Relationships between Helicobacter pylori infection status, endoscopic, histopathological findings, and cytokine production in the duodenum of Crohn's disease patients

Takafumi Ando, Osamu Watanabe, Kazuhiro Ishiguro, Osamu Maeda, Daisuke Ishikawa, Masaaki Minami, Motofusa Hasegawa, Shinya Kondo, Yasuyuki Goto, Naoki Omiya, Yasumasa Niwa, Hidemi Goto

Research output: Contribution to journalArticle

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Abstract

Background: The chronic inflammatory process in patients with Crohn's disease (CD) may affect any part of the gastrointestinal (GI) tract. The pathogenesis of CD involves immunological abnormalities, including deficient or excessive expression of cytokines. We examined Helicobacter pylori infection status, endoscopic and histopathological findings, and cytokine production in the duodenum of CD patients in comparison with controls. Methods: Thirty-eight CD patients underwent diagnostic upper GI endoscopy. Twelve age- and sex-matched health checkup examinees were used as controls. H. pylori infection status was assessed by the 13C-urea breath test. At the time of endoscopy, two biopsy specimens each were obtained from the second portion of the duodenum, one for hematoxylin-eosin staining and immunohistochemical analysis with anti-CD68 antibody, and one for in vitro organ culture. Interleukin (IL)-6 and -8 levels were measured in organ culture supernatant by enzyme-linked immunosorbent assay. Results: H. pylori infection was significantly (P < 0.05) more frequent in controls (42%) than in CD patients (8%). In the duodenum, erosions or ulcers were more frequent in CD patients (53%) than in controls (8%). Mononuclear cell infiltration in the duodenum was more severe in CD patients than in controls and IL-6 production was higher, whereas IL-8 production showed no significant difference. CD68+ cells in the duodenum were more prominent in CD patients than in controls. Conclusions: H. pylori infection is unlikely in CD patients, but they show immunological abnormalities in the duodenum, possibly from innate immune responses.

Original languageEnglish
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume23
Issue numberSUPPL. 2
DOIs
Publication statusPublished - 01-01-2008
Externally publishedYes

Fingerprint

Helicobacter Infections
Duodenum
Helicobacter pylori
Crohn Disease
Cytokines
Organ Culture Techniques
Interleukin-8
Interleukin-6
Breath Tests
Gastrointestinal Endoscopy
Hematoxylin
Eosine Yellowish-(YS)
Innate Immunity
Endoscopy
Ulcer
Urea
Gastrointestinal Tract
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Ando, Takafumi ; Watanabe, Osamu ; Ishiguro, Kazuhiro ; Maeda, Osamu ; Ishikawa, Daisuke ; Minami, Masaaki ; Hasegawa, Motofusa ; Kondo, Shinya ; Goto, Yasuyuki ; Omiya, Naoki ; Niwa, Yasumasa ; Goto, Hidemi. / Relationships between Helicobacter pylori infection status, endoscopic, histopathological findings, and cytokine production in the duodenum of Crohn's disease patients. In: Journal of Gastroenterology and Hepatology (Australia). 2008 ; Vol. 23, No. SUPPL. 2.
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Relationships between Helicobacter pylori infection status, endoscopic, histopathological findings, and cytokine production in the duodenum of Crohn's disease patients. / Ando, Takafumi; Watanabe, Osamu; Ishiguro, Kazuhiro; Maeda, Osamu; Ishikawa, Daisuke; Minami, Masaaki; Hasegawa, Motofusa; Kondo, Shinya; Goto, Yasuyuki; Omiya, Naoki; Niwa, Yasumasa; Goto, Hidemi.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 23, No. SUPPL. 2, 01.01.2008.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Relationships between Helicobacter pylori infection status, endoscopic, histopathological findings, and cytokine production in the duodenum of Crohn's disease patients

AU - Ando, Takafumi

AU - Watanabe, Osamu

AU - Ishiguro, Kazuhiro

AU - Maeda, Osamu

AU - Ishikawa, Daisuke

AU - Minami, Masaaki

AU - Hasegawa, Motofusa

AU - Kondo, Shinya

AU - Goto, Yasuyuki

AU - Omiya, Naoki

AU - Niwa, Yasumasa

AU - Goto, Hidemi

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Background: The chronic inflammatory process in patients with Crohn's disease (CD) may affect any part of the gastrointestinal (GI) tract. The pathogenesis of CD involves immunological abnormalities, including deficient or excessive expression of cytokines. We examined Helicobacter pylori infection status, endoscopic and histopathological findings, and cytokine production in the duodenum of CD patients in comparison with controls. Methods: Thirty-eight CD patients underwent diagnostic upper GI endoscopy. Twelve age- and sex-matched health checkup examinees were used as controls. H. pylori infection status was assessed by the 13C-urea breath test. At the time of endoscopy, two biopsy specimens each were obtained from the second portion of the duodenum, one for hematoxylin-eosin staining and immunohistochemical analysis with anti-CD68 antibody, and one for in vitro organ culture. Interleukin (IL)-6 and -8 levels were measured in organ culture supernatant by enzyme-linked immunosorbent assay. Results: H. pylori infection was significantly (P < 0.05) more frequent in controls (42%) than in CD patients (8%). In the duodenum, erosions or ulcers were more frequent in CD patients (53%) than in controls (8%). Mononuclear cell infiltration in the duodenum was more severe in CD patients than in controls and IL-6 production was higher, whereas IL-8 production showed no significant difference. CD68+ cells in the duodenum were more prominent in CD patients than in controls. Conclusions: H. pylori infection is unlikely in CD patients, but they show immunological abnormalities in the duodenum, possibly from innate immune responses.

AB - Background: The chronic inflammatory process in patients with Crohn's disease (CD) may affect any part of the gastrointestinal (GI) tract. The pathogenesis of CD involves immunological abnormalities, including deficient or excessive expression of cytokines. We examined Helicobacter pylori infection status, endoscopic and histopathological findings, and cytokine production in the duodenum of CD patients in comparison with controls. Methods: Thirty-eight CD patients underwent diagnostic upper GI endoscopy. Twelve age- and sex-matched health checkup examinees were used as controls. H. pylori infection status was assessed by the 13C-urea breath test. At the time of endoscopy, two biopsy specimens each were obtained from the second portion of the duodenum, one for hematoxylin-eosin staining and immunohistochemical analysis with anti-CD68 antibody, and one for in vitro organ culture. Interleukin (IL)-6 and -8 levels were measured in organ culture supernatant by enzyme-linked immunosorbent assay. Results: H. pylori infection was significantly (P < 0.05) more frequent in controls (42%) than in CD patients (8%). In the duodenum, erosions or ulcers were more frequent in CD patients (53%) than in controls (8%). Mononuclear cell infiltration in the duodenum was more severe in CD patients than in controls and IL-6 production was higher, whereas IL-8 production showed no significant difference. CD68+ cells in the duodenum were more prominent in CD patients than in controls. Conclusions: H. pylori infection is unlikely in CD patients, but they show immunological abnormalities in the duodenum, possibly from innate immune responses.

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JO - Journal of Gastroenterology and Hepatology (Australia)

JF - Journal of Gastroenterology and Hepatology (Australia)

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