Reduced ischemic brain injury by partial rejuvenation of bone marrow cells in aged rats

Akihiko Taguchi, Pengxiang Zhu, Fang Cao, Akie Kikuchi-Taura, Yukiko Kasahara, David M. Stern, Toshihiro Soma, Tomohiro Matsuyama, Ryuji Hata

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Circulating bone marrow-derived immature cells, including endothelial progenitor cells, have been implicated in homeostasis of the microvasculature. Decreased levels of circulating endothelial progenitor cells, associated with aging and/or cardiovascular risk factors, correlate with poor clinical outcomes in a range of cardiovascular diseases. Herein, we transplanted bone marrow cells from young stroke-prone spontaneously hypertensive rats (SHR-SP) into aged SHR-SP, the latter not exposed to radiation or chemotherapy. Analysis of recipient peripheral blood 28 days after transplantation revealed that 5% of circulating blood cells were of donor origin. Cerebral infarction was induced on day 30 posttransplantation. Animals transplanted with bone marrow from young SHR-SP displayed an increase in density of the microvasculature in the periinfarction zone, reduced ischemic brain damage and improved neurologic function. In vitro analysis revealed enhanced activation of endothelial nitric oxide synthase and reduced activation p38 microtubule-associated protein (MAP) kinase, the latter associated with endothelial apoptosis, in cultures exposed to bone marrow-derived mononuclear cells from young animals versus cells from aged counterparts. Our findings indicate that partial rejuvenation of bone marrow from aged rats with cells from young animals enhances the response to ischemic injury, potentially at the level of endothelial/vascular activation, providing insight into a novel approach ameliorate chronic vascular diseases.

Original languageEnglish
Pages (from-to)855-867
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume31
Issue number3
DOIs
Publication statusPublished - 01-03-2011

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Rejuvenation
Bone Marrow Cells
Brain Injuries
Bone Marrow
Microvessels
Microtubule-Associated Proteins
Nitric Oxide Synthase Type III
Cerebral Infarction
Inbred SHR Rats
Vascular Diseases
Protein Kinases
Nervous System
Blood Vessels
Blood Cells
Homeostasis
Chronic Disease
Cardiovascular Diseases
Transplantation
Stroke
Radiation

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Taguchi, Akihiko ; Zhu, Pengxiang ; Cao, Fang ; Kikuchi-Taura, Akie ; Kasahara, Yukiko ; Stern, David M. ; Soma, Toshihiro ; Matsuyama, Tomohiro ; Hata, Ryuji. / Reduced ischemic brain injury by partial rejuvenation of bone marrow cells in aged rats. In: Journal of Cerebral Blood Flow and Metabolism. 2011 ; Vol. 31, No. 3. pp. 855-867.
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Taguchi, A, Zhu, P, Cao, F, Kikuchi-Taura, A, Kasahara, Y, Stern, DM, Soma, T, Matsuyama, T & Hata, R 2011, 'Reduced ischemic brain injury by partial rejuvenation of bone marrow cells in aged rats', Journal of Cerebral Blood Flow and Metabolism, vol. 31, no. 3, pp. 855-867. https://doi.org/10.1038/jcbfm.2010.165

Reduced ischemic brain injury by partial rejuvenation of bone marrow cells in aged rats. / Taguchi, Akihiko; Zhu, Pengxiang; Cao, Fang; Kikuchi-Taura, Akie; Kasahara, Yukiko; Stern, David M.; Soma, Toshihiro; Matsuyama, Tomohiro; Hata, Ryuji.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 31, No. 3, 01.03.2011, p. 855-867.

Research output: Contribution to journalArticle

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AU - Hata, Ryuji

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