Receptor activator of nuclear factor-kappa B ligand induces osteoclast formation in RAW 264.7 macrophage cells via augmented production of macrophage-colony-stimulating factor

Shamima Islam, Ferdaus Hassan, Gantsetseg Tumurkhuu, Jargalsaikhan Dagvadorj, Naoki Koide, Yoshikazu Naiki, Tomoaki Yoshida, Takashi Yokochi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

RAW 264.7 macrophage cells differentiate into osteoclast-like cells in the presence of RANKL. Participation of M-CSF in RANKL-induced osteoclast formation of RAW 264.7 cells was examined. TRAP-positive osteoclast-like cells appeared in RAW 264.7 cells cultured in the presence of RANKL. RANKL-induced osteoclast formation was markedly inhibited by anti-M-CSF antibody.RANKL augmented M-CSFmRNA expression and M-CSF production in RAW 264.7 cells. Further, anti-M-CSF antibody inhibited the expression of RANK, c-fms, c-fos and TRAP mRNA in RANKL-stimulated RAW 264.7 cells. However, anti-M-CSF antibody did not affect the expression of DC-STAMP in the stimulated cells. Therefore, RANKL was suggested to induce osteoclast formation in RAW 264.7 cells via augmented production of M-CSF. The putative role of M-CSF in RANKL-induced osteoclast formation of RAW 264.7 cells is discussed.

Original languageEnglish
Pages (from-to)585-590
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume52
Issue number12
DOIs
Publication statusPublished - 12-2008

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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