Relationship between Mg2+-Induced Hexagonal Assembly of R-Form Lipopolysaccharides and Chemical Structure of Their R-Cores

Nobuo Kato, Michio Ohta, Nobuo Kido, Hideo Ito, Setsuko Naito, Yoshichika Arakawa, Takayuki Komatsu

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The relationship between formation of the Mg2+-induced hexagonal lattice structure by R-form lipopolysaccharides (LPS) and chemical structure of their R-cores was investigated using different kinds of R-form LPS from a series of mutants of Salmonella minnesota or S. typhimurium. The optimal experimental condition for formation of the hexagonal lattice structure was to suspend LPS preparations, from which cationic material was removed by electrodialysis, in 50 mM tris (hydroxymethyl) aminomethane buffer at pH 8.5 containing 10 mM MgCl2. Under this experimental condition, Rb1 LPS formed the hexagonal lattice structure with the lattice constant of 14.0±0.2 nm. Ra LPS, which possesses the full length of R-core, also formed the hexagonal lattice structure but its lattice constant was larger (18.1 ± 0.2 nm) than that of Rbi LPS (the lattice structure by Ra LPS was looser than that by Rbi LPS). All the other R-form LPS preparations tested, RcP+, RcP, Rd1P, and Re LPS, whose R-cores are shorter than that of Rbi LPS, did not form the hexagonal lattice structure, but formed membranous structures showing various shapes which consisted of multiple bilayer structures. Failure to form the hexagonal lattice structure was the common feature of these kinds of R-form LPS irrespective of temperature at which the LPS suspensions in 10 mM MgCl2-50 mM Tris buffer were incubated. From the results of the present study it was concluded that capability of R-form LPS to form the hexagonal lattice structure has a close correlation with the chemical structure of their R-cores.

Original languageEnglish
Pages (from-to)245-257
Number of pages13
JournalMICROBIOLOGY and IMMUNOLOGY
Volume34
Issue number3
DOIs
Publication statusPublished - 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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