Reliability of direct varicella zoster virus loop-mediated isothermal amplification method for rapid diagnosis of breakthrough varicella

Nagoya VZV study group

Research output: Contribution to journalArticle

Abstract

Background: Since patients with breakthrough varicella (BV) have mild symptoms, clinical diagnosis is difficult. In high vaccine coverage area, as BV occurs sporadically, point of care test is required for controlling varicella outbreak. In this study, the reliability of varicella zoster virus (VZV)-loop mediated isothermal amplification (LAMP) was evaluated for the rapid diagnosis of BV. Study design: A total of 328 swab samples collected from patients with suspected varicella were analyzed. For the laboratory diagnosis of varicella, VZV real-time PCR was carried out using DNA extracted from swab samples. Swab samples without DNA extraction were used for VZV-LAMP(direct-LAMP). Results: VZV infection was diagnosed by real-time PCR in 285 cases, including 105 natural varicella cases and 180 BV cases. VZV DNA was detected in 250 (87.8%) of the 285 cases by direct-LAMP. The presence and duration of fever, number of skin eruptions, and VZV DNA load were significantly lower in BV than natural varicella. The sensitivity of direct-LAMP for the diagnosis of varicella and BV was 93.3% and 84.4%, respectively. Conclusions: Direct LAMP was considered to be useful for rapid diagnosis of BV as it has several advantages such as low cost, ease and rapidity, as compared to real time PCR.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalJournal of Clinical Virology
Volume119
DOIs
Publication statusPublished - 01-10-2019

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Human Herpesvirus 3
Chickenpox
Real-Time Polymerase Chain Reaction
DNA
Point-of-Care Systems
Clinical Laboratory Techniques
Virus Diseases
Disease Outbreaks
Fever
Vaccines

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

@article{42c1de3e5f464f4f82e88d2e9c5b3d5f,
title = "Reliability of direct varicella zoster virus loop-mediated isothermal amplification method for rapid diagnosis of breakthrough varicella",
abstract = "Background: Since patients with breakthrough varicella (BV) have mild symptoms, clinical diagnosis is difficult. In high vaccine coverage area, as BV occurs sporadically, point of care test is required for controlling varicella outbreak. In this study, the reliability of varicella zoster virus (VZV)-loop mediated isothermal amplification (LAMP) was evaluated for the rapid diagnosis of BV. Study design: A total of 328 swab samples collected from patients with suspected varicella were analyzed. For the laboratory diagnosis of varicella, VZV real-time PCR was carried out using DNA extracted from swab samples. Swab samples without DNA extraction were used for VZV-LAMP(direct-LAMP). Results: VZV infection was diagnosed by real-time PCR in 285 cases, including 105 natural varicella cases and 180 BV cases. VZV DNA was detected in 250 (87.8{\%}) of the 285 cases by direct-LAMP. The presence and duration of fever, number of skin eruptions, and VZV DNA load were significantly lower in BV than natural varicella. The sensitivity of direct-LAMP for the diagnosis of varicella and BV was 93.3{\%} and 84.4{\%}, respectively. Conclusions: Direct LAMP was considered to be useful for rapid diagnosis of BV as it has several advantages such as low cost, ease and rapidity, as compared to real time PCR.",
author = "{Nagoya VZV study group} and Yuki Higashimoto and Yoshiki Kawamura and Ayumi Kuboshiki and Fumihiko Hattori and Hiroki Miura and Naoko Nishimura and Takao Ozaki and Masaru Ihira and Tetsushi Yoshikawa",
year = "2019",
month = "10",
day = "1",
doi = "10.1016/j.jcv.2019.07.009",
language = "English",
volume = "119",
pages = "53--58",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",

}

Reliability of direct varicella zoster virus loop-mediated isothermal amplification method for rapid diagnosis of breakthrough varicella. / Nagoya VZV study group.

In: Journal of Clinical Virology, Vol. 119, 01.10.2019, p. 53-58.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reliability of direct varicella zoster virus loop-mediated isothermal amplification method for rapid diagnosis of breakthrough varicella

AU - Nagoya VZV study group

AU - Higashimoto, Yuki

AU - Kawamura, Yoshiki

AU - Kuboshiki, Ayumi

AU - Hattori, Fumihiko

AU - Miura, Hiroki

AU - Nishimura, Naoko

AU - Ozaki, Takao

AU - Ihira, Masaru

AU - Yoshikawa, Tetsushi

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Background: Since patients with breakthrough varicella (BV) have mild symptoms, clinical diagnosis is difficult. In high vaccine coverage area, as BV occurs sporadically, point of care test is required for controlling varicella outbreak. In this study, the reliability of varicella zoster virus (VZV)-loop mediated isothermal amplification (LAMP) was evaluated for the rapid diagnosis of BV. Study design: A total of 328 swab samples collected from patients with suspected varicella were analyzed. For the laboratory diagnosis of varicella, VZV real-time PCR was carried out using DNA extracted from swab samples. Swab samples without DNA extraction were used for VZV-LAMP(direct-LAMP). Results: VZV infection was diagnosed by real-time PCR in 285 cases, including 105 natural varicella cases and 180 BV cases. VZV DNA was detected in 250 (87.8%) of the 285 cases by direct-LAMP. The presence and duration of fever, number of skin eruptions, and VZV DNA load were significantly lower in BV than natural varicella. The sensitivity of direct-LAMP for the diagnosis of varicella and BV was 93.3% and 84.4%, respectively. Conclusions: Direct LAMP was considered to be useful for rapid diagnosis of BV as it has several advantages such as low cost, ease and rapidity, as compared to real time PCR.

AB - Background: Since patients with breakthrough varicella (BV) have mild symptoms, clinical diagnosis is difficult. In high vaccine coverage area, as BV occurs sporadically, point of care test is required for controlling varicella outbreak. In this study, the reliability of varicella zoster virus (VZV)-loop mediated isothermal amplification (LAMP) was evaluated for the rapid diagnosis of BV. Study design: A total of 328 swab samples collected from patients with suspected varicella were analyzed. For the laboratory diagnosis of varicella, VZV real-time PCR was carried out using DNA extracted from swab samples. Swab samples without DNA extraction were used for VZV-LAMP(direct-LAMP). Results: VZV infection was diagnosed by real-time PCR in 285 cases, including 105 natural varicella cases and 180 BV cases. VZV DNA was detected in 250 (87.8%) of the 285 cases by direct-LAMP. The presence and duration of fever, number of skin eruptions, and VZV DNA load were significantly lower in BV than natural varicella. The sensitivity of direct-LAMP for the diagnosis of varicella and BV was 93.3% and 84.4%, respectively. Conclusions: Direct LAMP was considered to be useful for rapid diagnosis of BV as it has several advantages such as low cost, ease and rapidity, as compared to real time PCR.

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DO - 10.1016/j.jcv.2019.07.009

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VL - 119

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JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

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