Regulation of the Level of Vesl-1S/Homer-1a Proteins by Ubiquitin-Proteasome Proteolytic Systems

Hiroshi Ageta, Akihiko Kato, Shigetsugu Hatakeyama, Kei Ichi Nakayama, Yasushi Isojima, Hiroyuki Sugiyama

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The vesl-1S/homer-1a gene is up-regulated during seizure and long term potentiation. Other members of the Vesl family, Vesl-1L, -2, and -3, are constitutively expressed in the brain. We examined the regulatory mechanisms governing the expression level of Vesl-1S protein, either an exogenously introduced one in COS7 or human embryonic kidney 293T cells or an endogenous one in rat brain neurons in cultures. In both cases, application of proteasome inhibitors increased the amount of Vesl-1S protein but not that of Vesl-1L, -2, or -3 protein. Deletion analyses revealed that the C-terminal 11-amino acid region was responsible for the proteolysis of Vesl-1S by proteasomes. Application of proteasome inhibitors promoted ubiquitination of Vesl-1S protein but not that of the Vesl-1S deletion mutant, which evaded proteasome-mediated degradation. These results indicate that ubiquitin-proteasome systems are involved in the regulation of the expression level of Vesl-1S protein.

Original languageEnglish
Pages (from-to)15893-15897
Number of pages5
JournalJournal of Biological Chemistry
Volume276
Issue number19
DOIs
Publication statusPublished - 11-05-2001

Fingerprint

Proteasome Endopeptidase Complex
Ubiquitin
Proteasome Inhibitors
Proteins
Brain
Proteolysis
Long-Term Potentiation
HEK293 Cells
Ubiquitination
Neurons
Rats
Seizures
Genes
Homer Scaffolding Proteins
Kidney
Amino Acids
Degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ageta, Hiroshi ; Kato, Akihiko ; Hatakeyama, Shigetsugu ; Nakayama, Kei Ichi ; Isojima, Yasushi ; Sugiyama, Hiroyuki. / Regulation of the Level of Vesl-1S/Homer-1a Proteins by Ubiquitin-Proteasome Proteolytic Systems. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 19. pp. 15893-15897.
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Regulation of the Level of Vesl-1S/Homer-1a Proteins by Ubiquitin-Proteasome Proteolytic Systems. / Ageta, Hiroshi; Kato, Akihiko; Hatakeyama, Shigetsugu; Nakayama, Kei Ichi; Isojima, Yasushi; Sugiyama, Hiroyuki.

In: Journal of Biological Chemistry, Vol. 276, No. 19, 11.05.2001, p. 15893-15897.

Research output: Contribution to journalArticle

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