Regressive changes of astroglia in white matter lesions in cerebrovascular disease and Alzheimer's disease patients

Hidekazu Tomimoto, Ichiro Akiguchi, Hideaki Wakita, Toshihiko Suenaga, Shinichi Nakamura, Jun Kimura

Research output: Contribution to journalArticle

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Abstract

The pathogenesis of white matter lesions, which are frequently found in ischemic cerebrovascular disease and Alzheimer's disease, remains unclear. Using light and electron microscopic immunohistochemistry for glial fibrillary acidic protein (GFAP) as a marker, the present study focused on the role of astroglia which show characteristic morphological alterations. Of 29 brains of patients with cerebrovascular disease and Alzheimer's disease, 4 brains showed extensive swelling and vacuolation of white matter astroglia with their processes disintegrated and beaded (termed clasmatodendrosis). No such cells were observed in 6 control patients. Clasmatodendritic astroglia were not intensely eosinophilic using hematoxylin and eosin staining and included large lipophilic granules in their perikarya. These astroglia were immunoreactive for serum proteins such as immunoglobulins, fibrinogen and complement C3, Clq and C3d, as well as for proteins which are known to increase in reactive astroglia, such as vimentin, α-B crystallin, apolipoprotein-E and laminin. Double labeling for GFAP and microglial cell markers indicated that these cells were of astroglial lineage. Immunoelectron microscopy for GFAP revealed that clasmatodendritic astroglia had condensed chromatin, lysosomes and large membrane-bound osmiophilic cytoplasmic inclusions, which corresponded to the lipophilic granules observed with light microscopy. These cytochemical features collectively suggest that clasmatodendritic astroglia incorporate edema fluid and phagocytose cellular debris, and eventually degenerate as a result of cerebral edema.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalActa Neuropathologica
Volume94
Issue number2
DOIs
Publication statusPublished - 01-08-1997

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Cerebrovascular Disorders
Astrocytes
Alzheimer Disease
Glial Fibrillary Acidic Protein
Complement C3d
Light
Crystallins
Complement C3
Immunoelectron Microscopy
Inclusion Bodies
Brain Edema
Brain
Apolipoproteins E
Laminin
Vimentin
Hematoxylin
Eosine Yellowish-(YS)
Lysosomes
White Matter
Phagocytosis

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Tomimoto, Hidekazu ; Akiguchi, Ichiro ; Wakita, Hideaki ; Suenaga, Toshihiko ; Nakamura, Shinichi ; Kimura, Jun. / Regressive changes of astroglia in white matter lesions in cerebrovascular disease and Alzheimer's disease patients. In: Acta Neuropathologica. 1997 ; Vol. 94, No. 2. pp. 146-152.
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Regressive changes of astroglia in white matter lesions in cerebrovascular disease and Alzheimer's disease patients. / Tomimoto, Hidekazu; Akiguchi, Ichiro; Wakita, Hideaki; Suenaga, Toshihiko; Nakamura, Shinichi; Kimura, Jun.

In: Acta Neuropathologica, Vol. 94, No. 2, 01.08.1997, p. 146-152.

Research output: Contribution to journalArticle

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