Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

Takeshi Onoue; Motomitsu Goto; Takashi Tominaga; Mariko Sugiyama; Taku Tsunekawa; Daisuke Hagiwara; Ryoichi Banno; Hidetaka Suga; Yoshihisa Sugimura; Hiroshi Arima

doi:10.1016/j.neulet.2016.03.011

Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

Takeshi Onoue, Motomitsu Goto, Takashi Tominaga, Mariko Sugiyama, Taku Tsunekawa, Daisuke Hagiwara, Ryoichi Banno, Hidetaka Suga, Yoshihisa Sugimura, Hiroshi Arima

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H₂O₂ increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Neuroscience Letters
Volume	619
DOIs	https://doi.org/10.1016/j.neulet.2016.03.011
Publication status	Published - 21-04-2016
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1016/j.neulet.2016.03.011

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title = "Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus",

abstract = "In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H2O2 increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.",

author = "Takeshi Onoue and Motomitsu Goto and Takashi Tominaga and Mariko Sugiyama and Taku Tsunekawa and Daisuke Hagiwara and Ryoichi Banno and Hidetaka Suga and Yoshihisa Sugimura and Hiroshi Arima",

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T1 - Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

AU - Onoue, Takeshi

AU - Goto, Motomitsu

AU - Tominaga, Takashi

AU - Sugiyama, Mariko

AU - Tsunekawa, Taku

AU - Hagiwara, Daisuke

AU - Banno, Ryoichi

AU - Suga, Hidetaka

AU - Sugimura, Yoshihisa

AU - Arima, Hiroshi

PY - 2016/4/21

Y1 - 2016/4/21

N2 - In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H2O2 increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.

AB - In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H2O2 increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.

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JO - Neuroscience Letters

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ER -

Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

Abstract

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