Relationship between long interspersed nuclear element-1 DNA methylation in leukocytes and dyslipidemia in the Japanese general population

Yoshiki Tsuboi, Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Genki Mizuno, Yuri Murase, Koji Ohashi, Hiroaki Ishikawa, Mari Kondo, Takashi Inoue, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: Aberrant global DNA methylation is involved in the development of several diseases, including cardiovascular disease (CVD). We investigated whether the methylation of long interspersed nuclear element-1 (LINE-1) in leukocytes is associated with dyslipidemia, a major risk factor for CVD, in the Japanese general population.Methods: We conducted a cross-sectional study consisting of 420 Japanese subjects (187 men and 233 women) without a clinical history of cancer, stroke, or ischemic heart disease. LINE-1 DNA methylation levels in leukocytes were measured using a pyrosequencing method.Results: Significantly higher odds ratios (ORs) for hypermethylation were observed in the high LDL cholesterol and high LDL/HDL ratio groups than the corresponding normal group (high LDLC group: OR, 1.88; 95% confidence interval [CI], 1.20–2.96, high LDL/HDL ratio group: OR, 1.90; 95% CI, 1.20–3.01). Subjects with 2 or more lipid abnormalities had significantly higher ORs for hypermethylation than those with no lipid abnormality (OR, 2.31; 95% CI, 1.11–4.82).Conclusion: LINE-1 DNA hypermethylation in leukocytes was associated with CVD risk profiles: high LDLC, high LDL/HDL ratio, and the degree of abnormal lipid metabolism.

Original languageEnglish
Pages (from-to)1231-1239
Number of pages9
JournalJournal of atherosclerosis and thrombosis
Volume25
Issue number12
DOIs
Publication statusPublished - 01-01-2018

Fingerprint

DNA Methylation
Dyslipidemias
Leukocytes
Odds Ratio
Cardiovascular Diseases
Population
Confidence Intervals
Lipids
Methylation
Lipid Metabolism
LDL Cholesterol
Myocardial Ischemia
Cross-Sectional Studies
Stroke
DNA
oxidized low density lipoprotein
Neoplasms

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine
  • Biochemistry, medical

Cite this

@article{2d327ea4a763434e982f0864491207d5,
title = "Relationship between long interspersed nuclear element-1 DNA methylation in leukocytes and dyslipidemia in the Japanese general population",
abstract = "Aim: Aberrant global DNA methylation is involved in the development of several diseases, including cardiovascular disease (CVD). We investigated whether the methylation of long interspersed nuclear element-1 (LINE-1) in leukocytes is associated with dyslipidemia, a major risk factor for CVD, in the Japanese general population.Methods: We conducted a cross-sectional study consisting of 420 Japanese subjects (187 men and 233 women) without a clinical history of cancer, stroke, or ischemic heart disease. LINE-1 DNA methylation levels in leukocytes were measured using a pyrosequencing method.Results: Significantly higher odds ratios (ORs) for hypermethylation were observed in the high LDL cholesterol and high LDL/HDL ratio groups than the corresponding normal group (high LDLC group: OR, 1.88; 95{\%} confidence interval [CI], 1.20–2.96, high LDL/HDL ratio group: OR, 1.90; 95{\%} CI, 1.20–3.01). Subjects with 2 or more lipid abnormalities had significantly higher ORs for hypermethylation than those with no lipid abnormality (OR, 2.31; 95{\%} CI, 1.11–4.82).Conclusion: LINE-1 DNA hypermethylation in leukocytes was associated with CVD risk profiles: high LDLC, high LDL/HDL ratio, and the degree of abnormal lipid metabolism.",
author = "Yoshiki Tsuboi and Hiroya Yamada and Eiji Munetsuna and Mirai Yamazaki and Genki Mizuno and Yuri Murase and Koji Ohashi and Hiroaki Ishikawa and Mari Kondo and Takashi Inoue and Shuji Hashimoto and Nobuyuki Hamajima and Koji Suzuki",
year = "2018",
month = "1",
day = "1",
doi = "10.5551/jat.43570",
language = "English",
volume = "25",
pages = "1231--1239",
journal = "Journal of Atherosclerosis and Thrombosis",
issn = "1340-3478",
publisher = "Japan Atherosclerosis Society",
number = "12",

}

Relationship between long interspersed nuclear element-1 DNA methylation in leukocytes and dyslipidemia in the Japanese general population. / Tsuboi, Yoshiki; Yamada, Hiroya; Munetsuna, Eiji; Yamazaki, Mirai; Mizuno, Genki; Murase, Yuri; Ohashi, Koji; Ishikawa, Hiroaki; Kondo, Mari; Inoue, Takashi; Hashimoto, Shuji; Hamajima, Nobuyuki; Suzuki, Koji.

In: Journal of atherosclerosis and thrombosis, Vol. 25, No. 12, 01.01.2018, p. 1231-1239.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Relationship between long interspersed nuclear element-1 DNA methylation in leukocytes and dyslipidemia in the Japanese general population

AU - Tsuboi, Yoshiki

AU - Yamada, Hiroya

AU - Munetsuna, Eiji

AU - Yamazaki, Mirai

AU - Mizuno, Genki

AU - Murase, Yuri

AU - Ohashi, Koji

AU - Ishikawa, Hiroaki

AU - Kondo, Mari

AU - Inoue, Takashi

AU - Hashimoto, Shuji

AU - Hamajima, Nobuyuki

AU - Suzuki, Koji

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Aim: Aberrant global DNA methylation is involved in the development of several diseases, including cardiovascular disease (CVD). We investigated whether the methylation of long interspersed nuclear element-1 (LINE-1) in leukocytes is associated with dyslipidemia, a major risk factor for CVD, in the Japanese general population.Methods: We conducted a cross-sectional study consisting of 420 Japanese subjects (187 men and 233 women) without a clinical history of cancer, stroke, or ischemic heart disease. LINE-1 DNA methylation levels in leukocytes were measured using a pyrosequencing method.Results: Significantly higher odds ratios (ORs) for hypermethylation were observed in the high LDL cholesterol and high LDL/HDL ratio groups than the corresponding normal group (high LDLC group: OR, 1.88; 95% confidence interval [CI], 1.20–2.96, high LDL/HDL ratio group: OR, 1.90; 95% CI, 1.20–3.01). Subjects with 2 or more lipid abnormalities had significantly higher ORs for hypermethylation than those with no lipid abnormality (OR, 2.31; 95% CI, 1.11–4.82).Conclusion: LINE-1 DNA hypermethylation in leukocytes was associated with CVD risk profiles: high LDLC, high LDL/HDL ratio, and the degree of abnormal lipid metabolism.

AB - Aim: Aberrant global DNA methylation is involved in the development of several diseases, including cardiovascular disease (CVD). We investigated whether the methylation of long interspersed nuclear element-1 (LINE-1) in leukocytes is associated with dyslipidemia, a major risk factor for CVD, in the Japanese general population.Methods: We conducted a cross-sectional study consisting of 420 Japanese subjects (187 men and 233 women) without a clinical history of cancer, stroke, or ischemic heart disease. LINE-1 DNA methylation levels in leukocytes were measured using a pyrosequencing method.Results: Significantly higher odds ratios (ORs) for hypermethylation were observed in the high LDL cholesterol and high LDL/HDL ratio groups than the corresponding normal group (high LDLC group: OR, 1.88; 95% confidence interval [CI], 1.20–2.96, high LDL/HDL ratio group: OR, 1.90; 95% CI, 1.20–3.01). Subjects with 2 or more lipid abnormalities had significantly higher ORs for hypermethylation than those with no lipid abnormality (OR, 2.31; 95% CI, 1.11–4.82).Conclusion: LINE-1 DNA hypermethylation in leukocytes was associated with CVD risk profiles: high LDLC, high LDL/HDL ratio, and the degree of abnormal lipid metabolism.

UR - http://www.scopus.com/inward/record.url?scp=85058965059&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058965059&partnerID=8YFLogxK

U2 - 10.5551/jat.43570

DO - 10.5551/jat.43570

M3 - Article

AN - SCOPUS:85058965059

VL - 25

SP - 1231

EP - 1239

JO - Journal of Atherosclerosis and Thrombosis

JF - Journal of Atherosclerosis and Thrombosis

SN - 1340-3478

IS - 12

ER -