devices for rapid and easy-to-use immunoassay, but to improve the speed of assay (5-10 min) and the detection sensitivity (nM-pM level) has still remained as major problems toward the clinical applications of immuno-pillar devices. We report here the second-generation immuno-pillar devices with faster assay within 2 min and pM-fM detection sensitivity. New devices enable us to apply them to the clinical trials for the detection of multiple biomarkers (monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), liver-type fatty acid binding protein (L-FABP)) of diabetic nephropathy in human urine without any pretreatments.

AB - We have developed immuno-pillar devices for rapid and easy-to-use immunoassay, but to improve the speed of assay (5-10 min) and the detection sensitivity (nM-pM level) has still remained as major problems toward the clinical applications of immuno-pillar devices. We report here the second-generation immuno-pillar devices with faster assay within 2 min and pM-fM detection sensitivity. New devices enable us to apply them to the clinical trials for the detection of multiple biomarkers (monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), liver-type fatty acid binding protein (L-FABP)) of diabetic nephropathy in human urine without any pretreatments.

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M3 - Conference contribution

AN - SCOPUS:84901796095

SN - 9780979806452

T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

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PB - Chemical and Biological Microsystems Society

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Y2 - 28 October 2012 through 1 November 2012

ER -

Sun M, Kasama T, Kaji N, Akiyama SI, Yuzawa Y, Matsuo S et al. Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 812-814. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Rapid urine-based clinical diagnosis of diabetic nephropathy with femto-molar sensitivity by immuno-pillar devices

Abstract

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All Science Journal Classification (ASJC) codes

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