Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus

Teiji Kuzuya; Yoshiaki Katano; Isao Nakano; Yoshiki Hirooka; Akihiro Itoh; Masatoshi Ishigami; Kazuhiko Hayashi; Takashi Honda; Hidemi Goto; Yuko Fujita; Rie Shikano; Yuji Muramatsu; Gustavo Bajotto; Tomohiro Tamura; Noriko Tamura; Yoshiharu Shimomura

doi:10.1016/j.bbrc.2008.05.167

Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus

Teiji Kuzuya
, Yoshiaki Katano
, Isao Nakano
, Yoshiki Hirooka
, Akihiro Itoh
, Masatoshi Ishigami
, Kazuhiko Hayashi
, Takashi Honda
, Hidemi Goto
, Yuko Fujita
, Rie Shikano
, Yuji Muramatsu
, Gustavo Bajotto
, Tomohiro Tamura
, Noriko Tamura
, Yoshiharu Shimomura

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

The branched-chain α-keto acid dehydrogenase (BCKDH) complex is the most important regulatory enzyme in branched-chain amino acid (BCAA) catabolism. We examined the regulation of hepatic BCKDH complex activity in spontaneous type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats and Zucker diabetic fatty rats. Hepatic BCKDH complex activity in these rats was significantly lower than in corresponding control rats. The amount of BCKDH complex in OLETF rats corresponded to the total activity of the complex. Activity and abundance of the bound form of BCKDH kinase, which is responsible for inactivation of the complex, showed an inverse correlation to BCKDH complex activity in OLETF rats. Dietary supplementation of 5% BCAAs for 10 weeks markedly increased BCKDH complex activity, and decreased the activity and bound form of BCKDH kinase in the rats. These results suggest that BCAA catabolism in type 2 diabetes is downregulated and enhanced by BCAA supplementation.

Original language	English
Pages (from-to)	94-98
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	373
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.05.167
Publication status	Published - 15-08-2008
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.05.167

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Kuzuya, T., Katano, Y., Nakano, I., Hirooka, Y., Itoh, A., Ishigami, M., Hayashi, K., Honda, T., Goto, H., Fujita, Y., Shikano, R., Muramatsu, Y., Bajotto, G., Tamura, T., Tamura, N., & Shimomura, Y. (2008). Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus. Biochemical and Biophysical Research Communications, 373(1), 94-98. https://doi.org/10.1016/j.bbrc.2008.05.167

@article{d6f12053c05e425fbb22e10439598f28,

title = "Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus",

abstract = "The branched-chain α-keto acid dehydrogenase (BCKDH) complex is the most important regulatory enzyme in branched-chain amino acid (BCAA) catabolism. We examined the regulation of hepatic BCKDH complex activity in spontaneous type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats and Zucker diabetic fatty rats. Hepatic BCKDH complex activity in these rats was significantly lower than in corresponding control rats. The amount of BCKDH complex in OLETF rats corresponded to the total activity of the complex. Activity and abundance of the bound form of BCKDH kinase, which is responsible for inactivation of the complex, showed an inverse correlation to BCKDH complex activity in OLETF rats. Dietary supplementation of 5\% BCAAs for 10 weeks markedly increased BCKDH complex activity, and decreased the activity and bound form of BCKDH kinase in the rats. These results suggest that BCAA catabolism in type 2 diabetes is downregulated and enhanced by BCAA supplementation.",

author = "Teiji Kuzuya and Yoshiaki Katano and Isao Nakano and Yoshiki Hirooka and Akihiro Itoh and Masatoshi Ishigami and Kazuhiko Hayashi and Takashi Honda and Hidemi Goto and Yuko Fujita and Rie Shikano and Yuji Muramatsu and Gustavo Bajotto and Tomohiro Tamura and Noriko Tamura and Yoshiharu Shimomura",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (17300208 and 20300216 to Y.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and a grant from the Uehara Memorial Foundation (to Y.S.).",

year = "2008",

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doi = "10.1016/j.bbrc.2008.05.167",

language = "English",

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Kuzuya, T , Katano, Y, Nakano, I, Hirooka, Y, Itoh, A, Ishigami, M, Hayashi, K, Honda, T, Goto, H, Fujita, Y, Shikano, R, Muramatsu, Y, Bajotto, G, Tamura, T, Tamura, N & Shimomura, Y 2008, 'Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus', Biochemical and Biophysical Research Communications, vol. 373, no. 1, pp. 94-98. https://doi.org/10.1016/j.bbrc.2008.05.167

TY - JOUR

T1 - Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus

AU - Kuzuya, Teiji

AU - Katano, Yoshiaki

AU - Nakano, Isao

AU - Hirooka, Yoshiki

AU - Itoh, Akihiro

AU - Ishigami, Masatoshi

AU - Hayashi, Kazuhiko

AU - Honda, Takashi

AU - Goto, Hidemi

AU - Fujita, Yuko

AU - Shikano, Rie

AU - Muramatsu, Yuji

AU - Bajotto, Gustavo

AU - Tamura, Tomohiro

AU - Tamura, Noriko

AU - Shimomura, Yoshiharu

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (17300208 and 20300216 to Y.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and a grant from the Uehara Memorial Foundation (to Y.S.).

PY - 2008/8/15

Y1 - 2008/8/15

N2 - The branched-chain α-keto acid dehydrogenase (BCKDH) complex is the most important regulatory enzyme in branched-chain amino acid (BCAA) catabolism. We examined the regulation of hepatic BCKDH complex activity in spontaneous type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats and Zucker diabetic fatty rats. Hepatic BCKDH complex activity in these rats was significantly lower than in corresponding control rats. The amount of BCKDH complex in OLETF rats corresponded to the total activity of the complex. Activity and abundance of the bound form of BCKDH kinase, which is responsible for inactivation of the complex, showed an inverse correlation to BCKDH complex activity in OLETF rats. Dietary supplementation of 5% BCAAs for 10 weeks markedly increased BCKDH complex activity, and decreased the activity and bound form of BCKDH kinase in the rats. These results suggest that BCAA catabolism in type 2 diabetes is downregulated and enhanced by BCAA supplementation.

AB - The branched-chain α-keto acid dehydrogenase (BCKDH) complex is the most important regulatory enzyme in branched-chain amino acid (BCAA) catabolism. We examined the regulation of hepatic BCKDH complex activity in spontaneous type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats and Zucker diabetic fatty rats. Hepatic BCKDH complex activity in these rats was significantly lower than in corresponding control rats. The amount of BCKDH complex in OLETF rats corresponded to the total activity of the complex. Activity and abundance of the bound form of BCKDH kinase, which is responsible for inactivation of the complex, showed an inverse correlation to BCKDH complex activity in OLETF rats. Dietary supplementation of 5% BCAAs for 10 weeks markedly increased BCKDH complex activity, and decreased the activity and bound form of BCKDH kinase in the rats. These results suggest that BCAA catabolism in type 2 diabetes is downregulated and enhanced by BCAA supplementation.

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SN - 0006-291X

VL - 373

SP - 94

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus

Abstract

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