Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus

Takeshi Onoue, Motomitsu Goto, Takashi Tominaga, Mariko Sugiyama, Taku Tsunekawa, Daisuke Hagiwara, Ryoichi Banno, Hidetaka Suga, Yoshihisa Sugimura, Hiroshi Arima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In the hypothalamus, several reports have implied that ROS mediate physiological effects of insulin. In this study, we investigated the mechanisms of insulin-induced ROS production and the effect of ROS on insulin signal transduction in mouse hypothalamic organotypic cultures. Insulin increased intracellular ROS, which were suppressed by NADPH oxidase inhibitor. H2O2 increased phospho-insulin receptor β (p-IRβ) and phospho-Akt (p-Akt) levels. Insulin-induced increases in p-IRβ and p-Akt levels were attenuated by ROS scavenger or NADPH oxidase inhibitor. Our data suggest that insulin-induced phosphorylation of IRβ and Akt is mediated via ROS which are predominantly produced by NADPH oxidase in mouse hypothalamus.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalNeuroscience Letters
Volume619
DOIs
Publication statusPublished - 21-04-2016

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Hypothalamus
Reactive Oxygen Species
Signal Transduction
Insulin
NADPH Oxidase
Insulin Receptor
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Onoue, T., Goto, M., Tominaga, T., Sugiyama, M., Tsunekawa, T., Hagiwara, D., ... Arima, H. (2016). Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus. Neuroscience Letters, 619, 1-7. https://doi.org/10.1016/j.neulet.2016.03.011
Onoue, Takeshi ; Goto, Motomitsu ; Tominaga, Takashi ; Sugiyama, Mariko ; Tsunekawa, Taku ; Hagiwara, Daisuke ; Banno, Ryoichi ; Suga, Hidetaka ; Sugimura, Yoshihisa ; Arima, Hiroshi. / Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus. In: Neuroscience Letters. 2016 ; Vol. 619. pp. 1-7.
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Onoue, T, Goto, M, Tominaga, T, Sugiyama, M, Tsunekawa, T, Hagiwara, D, Banno, R, Suga, H, Sugimura, Y & Arima, H 2016, 'Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus', Neuroscience Letters, vol. 619, pp. 1-7. https://doi.org/10.1016/j.neulet.2016.03.011

Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus. / Onoue, Takeshi; Goto, Motomitsu; Tominaga, Takashi; Sugiyama, Mariko; Tsunekawa, Taku; Hagiwara, Daisuke; Banno, Ryoichi; Suga, Hidetaka; Sugimura, Yoshihisa; Arima, Hiroshi.

In: Neuroscience Letters, Vol. 619, 21.04.2016, p. 1-7.

Research output: Contribution to journalArticle

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AU - Onoue, Takeshi

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AU - Hagiwara, Daisuke

AU - Banno, Ryoichi

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AU - Arima, Hiroshi

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Onoue T, Goto M, Tominaga T, Sugiyama M, Tsunekawa T, Hagiwara D et al. Reactive oxygen species mediate insulin signal transduction in mouse hypothalamus. Neuroscience Letters. 2016 Apr 21;619:1-7. https://doi.org/10.1016/j.neulet.2016.03.011