Reduced tumorigenicity and cell motility of a gastric carcinoma cell line by introduction of wild-type p53 gene

E. Kyo, H. Yokozaki, K. Yanagihara, W. Yasui, M. Ito, T. Sano, P. S. Lee, H. Saya, E. Tahara

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Wild-type or mutant human p53 gene was transfected into a human gastric carcinoma cell line MKN-1 which shares a mutant p53 allele. Transfected wild- type p53 reduced the colony forming efficiency and tumorigenicity of MKN-1 cells. However, no difference in expression of cell adhesion molecule, oncogenes and growth factors was observed among parent, wild-type p53 and mutant p53 transfectants. In motility assay, the wild-type p53 transfectants relative to the parental or mutant p53 transfectants exhibited a decreased motility, and HGF had a greater effect on the motility of the mutant p53 transfectants, but very little effect on the motility of either the parental or wild-type transfectants. In invasion assay, mutant p53 transfectants revealed the increased invasion ability into collagen gel. These results suggest that allele loss and point mutation of p53 gene may play a critical role not only in growth but also in invasion of gastric carcinoma cells.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalInternational journal of oncology
Volume3
Issue number2
DOIs
Publication statusPublished - 1993

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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