Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors

Akemi Hanamura, Javier F. Cáceres, Akila Mayeda, B. Robert Franza, Adrian R. Krainer

Research output: Contribution to journalArticle

289 Citations (Scopus)

Abstract

The SR proteins are essential metazoan pre-mRNA splicing factors that can also influence the selection of alternative 5' splice sites in a concentration-dependent manner. Their activity in alternative splicing in vitro is antagonized by members of the hnRNP A/B family of proteins. The opposite effects of members of these two families of antagonistic splicing factors in vitro and upon overexpression in vivo suggest that changes in their relative levels may be a natural mechanism for the regulation of alternative splicing in vivo. One prediction of this model is that the ratios of these antagonists should vary in different cell types and in other situations in which cellular or viral transcripts are differentially spliced. We raised monoclonal antibodies specific for SF2/ASF and used them to measure the abundance of SF2/ASF protein and its isoforms, its phosphorylation state in vivo and during splicing in vitro, and its association with the spliceosome. SF2/ASF exists predominantly or exclusively in a highly phosphorylated state in vivo in all cell types examined, and unphosphorylated protein was not detectable. Unphosphorylated recombinant SF2/ASF becomes rapidly phosphorylated under splicing conditions in HeLa cell extracts and associates stably with one or more exons of β-globin pre-mRNA. This interaction appears to persist through the splicing reaction and SF2/ASF remains bound to spliced mRNA. We compared the distribution of SF2/ASF to that of its antagonist, hnRNP A1, in different rat tissues and in immortal and transformed cell lines. We found that the protein levels of these antagonistic splicing factors vary naturally over a very wide range, supporting the notion that changes in the ratio of these proteins can affect alternative splicing of a variety of pre-mRNAs in vivo.

Original languageEnglish
Pages (from-to)430-444
Number of pages15
JournalRNA
Volume4
Issue number4
Publication statusPublished - 01-04-1998
Externally publishedYes

Fingerprint

RNA Precursors
Alternative Splicing
RNA Splice Sites
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Proteins
Spliceosomes
Transformed Cell Line
Globins
Cell Extracts
HeLa Cells
Exons
Protein Isoforms
Monoclonal Antibodies
Phosphorylation
Messenger RNA
RNA Splicing Factors
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology

Cite this

Hanamura, A., Cáceres, J. F., Mayeda, A., Franza, B. R., & Krainer, A. R. (1998). Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. RNA, 4(4), 430-444.
Hanamura, Akemi ; Cáceres, Javier F. ; Mayeda, Akila ; Franza, B. Robert ; Krainer, Adrian R. / Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. In: RNA. 1998 ; Vol. 4, No. 4. pp. 430-444.
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Hanamura, A, Cáceres, JF, Mayeda, A, Franza, BR & Krainer, AR 1998, 'Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors', RNA, vol. 4, no. 4, pp. 430-444.

Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. / Hanamura, Akemi; Cáceres, Javier F.; Mayeda, Akila; Franza, B. Robert; Krainer, Adrian R.

In: RNA, Vol. 4, No. 4, 01.04.1998, p. 430-444.

Research output: Contribution to journalArticle

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Hanamura A, Cáceres JF, Mayeda A, Franza BR, Krainer AR. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. RNA. 1998 Apr 1;4(4):430-444.