Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus

Teiji Kuzuya, Yoshiaki Katano, Isao Nakano, Yoshiki Hirooka, Akihiro Itoh, Masatoshi Ishigami, Kazuhiko Hayashi, Takashi Honda, Hidemi Goto, Yuko Fujita, Rie Shikano, Yuji Muramatsu, Gustavo Bajotto, Tomohiro Tamura, Noriko Tamura, Yoshiharu Shimomura

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

The branched-chain α-keto acid dehydrogenase (BCKDH) complex is the most important regulatory enzyme in branched-chain amino acid (BCAA) catabolism. We examined the regulation of hepatic BCKDH complex activity in spontaneous type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats and Zucker diabetic fatty rats. Hepatic BCKDH complex activity in these rats was significantly lower than in corresponding control rats. The amount of BCKDH complex in OLETF rats corresponded to the total activity of the complex. Activity and abundance of the bound form of BCKDH kinase, which is responsible for inactivation of the complex, showed an inverse correlation to BCKDH complex activity in OLETF rats. Dietary supplementation of 5% BCAAs for 10 weeks markedly increased BCKDH complex activity, and decreased the activity and bound form of BCKDH kinase in the rats. These results suggest that BCAA catabolism in type 2 diabetes is downregulated and enhanced by BCAA supplementation.

Original languageEnglish
Pages (from-to)94-98
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume373
Issue number1
DOIs
Publication statusPublished - 15-08-2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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