Regulation of endothelin-1-induced interleukin-6 production by Ca2+ influx in human airway smooth muscle cells

Susumu Iwata, Satoru Ito, Mai Iwaki, Masashi Kondo, Toyokazu Sashio, Naoya Takeda, Masahiro Sokabe, Yoshinori Hasegawa, Hiroaki Kume

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Endothelin-1 is considered to be an important mediator in the pathophysiology of asthma because it induces contraction, hypertrophy, and proliferation in airway smooth muscle cells as well as inflammatory responses in the airway. Airway smooth muscle cells have been suggested to contribute to airway inflammation in asthma by producing cytokines. Nevertheless, the role of intracellular Ca2+ signal in cytokine production in human airway smooth muscle cells is still unclear. We investigated the mechanisms by which endothelin-1 induces production of interleukin (IL)-6, a pleiotropic cytokine, in primary cultured human airway smooth muscle cells. Levels of IL-6 protein and mRNA were significantly increased by endothelin-1 in dose- and time-dependent manners. Endothelin-1-induced IL-6 production was markedly attenuated by EGTA and various Ca2+ channel inhibitors such as 3,5-bis(trifluoromethyl)-1H-pyrazole derivative (BTP-2), 1-[β-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride (SKF96365), and nifedipine. Endothelin-1-induced increases in intracellular Ca2+ concentrations were significantly inhibited in Ca2+-free solution and by BTP-2, SKF96365, and nifedipine. The IL-6 synthesis was also inhibited by the extracellular signal-regulated kinase (ERK)1/2 inhibitor 1,4-diamino-2,3-dicyano-1,4-bis(o-aminophenylmercapto)-butadiene ethanolate (U0126) and the p38 inhibitor 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole (SB203580), but not by the c-Jun NH2-terminal kinase inhibitor anthra[1,9-cd]-pyrazol-6-(2H)-one (SP600125). Endothelin-1 significantly upregulated phosphorylation of ERK1/2 and p38 but blocking Ca2+ influx pathways did not inhibit either upregulation. These findings demonstrate that endothelin-1-induced IL-6 synthesis in airway smooth muscle cells occurs via two parallel but independent events that include Ca2+ influx and activation of ERK1/2 and p38.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalEuropean Journal of Pharmacology
Volume605
Issue number1-3
DOIs
Publication statusPublished - 01-03-2009

Fingerprint

Endothelin-1
Smooth Muscle Myocytes
Interleukin-6
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Nifedipine
Cytokines
Asthma
Mitogen-Activated Protein Kinase 3
JNK Mitogen-Activated Protein Kinases
Egtazic Acid
Mitogen-Activated Protein Kinase 1
Hypertrophy
Up-Regulation
Phosphorylation
Inflammation
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Iwata, Susumu ; Ito, Satoru ; Iwaki, Mai ; Kondo, Masashi ; Sashio, Toyokazu ; Takeda, Naoya ; Sokabe, Masahiro ; Hasegawa, Yoshinori ; Kume, Hiroaki. / Regulation of endothelin-1-induced interleukin-6 production by Ca2+ influx in human airway smooth muscle cells. In: European Journal of Pharmacology. 2009 ; Vol. 605, No. 1-3. pp. 15-22.
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Regulation of endothelin-1-induced interleukin-6 production by Ca2+ influx in human airway smooth muscle cells. / Iwata, Susumu; Ito, Satoru; Iwaki, Mai; Kondo, Masashi; Sashio, Toyokazu; Takeda, Naoya; Sokabe, Masahiro; Hasegawa, Yoshinori; Kume, Hiroaki.

In: European Journal of Pharmacology, Vol. 605, No. 1-3, 01.03.2009, p. 15-22.

Research output: Contribution to journalArticle

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T1 - Regulation of endothelin-1-induced interleukin-6 production by Ca2+ influx in human airway smooth muscle cells

AU - Iwata, Susumu

AU - Ito, Satoru

AU - Iwaki, Mai

AU - Kondo, Masashi

AU - Sashio, Toyokazu

AU - Takeda, Naoya

AU - Sokabe, Masahiro

AU - Hasegawa, Yoshinori

AU - Kume, Hiroaki

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Y1 - 2009/3/1

N2 - Endothelin-1 is considered to be an important mediator in the pathophysiology of asthma because it induces contraction, hypertrophy, and proliferation in airway smooth muscle cells as well as inflammatory responses in the airway. Airway smooth muscle cells have been suggested to contribute to airway inflammation in asthma by producing cytokines. Nevertheless, the role of intracellular Ca2+ signal in cytokine production in human airway smooth muscle cells is still unclear. We investigated the mechanisms by which endothelin-1 induces production of interleukin (IL)-6, a pleiotropic cytokine, in primary cultured human airway smooth muscle cells. Levels of IL-6 protein and mRNA were significantly increased by endothelin-1 in dose- and time-dependent manners. Endothelin-1-induced IL-6 production was markedly attenuated by EGTA and various Ca2+ channel inhibitors such as 3,5-bis(trifluoromethyl)-1H-pyrazole derivative (BTP-2), 1-[β-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride (SKF96365), and nifedipine. Endothelin-1-induced increases in intracellular Ca2+ concentrations were significantly inhibited in Ca2+-free solution and by BTP-2, SKF96365, and nifedipine. The IL-6 synthesis was also inhibited by the extracellular signal-regulated kinase (ERK)1/2 inhibitor 1,4-diamino-2,3-dicyano-1,4-bis(o-aminophenylmercapto)-butadiene ethanolate (U0126) and the p38 inhibitor 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole (SB203580), but not by the c-Jun NH2-terminal kinase inhibitor anthra[1,9-cd]-pyrazol-6-(2H)-one (SP600125). Endothelin-1 significantly upregulated phosphorylation of ERK1/2 and p38 but blocking Ca2+ influx pathways did not inhibit either upregulation. These findings demonstrate that endothelin-1-induced IL-6 synthesis in airway smooth muscle cells occurs via two parallel but independent events that include Ca2+ influx and activation of ERK1/2 and p38.

AB - Endothelin-1 is considered to be an important mediator in the pathophysiology of asthma because it induces contraction, hypertrophy, and proliferation in airway smooth muscle cells as well as inflammatory responses in the airway. Airway smooth muscle cells have been suggested to contribute to airway inflammation in asthma by producing cytokines. Nevertheless, the role of intracellular Ca2+ signal in cytokine production in human airway smooth muscle cells is still unclear. We investigated the mechanisms by which endothelin-1 induces production of interleukin (IL)-6, a pleiotropic cytokine, in primary cultured human airway smooth muscle cells. Levels of IL-6 protein and mRNA were significantly increased by endothelin-1 in dose- and time-dependent manners. Endothelin-1-induced IL-6 production was markedly attenuated by EGTA and various Ca2+ channel inhibitors such as 3,5-bis(trifluoromethyl)-1H-pyrazole derivative (BTP-2), 1-[β-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride (SKF96365), and nifedipine. Endothelin-1-induced increases in intracellular Ca2+ concentrations were significantly inhibited in Ca2+-free solution and by BTP-2, SKF96365, and nifedipine. The IL-6 synthesis was also inhibited by the extracellular signal-regulated kinase (ERK)1/2 inhibitor 1,4-diamino-2,3-dicyano-1,4-bis(o-aminophenylmercapto)-butadiene ethanolate (U0126) and the p38 inhibitor 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole (SB203580), but not by the c-Jun NH2-terminal kinase inhibitor anthra[1,9-cd]-pyrazol-6-(2H)-one (SP600125). Endothelin-1 significantly upregulated phosphorylation of ERK1/2 and p38 but blocking Ca2+ influx pathways did not inhibit either upregulation. These findings demonstrate that endothelin-1-induced IL-6 synthesis in airway smooth muscle cells occurs via two parallel but independent events that include Ca2+ influx and activation of ERK1/2 and p38.

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