Remodeling of glycans using glycosyltransferase genes

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Remodeling of glycans on the cell surface is an essential technique to analyze cellular function of lectin-glycan ligand interaction. Here we describe the methods to identify the responsible enzyme (glycosyltransferase) regulating the expression of the glycan of interest and to modulate the glycan expression by overexpressing the glycosyltransferase gene. For the identification of the responsible enzyme, we introduce a new method, CIRES (correlation index-based responsible-enzyme gene screening), that consists of statistical comparison of glycan expression profile obtained by flow cytometry and gene expression profile obtained by DNA microarray.

Original languageEnglish
Title of host publicationLectins
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages379-387
Number of pages9
ISBN (Print)9781493912919
DOIs
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1200
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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