Renal ENaC subunit, Na-K-2Cl and Na-Cl cotransporter abundances in aged, water-restricted F344 x Brown Norway rats

Y. Tian, S. Riazi, O. Khan, J. D. Klein, Yoshihisa Sugimura, J. G. Verbalis, C. A. Ecelbarger

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Renal sodium reabsorption is a key determinant of final urine concentration. Our aim was to determine whether differences existed between aged and young rats in their response to water restriction with regard to the regulation of abundance of any of the major distal renal sodium transporter proteins. Male Fisher 344 x Brown Norway (F344 x BN) rats of 3-, 10-, 24-, or 31 months of age (3M, 10M, 24M, or 31M) were either water restricted (WR) for 5 days or control (ad libitum water). Major renal sodium transporters and channel subunits were evaluated by immunoblotting and immunohistochemistry. Age did not significantly affect plasma arginine vasopressin or aldosterone levels, but renin activity was only 8% in 31M-WR rats relative to 3M-WR (P<0.05). Extreme aging (31M) led to decreased outer medullary abundance of the bumetanide-sensitive Na-K-2Cl cotransporter and decreased cortical abundance of the β- and γ-subunits (70-kDa band) of the epithelial sodium channel (ENaC) (P<0.05). Water restriction significantly (P<0.05) increased the abundance of Na-K-2Cl cotransporter (NKCC2) and Na-Cl cotransporter (NCC) across ages. However, these increases were significantly blunted as rats aged. Mean band densities were increased in WR rats (relative to age controls) by 54 and 106% at 3M, but only 25 and 29% at 24M and 0 and 6% at 31M for NKCC2 and NCC, respectively. Aged F344 x BN rats have reduced basal distal tubular renal sodium transporter abundances and blunted upregulation during water restriction, which may contribute to decreased urinary concentrating capacity.

Original languageEnglish
Pages (from-to)304-312
Number of pages9
JournalKidney International
Volume69
Issue number2
DOIs
Publication statusPublished - 01-01-2006

Fingerprint

Sodium-Potassium-Chloride Symporters
Norway
Kidney
Water
Member 3 Solute Carrier Family 12
Sodium
Member 1 Solute Carrier Family 12
Epithelial Sodium Channels
Bumetanide
Arginine Vasopressin
Sodium Channels
Aldosterone
Immunoblotting
Renin
Up-Regulation
Immunohistochemistry
Urine

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Tian, Y. ; Riazi, S. ; Khan, O. ; Klein, J. D. ; Sugimura, Yoshihisa ; Verbalis, J. G. ; Ecelbarger, C. A. / Renal ENaC subunit, Na-K-2Cl and Na-Cl cotransporter abundances in aged, water-restricted F344 x Brown Norway rats. In: Kidney International. 2006 ; Vol. 69, No. 2. pp. 304-312.
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abstract = "Renal sodium reabsorption is a key determinant of final urine concentration. Our aim was to determine whether differences existed between aged and young rats in their response to water restriction with regard to the regulation of abundance of any of the major distal renal sodium transporter proteins. Male Fisher 344 x Brown Norway (F344 x BN) rats of 3-, 10-, 24-, or 31 months of age (3M, 10M, 24M, or 31M) were either water restricted (WR) for 5 days or control (ad libitum water). Major renal sodium transporters and channel subunits were evaluated by immunoblotting and immunohistochemistry. Age did not significantly affect plasma arginine vasopressin or aldosterone levels, but renin activity was only 8{\%} in 31M-WR rats relative to 3M-WR (P<0.05). Extreme aging (31M) led to decreased outer medullary abundance of the bumetanide-sensitive Na-K-2Cl cotransporter and decreased cortical abundance of the β- and γ-subunits (70-kDa band) of the epithelial sodium channel (ENaC) (P<0.05). Water restriction significantly (P<0.05) increased the abundance of Na-K-2Cl cotransporter (NKCC2) and Na-Cl cotransporter (NCC) across ages. However, these increases were significantly blunted as rats aged. Mean band densities were increased in WR rats (relative to age controls) by 54 and 106{\%} at 3M, but only 25 and 29{\%} at 24M and 0 and 6{\%} at 31M for NKCC2 and NCC, respectively. Aged F344 x BN rats have reduced basal distal tubular renal sodium transporter abundances and blunted upregulation during water restriction, which may contribute to decreased urinary concentrating capacity.",
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Renal ENaC subunit, Na-K-2Cl and Na-Cl cotransporter abundances in aged, water-restricted F344 x Brown Norway rats. / Tian, Y.; Riazi, S.; Khan, O.; Klein, J. D.; Sugimura, Yoshihisa; Verbalis, J. G.; Ecelbarger, C. A.

In: Kidney International, Vol. 69, No. 2, 01.01.2006, p. 304-312.

Research output: Contribution to journalArticle

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AU - Riazi, S.

AU - Khan, O.

AU - Klein, J. D.

AU - Sugimura, Yoshihisa

AU - Verbalis, J. G.

AU - Ecelbarger, C. A.

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