Rearrangement of immunoglobulin light chain and heavy chain constant region genes in multiple myeloma

F. Maruyama, T. Ino, H. Miyazaki, T. Nomura, K. Shinkai, M. Wakita, H. Kojima, R. Sobue, M. Okamoto, T. Matui

Research output: Contribution to journalArticlepeer-review

Abstract

We analysed immunoglobulin (Ig) gene rearrangements in 28 patients with multiple myeloma by Southern hybridization method. We used 5 probes which cover C kappa and kappa de loci of Ig light chain kappa gene, and JH, 5'S mu and S gamma 3 loci of Ig heavy chain gene. In 11 out of 12 patients with kappa-producing myeloma, DNA rearrangements were observed using C kappa probe. Among them, kappa de region was rearranged in 7 patients and kept germline configuration in 4 patients. In all of 14 patients with lambda-producing myeloma, C kappa region was deleted and kappa de region was rearranged. 5'S mu-probe was very useful for detecting class switch recombination, and furthermore by using S gamma-probe together, S mu-S gamma joining could be detected. In all of 10 patients with gamma-producing myeloma, 5'S mu and S gamma-probes detected the rearranged band of the same size on at least 1 allele, which suggested the presence of S mu-S gamma joinings. In 8 of 10 patients with Bence-Jones myeloma, 5'S mu-probe detected rearranged bands and the presence of class switch recombinations were suggested as observed in other Ig secretory myelomas. In other 2 patients with Bence-Jones myeloma, non-functional class switch recombinations were detected. The results of this study indicated that genotypes corresponded well to phenotypes in multiple myeloma, and further analysis in other types of B cell malignancies will be interesting.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
Journal[Rinshō ketsueki] The Japanese journal of clinical hematology
Volume33
Issue number1
Publication statusPublished - 01-1992

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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