Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

Takeshi Onoue, Motomitsu Goto, Takashi Tominaga, Mariko Sugiyama, Taku Tsunekawa, Daisuke Hagiwara, Ryoichi Banno, Hidetaka Suga, Yoshihisa Sugimura, Hiroshi Arima

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Abstract

In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H2O2 increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalNeuroscience Letters
Volume619
DOIs
Publication statusPublished - 21-04-2016

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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    Onoue, T., Goto, M., Tominaga, T., Sugiyama, M., Tsunekawa, T., Hagiwara, D., Banno, R., Suga, H., Sugimura, Y., & Arima, H. (2016). Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus. Neuroscience Letters, 619, 1-7. https://doi.org/10.1016/j.neulet.2016.03.011