Rat T lymphocyte antigens comparable with mouse Lyt-1 and Lyt-2,3 antigenic systems

Characterization by monoclonal antibodies

Akihiro Matsuura, Y. Ishii, H. Yuasa, H. Narita, S. Kon, T. Takami, K. Kikuchi

Research output: Contribution to journalArticle

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Abstract

Rat T lymphocyte antigens were defined by using two distinct monoclonal antibodies, R1-3B3 and R1-10B5). R1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, stained almost all of thymus and T cells but not the majority of B cells and bone marrow cells. The antigen defined by R1-3B3 existed more abundantly on medullary thymocytes and peripheral T cells than on cortical thymocytes. Immunochemical data showed that R1-3B3 antibody recognized a single glycoprotein with a m.w. of 67,000, showing marked electric charge heterogeneity with isoelectric points ranging from 5.4 to 7.3. R1-10B5 antibody, on the other hand, had more restricted reactivity with rat T cells hand labeled approximately 85% of thymus cells and 30% of the peripheral T cells but neither B cells nor bone marrow cells. These T cells positive for R1-10B5 appeared to be negative for W3/25 antigen, which has been shown to be the marker for the rat T cell subset associated with helper function. R1-10B5 antibody detected a basic glycoprotein complex consisting of sulfhydryl-linked subunits with 30,000 and 34.000 m.w. Although the antigen defined by R1-3B3 was resistant to trypsin digestion, the one detected by R1-10B5 was much more sensitive to trypsin cleavage. All of these data obtained with either R1-3B3 or R1-10B5 are quite comparable to those reported for mouse Lyt-1 or Lyt-2,3 antigens, and thus suggest that the antigens defined by R1-3B3 and R1-10B5 antibodies represent rat homologues of Lyt-1 and Lyt-2,3 antigens in the murine system, respectively.

Original languageEnglish
Pages (from-to)316-322
Number of pages7
JournalJournal of Immunology
Volume132
Issue number1
Publication statusPublished - 23-02-1984

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Viral Tumor Antigens
Monoclonal Antibodies
T-Lymphocytes
Antigens
Antibodies
Thymocytes
Bone Marrow Cells
Trypsin
Thymus Gland
Glycoproteins
B-Lymphocytes
Isoelectric Point
T-Lymphocyte Subsets
Fluorescent Antibody Technique
Digestion
Hand
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Matsuura, Akihiro ; Ishii, Y. ; Yuasa, H. ; Narita, H. ; Kon, S. ; Takami, T. ; Kikuchi, K. / Rat T lymphocyte antigens comparable with mouse Lyt-1 and Lyt-2,3 antigenic systems : Characterization by monoclonal antibodies. In: Journal of Immunology. 1984 ; Vol. 132, No. 1. pp. 316-322.
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abstract = "Rat T lymphocyte antigens were defined by using two distinct monoclonal antibodies, R1-3B3 and R1-10B5). R1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, stained almost all of thymus and T cells but not the majority of B cells and bone marrow cells. The antigen defined by R1-3B3 existed more abundantly on medullary thymocytes and peripheral T cells than on cortical thymocytes. Immunochemical data showed that R1-3B3 antibody recognized a single glycoprotein with a m.w. of 67,000, showing marked electric charge heterogeneity with isoelectric points ranging from 5.4 to 7.3. R1-10B5 antibody, on the other hand, had more restricted reactivity with rat T cells hand labeled approximately 85{\%} of thymus cells and 30{\%} of the peripheral T cells but neither B cells nor bone marrow cells. These T cells positive for R1-10B5 appeared to be negative for W3/25 antigen, which has been shown to be the marker for the rat T cell subset associated with helper function. R1-10B5 antibody detected a basic glycoprotein complex consisting of sulfhydryl-linked subunits with 30,000 and 34.000 m.w. Although the antigen defined by R1-3B3 was resistant to trypsin digestion, the one detected by R1-10B5 was much more sensitive to trypsin cleavage. All of these data obtained with either R1-3B3 or R1-10B5 are quite comparable to those reported for mouse Lyt-1 or Lyt-2,3 antigens, and thus suggest that the antigens defined by R1-3B3 and R1-10B5 antibodies represent rat homologues of Lyt-1 and Lyt-2,3 antigens in the murine system, respectively.",
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Rat T lymphocyte antigens comparable with mouse Lyt-1 and Lyt-2,3 antigenic systems : Characterization by monoclonal antibodies. / Matsuura, Akihiro; Ishii, Y.; Yuasa, H.; Narita, H.; Kon, S.; Takami, T.; Kikuchi, K.

In: Journal of Immunology, Vol. 132, No. 1, 23.02.1984, p. 316-322.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rat T lymphocyte antigens comparable with mouse Lyt-1 and Lyt-2,3 antigenic systems

T2 - Characterization by monoclonal antibodies

AU - Matsuura, Akihiro

AU - Ishii, Y.

AU - Yuasa, H.

AU - Narita, H.

AU - Kon, S.

AU - Takami, T.

AU - Kikuchi, K.

PY - 1984/2/23

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N2 - Rat T lymphocyte antigens were defined by using two distinct monoclonal antibodies, R1-3B3 and R1-10B5). R1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, stained almost all of thymus and T cells but not the majority of B cells and bone marrow cells. The antigen defined by R1-3B3 existed more abundantly on medullary thymocytes and peripheral T cells than on cortical thymocytes. Immunochemical data showed that R1-3B3 antibody recognized a single glycoprotein with a m.w. of 67,000, showing marked electric charge heterogeneity with isoelectric points ranging from 5.4 to 7.3. R1-10B5 antibody, on the other hand, had more restricted reactivity with rat T cells hand labeled approximately 85% of thymus cells and 30% of the peripheral T cells but neither B cells nor bone marrow cells. These T cells positive for R1-10B5 appeared to be negative for W3/25 antigen, which has been shown to be the marker for the rat T cell subset associated with helper function. R1-10B5 antibody detected a basic glycoprotein complex consisting of sulfhydryl-linked subunits with 30,000 and 34.000 m.w. Although the antigen defined by R1-3B3 was resistant to trypsin digestion, the one detected by R1-10B5 was much more sensitive to trypsin cleavage. All of these data obtained with either R1-3B3 or R1-10B5 are quite comparable to those reported for mouse Lyt-1 or Lyt-2,3 antigens, and thus suggest that the antigens defined by R1-3B3 and R1-10B5 antibodies represent rat homologues of Lyt-1 and Lyt-2,3 antigens in the murine system, respectively.

AB - Rat T lymphocyte antigens were defined by using two distinct monoclonal antibodies, R1-3B3 and R1-10B5). R1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, stained almost all of thymus and T cells but not the majority of B cells and bone marrow cells. The antigen defined by R1-3B3 existed more abundantly on medullary thymocytes and peripheral T cells than on cortical thymocytes. Immunochemical data showed that R1-3B3 antibody recognized a single glycoprotein with a m.w. of 67,000, showing marked electric charge heterogeneity with isoelectric points ranging from 5.4 to 7.3. R1-10B5 antibody, on the other hand, had more restricted reactivity with rat T cells hand labeled approximately 85% of thymus cells and 30% of the peripheral T cells but neither B cells nor bone marrow cells. These T cells positive for R1-10B5 appeared to be negative for W3/25 antigen, which has been shown to be the marker for the rat T cell subset associated with helper function. R1-10B5 antibody detected a basic glycoprotein complex consisting of sulfhydryl-linked subunits with 30,000 and 34.000 m.w. Although the antigen defined by R1-3B3 was resistant to trypsin digestion, the one detected by R1-10B5 was much more sensitive to trypsin cleavage. All of these data obtained with either R1-3B3 or R1-10B5 are quite comparable to those reported for mouse Lyt-1 or Lyt-2,3 antigens, and thus suggest that the antigens defined by R1-3B3 and R1-10B5 antibodies represent rat homologues of Lyt-1 and Lyt-2,3 antigens in the murine system, respectively.

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