Regions of genomic instability on 22q11 and 11q23 as the etiology for the recurrent constitutional t(11;22)

Hiroki Kurahashi, Tamim H. Shaikh, Ping Hu, Bruce A. Roe, Beverly S. Emanuel, Marcia L. Budarf

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

The constitutional t(11;22)(q23;q11) is the only known recurrent, non-Robertsonian translocation. To analyze the genomic structure of the breakpoint, we have cloned the junction fragments from the der(11)and der(22) of a t(11;22) balanced carrier. On chromosome 11 the translocation occurs within a short, palindromic AT-rich region (ATRR). Likewise, the breakpoint on chromosome 22 has been localized within an ATRR that is part of a larger palindrome. Interestingly, the 22q11 breakpoint falls within one of the 'unclonable' gaps in the genomic sequence. Further, a sequenced chromosome 11 BAC clone, spanning the t(11;22) breakpoint in 11q23, is deleted within the palindromic ATRR, suggesting instability of this region in bacterial clones. Several unrelated t(11;22) families demonstrate similar breakpoints on both chromosomes, indicating that their translocations are within the same palindrome. It is likely that the palindromic ATRRs produce unstable DNA structures in 22q11 and 11q23 that are responsible for the recurrent t(11;22) translocation.

Original languageEnglish
Pages (from-to)1665-1670
Number of pages6
JournalHuman molecular genetics
Volume9
Issue number11
Publication statusPublished - 01-07-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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    Kurahashi, H., Shaikh, T. H., Hu, P., Roe, B. A., Emanuel, B. S., & Budarf, M. L. (2000). Regions of genomic instability on 22q11 and 11q23 as the etiology for the recurrent constitutional t(11;22). Human molecular genetics, 9(11), 1665-1670.