Remodeling of glycans using glycosyltransferase genes

Yuko Naito-Matsui; Hiromu Takematsu

doi:10.1007/978-1-4939-1292-6_33

Remodeling of glycans using glycosyltransferase genes

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Remodeling of glycans on the cell surface is an essential technique to analyze cellular function of lectin-glycan ligand interaction. Here we describe the methods to identify the responsible enzyme (glycosyltransferase) regulating the expression of the glycan of interest and to modulate the glycan expression by overexpressing the glycosyltransferase gene. For the identification of the responsible enzyme, we introduce a new method, CIRES (correlation index-based responsible-enzyme gene screening), that consists of statistical comparison of glycan expression profile obtained by flow cytometry and gene expression profile obtained by DNA microarray.

Original language	English
Title of host publication	Lectins
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	379-387
Number of pages	9
ISBN (Print)	9781493912919
DOIs	https://doi.org/10.1007/978-1-4939-1292-6_33
Publication status	Published - 2014
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1200
ISSN (Print)	1064-3745

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-1292-6_33

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