Reduction in the number of NADPH-diaphorase-positive cells in the cerebral cortex and striatum in aged rats

Kiyofumi Yamada, Yukihiro Noda, Yumiko Komori, Hisayoshi Sugihara, Takaaki Hasegawa, Toshitaka Nabeshima

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Abstract

Nitric oxide (NO) plays an important role as a diffusible messenger in learning and memory. To determine whether changes in NO production in the brain may be involved in aging-associated brain dysfunction, we measured the performance of aged rats in a radial arm maze task, and carried out histochemical examination of the changes in NADPH diaphorase (NADPH-d)-containing neurons in the brains of aged rats. The performance of aged rats (30 months old) in a radial arm maze task was significantly impaired compared to the performance of young rats (3 months old). The number of neurons containing NADPH-d reactivity in the cerebral cortex and striatum of aged rats was significantly reduced, by approximately 50 and 30%, respectively, compared to that in young rats. NO synthase activity in discrete brain regions of aged rats, i.e., in the cerebral cortex, striatum and hippocampus was not different from that in young rats, although the activity in the cerebellum of aged rats was significantly lower than that in young rats. These results suggest that the reduction in the number of NADPH-d-positive cells in the brains of aged rats may be involved in aging-associated learning impairment in rats.

Original languageEnglish
Pages (from-to)393-402
Number of pages10
JournalNeuroscience Research
Volume24
Issue number4
DOIs
Publication statusPublished - 01-01-1996

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NADPH Dehydrogenase
Cerebral Cortex
Brain
Nitric Oxide
Learning
Neurons
Nitric Oxide Synthase
Cerebellum
Hippocampus

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Yamada, Kiyofumi ; Noda, Yukihiro ; Komori, Yumiko ; Sugihara, Hisayoshi ; Hasegawa, Takaaki ; Nabeshima, Toshitaka. / Reduction in the number of NADPH-diaphorase-positive cells in the cerebral cortex and striatum in aged rats. In: Neuroscience Research. 1996 ; Vol. 24, No. 4. pp. 393-402.
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Reduction in the number of NADPH-diaphorase-positive cells in the cerebral cortex and striatum in aged rats. / Yamada, Kiyofumi; Noda, Yukihiro; Komori, Yumiko; Sugihara, Hisayoshi; Hasegawa, Takaaki; Nabeshima, Toshitaka.

In: Neuroscience Research, Vol. 24, No. 4, 01.01.1996, p. 393-402.

Research output: Contribution to journalArticle

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AU - Sugihara, Hisayoshi

AU - Hasegawa, Takaaki

AU - Nabeshima, Toshitaka

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