Relation of functional and morphological changes in mitochondria to myocardial contractile and relaxation reserves in asymptomatic to mildly symptomatic patients with hypertrophic cardiomyopathy

Kazumasa Unno, Satoshi Isobe, Hideo Izawa, Xian Wu Cheng, Masakazu Kobayashi, Akihiro Hirashiki, Takashi Yamada, Ken Harada, Satoru Ohshima, Akiko Noda, Kohzo Nagata, Katsuhiko Kato, Mitsuhiro Yokota, Toyoaki Murohara

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

AimsTo examine the relation between mitochondrial dysfunction and myocardial contractile and relaxation reserves in hypertrophic cardiomyopathy (HCM).Methods and resultsThirty HCM patients (LVEF ≥60%) underwent biventricular cardiac catheterization analysis both at rest and during atrial pacing as well as myocardial 99mTc-sestamibi scintigraphy at rest to calculate washout rate. Endomyocardial biopsy specimens were obtained for quantitative mRNA analysis and electron microscopy. The HCM patients were divided into two groups - group A: normal force-frequency relation and a pressure half-time (T1/2) of <30 ms (n = 15); group B: abnormal force-frequency relation or T1/2 of ≥30 ms (n = 15). The 99mTc- sestamibi washout rate was significantly correlated with T1/2 for all patients (r = 0.74, P < 0.01) and was also significantly greater in group B (29.2 ± 6.3%) than in group A (19.3 ± 3.1%). The abundance of mRNAs for mitochondrial electron transport-related enzymes was significantly higher in group A than in group B. Mitochondria showed a greater variation in size and were more disorganized in group B than in group A.

Original languageEnglish
Pages (from-to)1853-1862
Number of pages10
JournalEuropean Heart Journal
Volume30
Issue number15
DOIs
Publication statusPublished - 01-08-2009

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Heart Mitochondria
Hypertrophic Cardiomyopathy
Technetium Tc 99m Sestamibi
Cardiac Catheterization
Electron Transport
Radionuclide Imaging
Electron Microscopy
Mitochondria
Biopsy
Pressure
Messenger RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Unno, Kazumasa ; Isobe, Satoshi ; Izawa, Hideo ; Cheng, Xian Wu ; Kobayashi, Masakazu ; Hirashiki, Akihiro ; Yamada, Takashi ; Harada, Ken ; Ohshima, Satoru ; Noda, Akiko ; Nagata, Kohzo ; Kato, Katsuhiko ; Yokota, Mitsuhiro ; Murohara, Toyoaki. / Relation of functional and morphological changes in mitochondria to myocardial contractile and relaxation reserves in asymptomatic to mildly symptomatic patients with hypertrophic cardiomyopathy. In: European Heart Journal. 2009 ; Vol. 30, No. 15. pp. 1853-1862.
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abstract = "AimsTo examine the relation between mitochondrial dysfunction and myocardial contractile and relaxation reserves in hypertrophic cardiomyopathy (HCM).Methods and resultsThirty HCM patients (LVEF ≥60{\%}) underwent biventricular cardiac catheterization analysis both at rest and during atrial pacing as well as myocardial 99mTc-sestamibi scintigraphy at rest to calculate washout rate. Endomyocardial biopsy specimens were obtained for quantitative mRNA analysis and electron microscopy. The HCM patients were divided into two groups - group A: normal force-frequency relation and a pressure half-time (T1/2) of <30 ms (n = 15); group B: abnormal force-frequency relation or T1/2 of ≥30 ms (n = 15). The 99mTc- sestamibi washout rate was significantly correlated with T1/2 for all patients (r = 0.74, P < 0.01) and was also significantly greater in group B (29.2 ± 6.3{\%}) than in group A (19.3 ± 3.1{\%}). The abundance of mRNAs for mitochondrial electron transport-related enzymes was significantly higher in group A than in group B. Mitochondria showed a greater variation in size and were more disorganized in group B than in group A.",
author = "Kazumasa Unno and Satoshi Isobe and Hideo Izawa and Cheng, {Xian Wu} and Masakazu Kobayashi and Akihiro Hirashiki and Takashi Yamada and Ken Harada and Satoru Ohshima and Akiko Noda and Kohzo Nagata and Katsuhiko Kato and Mitsuhiro Yokota and Toyoaki Murohara",
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Unno, K, Isobe, S, Izawa, H, Cheng, XW, Kobayashi, M, Hirashiki, A, Yamada, T, Harada, K, Ohshima, S, Noda, A, Nagata, K, Kato, K, Yokota, M & Murohara, T 2009, 'Relation of functional and morphological changes in mitochondria to myocardial contractile and relaxation reserves in asymptomatic to mildly symptomatic patients with hypertrophic cardiomyopathy', European Heart Journal, vol. 30, no. 15, pp. 1853-1862. https://doi.org/10.1093/eurheartj/ehp184

Relation of functional and morphological changes in mitochondria to myocardial contractile and relaxation reserves in asymptomatic to mildly symptomatic patients with hypertrophic cardiomyopathy. / Unno, Kazumasa; Isobe, Satoshi; Izawa, Hideo; Cheng, Xian Wu; Kobayashi, Masakazu; Hirashiki, Akihiro; Yamada, Takashi; Harada, Ken; Ohshima, Satoru; Noda, Akiko; Nagata, Kohzo; Kato, Katsuhiko; Yokota, Mitsuhiro; Murohara, Toyoaki.

In: European Heart Journal, Vol. 30, No. 15, 01.08.2009, p. 1853-1862.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Relation of functional and morphological changes in mitochondria to myocardial contractile and relaxation reserves in asymptomatic to mildly symptomatic patients with hypertrophic cardiomyopathy

AU - Unno, Kazumasa

AU - Isobe, Satoshi

AU - Izawa, Hideo

AU - Cheng, Xian Wu

AU - Kobayashi, Masakazu

AU - Hirashiki, Akihiro

AU - Yamada, Takashi

AU - Harada, Ken

AU - Ohshima, Satoru

AU - Noda, Akiko

AU - Nagata, Kohzo

AU - Kato, Katsuhiko

AU - Yokota, Mitsuhiro

AU - Murohara, Toyoaki

PY - 2009/8/1

Y1 - 2009/8/1

N2 - AimsTo examine the relation between mitochondrial dysfunction and myocardial contractile and relaxation reserves in hypertrophic cardiomyopathy (HCM).Methods and resultsThirty HCM patients (LVEF ≥60%) underwent biventricular cardiac catheterization analysis both at rest and during atrial pacing as well as myocardial 99mTc-sestamibi scintigraphy at rest to calculate washout rate. Endomyocardial biopsy specimens were obtained for quantitative mRNA analysis and electron microscopy. The HCM patients were divided into two groups - group A: normal force-frequency relation and a pressure half-time (T1/2) of <30 ms (n = 15); group B: abnormal force-frequency relation or T1/2 of ≥30 ms (n = 15). The 99mTc- sestamibi washout rate was significantly correlated with T1/2 for all patients (r = 0.74, P < 0.01) and was also significantly greater in group B (29.2 ± 6.3%) than in group A (19.3 ± 3.1%). The abundance of mRNAs for mitochondrial electron transport-related enzymes was significantly higher in group A than in group B. Mitochondria showed a greater variation in size and were more disorganized in group B than in group A.

AB - AimsTo examine the relation between mitochondrial dysfunction and myocardial contractile and relaxation reserves in hypertrophic cardiomyopathy (HCM).Methods and resultsThirty HCM patients (LVEF ≥60%) underwent biventricular cardiac catheterization analysis both at rest and during atrial pacing as well as myocardial 99mTc-sestamibi scintigraphy at rest to calculate washout rate. Endomyocardial biopsy specimens were obtained for quantitative mRNA analysis and electron microscopy. The HCM patients were divided into two groups - group A: normal force-frequency relation and a pressure half-time (T1/2) of <30 ms (n = 15); group B: abnormal force-frequency relation or T1/2 of ≥30 ms (n = 15). The 99mTc- sestamibi washout rate was significantly correlated with T1/2 for all patients (r = 0.74, P < 0.01) and was also significantly greater in group B (29.2 ± 6.3%) than in group A (19.3 ± 3.1%). The abundance of mRNAs for mitochondrial electron transport-related enzymes was significantly higher in group A than in group B. Mitochondria showed a greater variation in size and were more disorganized in group B than in group A.

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U2 - 10.1093/eurheartj/ehp184

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