Relationship between U83 gene variation in human herpesvirus 6 and secretion of the U83 gene product

R. Sjahril, Yuji Isegawa, T. Tanaka, K. Nakano, Tetsushi Yoshikawa, Y. Asano, A. Ohshima, K. Yamanishi, N. Sugimoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variations accumulated in variant A and in reactivated variant B after transplantation. None of the variant-A viruses encoded the signal peptide found in the B variant. U83 gene sequencing suggested that the variant A and B groups were separate, and that the variant B viruses could be further divided into the HST-Z29 type and another type with a shorter signal peptide. In a eukaryotic expression system, the HST-Z29 type of U83 gene product was secreted into the medium, a frame-shifted HST-Z29 type was partially secreted, and the variant-A type and a first-methionine knockout of the HST-Z29 type were not secreted.

Original languageEnglish
Pages (from-to)273-283
Number of pages11
JournalArchives of Virology
Volume154
Issue number2
DOIs
Publication statusPublished - 01-02-2009

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Human Herpesvirus 6
Genes
Protein Sorting Signals
Cercopithecine Herpesvirus 1
Chemotactic Factors
Methionine
Monocytes
Transplantation
Viruses
DNA

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Sjahril, R. ; Isegawa, Yuji ; Tanaka, T. ; Nakano, K. ; Yoshikawa, Tetsushi ; Asano, Y. ; Ohshima, A. ; Yamanishi, K. ; Sugimoto, N. / Relationship between U83 gene variation in human herpesvirus 6 and secretion of the U83 gene product. In: Archives of Virology. 2009 ; Vol. 154, No. 2. pp. 273-283.
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Sjahril, R, Isegawa, Y, Tanaka, T, Nakano, K, Yoshikawa, T, Asano, Y, Ohshima, A, Yamanishi, K & Sugimoto, N 2009, 'Relationship between U83 gene variation in human herpesvirus 6 and secretion of the U83 gene product', Archives of Virology, vol. 154, no. 2, pp. 273-283. https://doi.org/10.1007/s00705-008-0307-3

Relationship between U83 gene variation in human herpesvirus 6 and secretion of the U83 gene product. / Sjahril, R.; Isegawa, Yuji; Tanaka, T.; Nakano, K.; Yoshikawa, Tetsushi; Asano, Y.; Ohshima, A.; Yamanishi, K.; Sugimoto, N.

In: Archives of Virology, Vol. 154, No. 2, 01.02.2009, p. 273-283.

Research output: Contribution to journalArticle

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