TY - JOUR
T1 - Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro
AU - Shimomura, Yasuyo
AU - Suga, Mika
AU - Kuriyama, Naohide
AU - Nakamura, Tomoyuki
AU - Sakai, Toshikazu
AU - Kato, Yu
AU - Hara, Yoshitaka
AU - Yamashita, Chizuru
AU - Nagasaki, Hiroshi
AU - Kaneki, Masao
AU - Nishida, Osamu
N1 - Publisher Copyright:
© 2016 The Author(s).
PY - 2016/7/22
Y1 - 2016/7/22
N2 - The aim of this study was to investigate the effects of recombinant human-soluble thrombomodulin (rTM) on lipopolysaccharide (LPS)-induced, platelet-dependent neutrophil extracellular trap (NET) formation (NETosis). Human peripheral blood neutrophils and platelets were co-incubated with or without LPS (0.2 μg/ml) in the presence and absence of rTM (2 μg/ml). NETosis was confirmed by immunostaining and confocal microscopy. In the absence of platelets, LPS did not induce NETosis in the neutrophils. NETosis, however, was induced by LPS when neutrophils were co-cultured with platelets (64 % of neutrophils). Notably, rTM was able to fully inhibit NETosis in neutrophils cultured with platelets and in the presence of LPS. rTM did not induce NETosis in this co-culture system (p < 0.01 versus LPS in the absence of rTM). These results show that rTM can suppress LPS-induced platelet-dependent NETosis in vitro.
AB - The aim of this study was to investigate the effects of recombinant human-soluble thrombomodulin (rTM) on lipopolysaccharide (LPS)-induced, platelet-dependent neutrophil extracellular trap (NET) formation (NETosis). Human peripheral blood neutrophils and platelets were co-incubated with or without LPS (0.2 μg/ml) in the presence and absence of rTM (2 μg/ml). NETosis was confirmed by immunostaining and confocal microscopy. In the absence of platelets, LPS did not induce NETosis in the neutrophils. NETosis, however, was induced by LPS when neutrophils were co-cultured with platelets (64 % of neutrophils). Notably, rTM was able to fully inhibit NETosis in neutrophils cultured with platelets and in the presence of LPS. rTM did not induce NETosis in this co-culture system (p < 0.01 versus LPS in the absence of rTM). These results show that rTM can suppress LPS-induced platelet-dependent NETosis in vitro.
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U2 - 10.1186/s40560-016-0177-9
DO - 10.1186/s40560-016-0177-9
M3 - Letter
AN - SCOPUS:84997294275
SN - 2052-0492
VL - 4
JO - Journal of Intensive Care
JF - Journal of Intensive Care
IS - 1
M1 - 48
ER -